Evaluation of Bacterial Contamination and Antimicrobial Efficacy on Charcoal-Infused vs, Standard Nylon Toothbrush Bristles.

NCT07365748 | Completed

• 1 other identifier: SUEZ Med - IRB Approval No: 95
• Study Type: interventional
• Target: 36
• Locations: 1 country
• Sites: 1

Brief Summary

This study compares two types of toothbrushes to see which one stays cleaner after use. Researchers want to know if toothbrushes with charcoal-infused bristles have fewer bacteria than standard toothbrushes with nylon bristles. Participants in this study are adults and children who do not have gum disease or other major health issues. Everyone in the study will be assigned one of two types of toothbrushes:

• An eco-friendly toothbrush with charcoal bristles.
• A standard toothbrush with nylon bristles. Participants will use their assigned toothbrush at home for two specific time periods (one day and one week). They will follow simple instructions on how to brush and store the toothbrush. After each time period, they will return the toothbrush to the researchers. The research team will test the bristles in a lab to count the bacteria. They will also measure how well the charcoal bristles stop bacteria from growing compared to the nylon bristles. This will help determine if charcoal toothbrushes are a more sanitary and eco-friendly option for daily oral care.

Conditions

to Evaluate Antimicrobial Effect of Eco-friendly Toothbrush

Keywords

Eco-Friendly toothbrush , Charcoal toothbrush , Antimicrobial

Outcome Measures

Primary Outcomes (1)

• Colony-forming units (CFU)

  Bacterial load will be quantified as CFU per milliliter (CFU/mL) from toothbrush bristles after 1 day and 1 week of use. One-third of the bristles will be aseptically cut, placed in 5 mL sterile saline, mixed to elute bacteria, then 0.1 mL will be plated onto blood agar. Plates will be incubated under anaerobic conditions for 48 hours, then colonies will be counted and recorded as CFU/mL.

  10 days

Study Arms (4)

Antimicrobial effect of Eco-friendly tooth brush in children

EXPERIMENTAL

this arm will use the Eco-friendly toothbrush and will be evaluated for its antimicrobial effect

Device: Eco-friendly toothbrush for children

Antimicrobial effect of Eco-friendly tooth brush in adults

EXPERIMENTAL

this arm will use the eco-friendly toothbrush and tested for its antimicrobial effect in adults

Device: Eco-friendly toothbrush in adults

Nylon bristles toothbrush in children

ACTIVE COMPARATOR

this arm will use the nylon toothbrush bristles and be evaluated for its antibacterial effect

Device: nylon bristles toothbrush in children

nylon toothbrush bristles in adults

ACTIVE COMPARATOR

this arm will use the nylon bristles toothbrush and be evaluated for its antimicrobial effect

Device: Nylon bristles toothbrush in adults

Interventions

nylon bristles toothbrush in children DEVICE

a normal man made toothbrush used by children

Nylon bristles toothbrush in children

Nylon bristles toothbrush in adults DEVICE

a normal man made toothbrush used by adults

nylon toothbrush bristles in adults

Eco-friendly toothbrush in adults DEVICE

to see the antimicrobial effect of eco-friendly toothbrush in adults

Antimicrobial effect of Eco-friendly tooth brush in adults

Eco-friendly toothbrush for children DEVICE

it is an eco-friendly charcoal toothbrush assumed to have antibacterial property in children

Antimicrobial effect of Eco-friendly tooth brush in children

Eligibility Criteria

• Age: 8 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64)

You may qualify if:

• Participants free of any systemic diseases.
• Participants falling into one of two specific age groups:
• A) Children aged 8 to 11 years.
• B) Adults aged 18 to 35 years.
• Willingness to use the assigned toothbrush type (Eco-friendly charcoal or standard nylon).
• Ability to follow study instructions regarding brushing technique and storage.

You may not qualify if:

• Patients with open carious lesions.
• Participants with poor oral hygiene, defined as plaque index scores greater than 2.
• Participants with severe gingivitis, defined as gingival index score greater than 2.
• Current throat infections.
• Irregular brushing frequency (not brushing twice daily).
• Current use of mouthwash or antibacterial toothpastes.
• Current smokers.
• Medically compromised individuals.
• Unwillingness to use a charcoal Eco-friendly toothbrush.

Sponsors & Collaborators

• MTI University: lead

Study Sites (1)

Dental clinic

Cairo, 11585, Egypt

Location

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: INVESTIGATOR
• Purpose: PREVENTION
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Lecturer of Pediatric Dentistry & Dental Public Health at MTI University

Study Record Dates

• First Submitted: January 4, 2026
• First Posted: January 26, 2026
• Study Start: November 11, 2025
• Primary Completion: December 20, 2025
• Study Completion: January 1, 2026
• Last Updated: January 26, 2026

Record last verified: 2026-01

Data Sharing

• IPD Sharing: Will share
• Shared Documents: ICF, CSR
• Time Frame: october 2025 to october 2026

Locations

EG (1)