The Antiplaque and Antigingivitis Effect of Pure Green Tea Mouth Wash Compared to Placebo and Essential Oils Mouth Washes

NCT07364565 | Recruiting

• 2 other identifiers: 425/20252025/185-1
• Study Type: interventional
• Target: 44
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this clinical trial is to to evaluate the antiplaque and antigingivitis effect of pure green tea mouthwash compared to placebo and essential oils mouthwashes in healthy patients. The main questions it aims to answer are:

• Does pure green tea mouthwash effectively reduce dental plaque?
• Does pure green tea mouthwash reduce gingival inflammation? Researchers will compare pure green tea mouthwash to Listerine Total Care, Listerine Green Tea, and placebo to see if there is a difference in plaque accumulation and gum inflammation reduction. Participants will be prohibited from using any sort of oral hygiene parctice for the whole study period (3 weeks). Instead, they will rinse with 10 ml of their assigned mouthwash twice daily for 30 seconds. Plaque index, gingival index, and staining index will be examined at multiple intervals and participants will rate their sense of oral freshness and good breath.

Conditions

Dental Plaque; Patient Reported Outcome; Gingival Inflammation and Bleeding; Gingival Inflammation; Gingivitis; Gingival Bleeding

Keywords

Green tea; Gingival inflammation; Plaque index; Essential oils; Mouthwash; Bleeding on probing; Camellia sinensis; Herbal medicine; Periodontal health

Outcome Measures

Primary Outcomes (1)

• Turesky modification of the Quigley-Hein plaque index (TM-QHPI)

  will be recorded at six aspects on each tooth (mesialbuccal, midbuccal, distobuccal, mesiolingual, midlingual, and distolingual). 0: No plaque. 1. Separate flecks of plaque at the gumline (cervical margin). 2. A thin, continuous band (up to 1 mm) at the gumline. 3. A band wider than 1 mm but covering less than one-third of the tooth crown. 4. Plaque covering one-third to two-thirds of the crown. 5. Plaque covering two-thirds or more of the crown.

  On days 0, 7, 14, and 21

Secondary Outcomes (3)

• Gingival Index (GI)

  On days 0, 7, 14, and 21

• The Discoloration Index (DI)

  On days 0, 7, 14, and 21

• Patient-reported outcome

  On days 7, 14, and 21

Study Arms (4)

Pure Green Tea Mouthwash

EXPERIMENTAL

Participants in this group will use a mouthwash prepared from pure green tea extract. The solution will be brewed by infusing 1 g of green tea leaves in 100 ml of boiling deionized water for 20 minutes, cooled, filtered, and stored in brown glass bottles.

Drug: Pure Green Tea Mouthwash

Listerine Total Care Mouthwash

ACTIVE COMPARATOR

Participants in this group will use Listerine Total Care essential oils mouthwash, containing eucalyptol, menthol, methyl salicylate, and thymol.

Drug: Listerine Total Care Mouthwash

Listerine Green Tea Mouthwash

ACTIVE COMPARATOR

Participants will use Listerine Green Tea mouthwash, an essential oil-based product enhanced with green tea extract.

Drug: Listerine Green Tea Mouthwash

Placebo Mouthwash

PLACEBO COMPARATOR

The solution contains only water and additive flavor with no active anti-inflammatory ingredients.

Other: Placebo Mouthwash

Interventions

Placebo Mouthwash OTHER

Participants will rinse with 10 ml of a placebo solution consisting of pure water with a flavored additive to mimic taste and color. They should not use any form of oral hygiene during the study period. The solution contains no active anti-inflammatory ingredients. The placebo serves to determine baseline changes in plaque and gingivitis without active intervention.

Placebo Mouthwash

Pure Green Tea Mouthwash DRUG

Participants in this group will use a mouthwash prepared from pure green tea extract. The solution will be brewed by infusing 1 g of green tea leaves in 100 ml of boiling deionized water for 20 minutes, cooled, filtered, and stored in brown glass bottles. Participants will rinse with 10 ml of the mouthwash twice daily for 30 seconds over 3 weeks. They should not use any form of oral hygiene during the study period.

Pure Green Tea Mouthwash

Listerine Total Care Mouthwash DRUG

Participants in this group will use Listerine Total Care essential oils mouthwash, containing eucalyptol, menthol, methyl salicylate, and thymol. All participants will rinse with 10 ml twice daily for 30 seconds for 3 weeks. They should not use any form of oral hygiene during the study period. This will serve as an active control to compare the efficacy of essential oils with the pure green tea formulation and placebo.

Listerine Total Care Mouthwash

Listerine Green Tea Mouthwash DRUG

Participants will use Listerine Green Tea mouthwash, an essential oil-based product enhanced with green tea extract and fluoride. All participants will rinse with 10 ml twice daily for 30 seconds over 3 weeks. They should not use any form of oral hygiene during the study period. This will allow comparison between a commercial green tea-containing mouthwash and the pure green tea solution.

Listerine Green Tea Mouthwash

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Non-smokers
• Systemically healthy
• At least 24 teeth in the functional dentition, excluding third molars
• Clinical diagnosis of periodontal health (no periodontal pockets \> 3 mm, bleeding on probing percentage \< 10%, and no attachment loss).

You may not qualify if:

• Smokers.
• Medically compromised patients.
• Pregnant woman.
• Allergies to green tea or EO.
• Any condition that might affect the periodontal tissues.
• Patients with any carious lesions (initial or active).
• Patients who have overhang restorations, poorly designed crowns or bridges, and ortho retainers.
• Use of systemic antibiotics or anti-inflammatory medications within 3 months prior to enrolment.

Sponsors & Collaborators

• King Abdullah University Hospital: lead
• Jordan University of Science and Technology: collaborator

Study Sites (1)

Jordan University of Science and Technology, Faculty of Dentistry

Irbid, Irbid Governorate, 22110, Jordan

RECRUITING

MeSH Terms

Conditions

Dental Plaque; Gingivitis; Hemorrhage; Gingival Hemorrhage

Condition Hierarchy (Ancestors)

Dental Deposits; Tooth Diseases; Stomatognathic Diseases; Infections; Gingival Diseases; Periodontal Diseases; Mouth Diseases; Pathologic Processes; Pathological Conditions, Signs and Symptoms; Oral Hemorrhage

Study Officials

• Lana Bader, DClinDent Perio

  Jordan University of Science and Technology

  STUDY DIRECTOR

• Dana Abdullah, MClinDent Perio

  Jordan University of Science and Technology

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Lana Bader, DClinDent Perio

CONTACT

+962791200956; lmbader4@just.edu.jo

Dana Adel Abdullah, MClinDent Perio

CONTACT

+962796653746; lana.bader@yahoo.com

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: QUADRUPLE
• Who Masked: PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
• Masking Details: Participants, investigators, and outcome assessors will be blinded. Mouthwashs will be emptied in opaque bottles. They will be coded and unlabeled. A trained dental assisstant will be responsable about disposing the assigned mouth wash to the patient.
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Dr, Assistant Professor

Model Details: This is a double-blinded, parallel group, randomised, controlled, clinical trial with four arms: pure green tea mouthwash, Listerine Total Care, Listerine Green Tea, and placebo. Participants will rinse twice daily for three weeks. They will be prohibited from all forms of mechanical plaque control during the study period.

Study Record Dates

• First Submitted: December 29, 2025
• First Posted: January 23, 2026
• Study Start: December 1, 2025
• Primary Completion (Estimated): December 1, 2026
• Study Completion (Estimated): December 1, 2026
• Last Updated: January 23, 2026

Record last verified: 2026-01

Data Sharing

• IPD Sharing: Will share
• Shared Documents: STUDY PROTOCOL, SAP, ICF, CSR
• Time Frame: Individual Participant Data (IPD) and supporting documents (Study Protocol, Statistical Analysis Plan, Informed Consent Form, and Clinical Study Report) will be made available after publication of the study results, approximately one year after study completion.
• Access Criteria: Access will be granted to qualified researchers who request the data via email to the principal investigator. Researchers must sign a data use agreement ensuring ethical use, confidentiality, and prohibition of attempts to identify participants.

Locations

JO (1)