NCT07378787

Brief Summary

The goal of this randomised controlled trial is to investigate whether Time-Restricted Eating (TRE) combined with a Low Glycaemic Index Mediterranean Diet can improve cognitive function, metabolic health, and biological ageing in late perimenopausal and menopausal women aged 45-75 years with a BMI ≥23 kg/m². It will consist of a 12-week intervention followed by a 12 week follow-up period. Researchers will compare a group following the 16:8 time-restricted eating schedule and Low Glycaemic Index Mediterranean Diet to a control group (waiting list) receiving no dietary or meal-timing instructions to determine if these dietary strategies influence cognitive function, assessed via brain-derived neurotropic factor (BDNF) and cognitive function tests, metabolic outcomes, biological age, the gut microbiome, tender and swollen joint count and global health. Participants will: Follow a 16:8 time-restricted eating schedule (eating within an 8-hour window and fasting for 16 hours) if assigned to the intervention group. Adhere to a tailored Low Glycaemic Index Mediterranean Diet and track meals using the Nutrium app. Attend study visits at the University of Nicosia at Week 0, Week 12, and Week 24 for various assessments. Provide biological samples, including blood samples at five specific intervals and stool samples at Week 0 and Week 12. Complete multiple questionnaires regarding diet, lifestyle, sleep quality, physical activity, tender and swollen joints, and quality of life. Undergo physical and cognitive assessments, such as CNS Vital Signs tests, blood pressure measurements, and body composition analysis. The two groups will be compared to understand if the intervention improves outcomes and can thus be applied in this population group.

Trial Health

80
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
72

participants targeted

Target at P50-P75 for not_applicable

Timeline
33mo left

Started Jan 2026

Typical duration for not_applicable

Geographic Reach
2 countries

2 active sites

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress10%
Jan 2026Jan 2029

Study Start

First participant enrolled

January 17, 2026

Completed
6 days until next milestone

First Submitted

Initial submission to the registry

January 23, 2026

Completed
7 days until next milestone

First Posted

Study publicly available on registry

January 30, 2026

Completed
3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 20, 2029

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

January 20, 2029

Last Updated

January 30, 2026

Status Verified

January 1, 2026

Enrollment Period

3 years

First QC Date

January 23, 2026

Last Update Submit

January 23, 2026

Conditions

Keywords

Peri-menopausemenopauseintermittent fastinglow glycaemic-index dietwomentime-restricted eatingbiological ageingmetabolic markerscognitive functioncognitiontender jointsswollen jointsnutritionmicrobiomeMediterranean dietGlycanAgerandomized controlled trialBDNFinsulin resistancemiddle-agedglobal health

Outcome Measures

Primary Outcomes (1)

  • Brain-derived neurotrophic factor concentration

    Changes between the intervention and control group in the concentration of brain-derived neurotrophic factor assessed by Enzyme-linked Immunosorbent Assay

    Week 0 and Week 12 for the intervention period and Week 24 for the follow-up period

Secondary Outcomes (12)

  • Cognitive function

    Week 0 and Week 12 for the intervention period and Week 24 for the follow-up period

  • Insulin resistance

    Week 0 and Week 12 for the intervention period

  • Lipid profile

    Week 0 and Week 12 for the intervention period

  • C-reactive protein (CRP)

    Week 0 and Week 12 for the intervention period

  • IgG N-glycome composition analysis to assess GlycanAge

    The test will be conducted at Week 0, 6, 12, 18, and 24 to monitor progressive changes in biological ageing throughout the 6-month study period.

  • +7 more secondary outcomes

Other Outcomes (2)

  • Physical activity assessment

    Week 0 and Week 12 for the intervention period and Week 24 for the follow-up period

  • Nutritional Assessment and adherence to the dietary intervention

    Intervention group: weekly monitoring between Week 1 to Week 12. Control group: 3-day food diary at week 1, week 6 and Week 12. All participants: 3-day food diary at Week 18 and Week 24. Adherence to the intervention: week 1, 6, 12, 24

Study Arms (2)

Time-Restricted Low Glycaemic Index Diet

EXPERIMENTAL

Participants in the Time-Restricted-Low-Glycaemic Index group will follow a 16:8 intermittent fasting regimen, where all meals are consumed within an 8-hour eating window. Their diet will also consist of low-glycaemic index foods following the principles of the Mediterranean diet. They will provided with individualized dietary plans through the Nutrium App and followed up regularly through the app.

Other: Time-Restricted Low-Glycaemic Index Diet

Control group

NO INTERVENTION

The Control Group will receive no dietary intervention during the 6-month trial but will be placed on a waiting list for future guidance if requested. Their dietary intake will be recorded through the Nutrium App.

Interventions

The combination of a 16:8 Time-Restricted eating period with a low glycaemic index Mediterranean diet

Also known as: intermittent fasting, low glycaemic diet, Mediterranean diet, 16:8
Time-Restricted Low Glycaemic Index Diet

Eligibility Criteria

Age45 Years - 75 Years
Sexfemale(Gender-based eligibility)
Gender Eligibility DetailsFemale
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Women in late perimenopausal or menopausal status Note: Late perimenopause is defined as two or more skipped cycles and at least 60 days of amenorrhea. Recent blood tests showing elevated FSH levels support eligibility for late perimenopause. Menopause is defined as 12 consecutive months without a menstrual cycle.
  • BMI ≥23 kg/m2
  • Age 45-75 years old
  • If using any medication, the dose should be stable for the previous 12 months
  • Able to use technology and follow the dietary plan

You may not qualify if:

  • Diagnosis of mild cognitive impairment (MCI) or Alzheimer's disease (AD). Note: (Self-reported memory complaints are allowed).
  • On hormone replacement therapy (HRT)
  • Medical conditions that significantly affect cognitive or metabolic function including diagnosis of type 1 diabetes, severe metabolic disorders, cardiovascular disease, or neurodegenerative conditions, diagnosed eating disorders or severe mental health conditions.
  • Currently enrolled in another intervention.
  • Severe food allergies or dietary restrictions that would prevent adherence to the study diet.
  • History of substance abuse.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

Genos Ltd

Zagreb, 10000, Croatia

ACTIVE NOT RECRUITING

University of Nicosia

Nicosia, Engomi, 1700, Cyprus

RECRUITING

Related Publications (1)

  • Demetriou CA, Hileti D, Onisiphorou E, Kazafanioti C, Alogakos M, Vardakastani D, Christofidou E, Andreou EP, Giannaki CD, Stavrinou PS, Philippou P, Constantinidou F, Philippou E. Associations Between Chrono-Nutrition Behaviours and Cognitive Function in Middle-Aged Adults: The NUTRICO Cross-Sectional Cohort Study. Nutr Bull. 2025 Jun;50(2):262-277. doi: 10.1111/nbu.70000. Epub 2025 Feb 24.

    PMID: 39992029BACKGROUND

Related Links

MeSH Terms

Conditions

Intermittent FastingInsulin Resistance

Interventions

Diet, Mediterranean

Condition Hierarchy (Ancestors)

FastingFeeding BehaviorBehaviorHyperinsulinismGlucose Metabolism DisordersMetabolic DiseasesNutritional and Metabolic Diseases

Intervention Hierarchy (Ancestors)

Diet, Plant-BasedDiet TherapyNutrition TherapyTherapeuticsDietNutritional Physiological PhenomenaDiet, Food, and NutritionPhysiological Phenomena

Study Officials

  • Elena Philippou, PhD

    University of Nicosia

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Elena Philippou, PhD

CONTACT

Francesco Colelli, MSc

CONTACT

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
NONE
Purpose
PREVENTION
Intervention Model
PARALLEL
Model Details: Randomized controlled trial
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Associate Professor Nutrition-Dietetics

Study Record Dates

First Submitted

January 23, 2026

First Posted

January 30, 2026

Study Start

January 17, 2026

Primary Completion (Estimated)

January 20, 2029

Study Completion (Estimated)

January 20, 2029

Last Updated

January 30, 2026

Record last verified: 2026-01

Data Sharing

IPD Sharing
Will share

De-identified individual participant data underlying the results reported in the published article (including cognitive test scores, metabolic markers, and N-glycome markers) will be made available to qualified researchers. Data will be shared only after a formal request and upon approval of a methodologically sound research proposal. To protect participant privacy in accordance with GDPR and the Cyprus National Bioethics Committee, all shared data will be coded, and researchers must sign a Data Use Agreement (DUA) ensuring they will not attempt to re-identify any individual.

Shared Documents
STUDY PROTOCOL, SAP, ICF
Time Frame
Data will be available beginning 6 months after the publication of the primary manuscript and ending 10 years after the study start date.
Access Criteria
De-identified individual participant data (IPD) and supporting documents (Study Protocol and SAP) will be made available to qualified academic researchers upon request. To gain access, researchers must submit a detailed research proposal and a methodologically sound analysis plan for review by the NutriAge trial steering committee. Approval is contingent on the scientific merit of the proposal and the feasibility of the analysis. Before data transfer, the requesting institution must execute a Data Use Agreement (DUA) to ensure strict adherence to GDPR standards and to prevent any attempts at participant re-identification.

Locations