Determination of RNA/DNA Damage Associated With Oxidative Stress in Periodontitis Patients

NCT07279896 | Completed

• 1 other identifier: ZH-2025-OxidativeStress
• Study Type: observational
• Target: 88
• Locations: 1 country
• Sites: 1

Brief Summary

This study looked at whether a type of gum disease called Stage III periodontitis and cigarette smoking are linked to higher levels of "oxidative stress." Oxidative stress can damage DNA and RNA, which are important parts of our cells. Two markers in saliva-8-OHdG (a sign of DNA damage) and 8-OHG (a sign of RNA damage)-were measured to understand this. Researchers included four groups of adults: smokers with periodontitis, non-smokers with periodontitis, healthy smokers, and healthy non-smokers. All participants had a dental exam, which checked things like gum inflammation, bleeding, and pocket depth. After the exam, an unstimulated saliva sample was collected from each person. The samples were tested in a laboratory using a sensitive method that measures oxidative damage. The study found that people with periodontitis had higher levels of DNA and RNA damage in their saliva than healthy people. The highest levels were seen in smokers with periodontitis. Even healthy smokers showed higher levels of oxidative stress than healthy non-smokers. In general, worse gum disease was linked to higher levels of oxidative damage. These results suggest that saliva tests for 8-OHdG and 8-OHG may help identify gum disease in a simple, non-invasive way. The findings also show that smoking adds to the body's oxidative stress, especially when gum disease is present. The study provides new information about how smoking and gum disease together may affect cell health.

Conditions

Periodontitis (Stage 3); Smoking

Keywords

Periodontitis; Smoking; Oxidative Stress; 8-OHdG; 8-OHG; Saliva; Biomarkers

Outcome Measures

Primary Outcomes (1)

• Salivary oxidative DNA and RNA damage (8-OHdG and 8-OHG levels)

  Concentrations of 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 8-hydroxyguanosine (8-OHG) measured in unstimulated whole saliva using a high-sensitivity competitive ELISA kit. Saliva samples are collected at a separate baseline visit within the study period, after full-mouth periodontal examination and before any periodontal therapy. Biomarker levels (ng/mL) were compared across the four study groups and analyzed in relation to clinical periodontal parameters. Higher values indicate greater oxidative nucleic acid damage.

  Baseline (periodontal examination visit and saliva collection visit, before any periodontal treatment)

Study Arms (4)

Periodontitis Smoker

Participants diagnosed with Stage III periodontitis who are current cigarette smokers. No intervention was applied; biological samples (unstimulated saliva) were collected for biomarker analysis.

Periodontitis Non-Smoker

Participants diagnosed with Stage III periodontitis who do not smoke. No intervention was applied; unstimulated saliva samples were collected for laboratory analysis.

Healthy Smoker

Periodontally healthy individuals who are current smokers. No treatment or intervention was performed; only saliva samples and periodontal measurements were obtained.

Healthy Non-Smoker

Periodontally healthy non-smokers. No intervention was provided; participants underwent periodontal evaluation and saliva sample collection.

Eligibility Criteria

• Age: 18 Years - 65 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

The study population consisted of systemically healthy adults aged 18-65 years, including individuals with Stage III periodontitis and periodontally healthy controls. Participants were categorized into four groups based on periodontal status and smoking habits: smokers and non-smokers with Stage III periodontitis, and smokers and non-smokers who were periodontally healthy.

You may qualify if:

• Individuals aged 18 years or older
• Having at least 16 permanent teeth, excluding third molars
• Not using orthodontic appliances
• Not pregnant or breastfeeding
• Systemically healthy individuals with no systemic diseases that could affect periodontal status
• No use of anti-inflammatory or antimicrobial medications within the last 6 months
• No periodontal treatment within the last 12 months

You may not qualify if:

• Pregnant or breastfeeding individuals.
• Use of anti-inflammatory or antimicrobial drugs in the past 6 months.
• Use of antioxidant supplements in the past 3 months.
• Periodontal treatment within the past 12 months.
• Diagnosis of psychiatric disorders.
• Any oral infection.
• Fewer than 16 permanent teeth (excluding third molars).
• Alcohol dependence.
• Active infectious diseases (Acute hepatitis, AIDS, tuberculosis), malignancy, or any systemic condition that may affect periodontal tissues.
• Current use of medications known to affect periodontal tissues (phenytoin, cyclosporine A, calcium channel blockers).

Sponsors & Collaborators

• Zehra Hasgül: lead

Study Sites (1)

Ankara University Faculty of Dentistry, Department of Periodontology

Ankara, Yenimahalle, 06560, Turkey (Türkiye)

Location

Related Publications (3)

• Hendek MK, Erdemir EO, Kisa U, Ozcan G. Effect of initial periodontal therapy on oxidative stress markers in gingival crevicular fluid, saliva, and serum in smokers and non-smokers with chronic periodontitis. J Periodontol. 2015 Feb;86(2):273-82. doi: 10.1902/jop.2014.140338. Epub 2014 Oct 17.

  PMID: 25325515 RESULT

• Tonetti MS, Greenwell H, Kornman KS. Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. J Periodontol. 2018 Jun;89 Suppl 1:S159-S172. doi: 10.1002/JPER.18-0006.

  PMID: 29926952 RESULT

• Kurgan S, Onder C, Altingoz SM, Bagis N, Uyanik M, Serdar MA, Kantarci A. High sensitivity detection of salivary 8-hydroxy deoxyguanosine levels in patients with chronic periodontitis. J Periodontal Res. 2015 Dec;50(6):766-74. doi: 10.1111/jre.12263. Epub 2015 Feb 7.

  PMID: 25662588 RESULT

Biospecimen

Retention: SAMPLES WITH DNA

Unstimulated whole saliva samples stored at -80°C. Saliva samples may contain cellular material and DNA.

MeSH Terms

Conditions

Periodontitis; Smoking

Condition Hierarchy (Ancestors)

Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases; Behavior

Study Officials

• Sivge Kurgan

  Ankara University, Faculty of Dentistry, Department of Periodontology

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Periodontology Specialist (Resident)

Study Record Dates

• First Submitted: December 1, 2025
• First Posted: December 12, 2025
• Study Start: May 5, 2025
• Primary Completion: August 4, 2025
• Study Completion: August 15, 2025
• Last Updated: December 12, 2025

Record last verified: 2025-12

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)