The Aim of the Study is to Check Whether Follicular Fluid Evaluation Using a Spectrophotometer Can Differentiate High Quality Oocytes From Low Quality Oocytes. Oocyte Quality Will be Defined Using GDF9, BMP15 and Oocyte Morphology.

NCT07329998 | Active Not Recruiting

• 1 other identifier: 0069-23-HYMC
• Study Type: interventional
• Target: 200
• Locations: 1 country
• Sites: 1

Brief Summary

The aim of the study is to check whether follicular fluid evaluation using a spectrophotometer can differentiate high quality oocytes from low quality oocytes. Oocyte quality will be defined using GDF9, BMP15 and oocyte morphology. The correlation between cortisol levels in women and oocyte quality parameters will be estimated.

Conditions

Oocyte Quality Assessment Using Spectrophotometer

Outcome Measures

Primary Outcomes (1)

• Difference in follicular fluid infrared spectrophotometric profiles between high-quality and low-quality oocytes.

  Infrared spectrophotometric measurements of follicular fluid collected at oocyte retrieval will be obtained using attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR). Oocytes will be classified as high-quality or low-quality based on predefined morphological criteria and follicular fluid levels of GDF9 and BMP15 measured by ELISA. The primary outcome will be reported as differences in normalized spectral absorbance features between the two oocyte quality groups, including discrimination performance using multivariate analysis.

  3 Years

Study Arms (1)

follicular fluid evaluation using spectrophotometer to differentiate high low quality oocytes

EXPERIMENTAL

Procedure: Oocyte quality assessment using spectrophotometer

Interventions

Oocyte quality assessment using spectrophotometer PROCEDURE

On the OPU (ovum pick-up) day the following blood biochemical markers will be measured as well- Blood interleukins 1-6, cytokine, hormone profile, lipid profile, cortisol. After collecting the oocytes- the Follicular fluid will be sent for the following tests- 1. mid-infrared spectrometry, 2. P, E2, testosterone, cortisol, cytokines, IL 1-6, lipids, fatty acids. 3. BMP-15, GDF-9 and HSPG2 4. GCMS-Gas Chromatography Mass Spectrophotometer

follicular fluid evaluation using spectrophotometer to differentiate high low quality oocytes

Eligibility Criteria

• Sex: female
• Healthy Volunteers: No
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)

You may qualify if:

• Women aged 20-40 years.
• Undergoing IVF or IVF-ICSI treatment.
• At least five follicles visualized on ultrasound at the time of oocyte retrieval
• Ability to provide written informed consent.

You may not qualify if:

• Age \>40 years.
• Fewer than five follicles at the time of oocyte retrieval.
• Use of donor oocytes.
• Significant medical comorbidities affecting ovarian function.
• Known ovarian malignancy or prior ovarian surgery significantly affecting follicular integrity.

Sponsors & Collaborators

• Hillel Yaffe Medical Center: lead

Study Sites (1)

Hille Yaffe Medical Center

Hadera, Israel, 3881000, Israel

Location

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: SCREENING
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER GOV
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: November 16, 2025
• First Posted: January 9, 2026
• Study Start: September 22, 2024
• Primary Completion: December 16, 2025
• Study Completion (Estimated): November 1, 2026
• Last Updated: January 9, 2026

Record last verified: 2026-01

Locations

IL (1)