NCT07246486

Brief Summary

Up to 50% of all women suffer of a spontaneous abortion. Having two/three or more spontaneous abortions is one of the main reasons of unwanted childlessness. It is well known that the intestinal microbiome has many important roles in a healthy organism. Simultaneously, a dysbiosis can lead to diseases like, for example, chronic inflammatory bowel diseases, autoimmune diseases or diabetes. Several studies showed that there is a connection between a vaginal dysbiosis and premature birth or late abortion. It is still unclear what effects the composition of the intestinal microbiome before pregnancy has on the course of pregnancy. This study will be investigate if there is a connection between the composition of the intestinal microbiome and the occurence of recurrent spontaneous abortions.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
60

participants targeted

Target at P25-P50 for all trials

Timeline
Completed

Started Nov 2020

Typical duration for all trials

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

November 1, 2020

Completed
2.3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

February 28, 2023

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

February 28, 2023

Completed
2.7 years until next milestone

First Submitted

Initial submission to the registry

November 17, 2025

Completed
7 days until next milestone

First Posted

Study publicly available on registry

November 24, 2025

Completed
Last Updated

November 24, 2025

Status Verified

November 1, 2020

Enrollment Period

2.3 years

First QC Date

November 17, 2025

Last Update Submit

November 17, 2025

Conditions

Keywords

microbiome, spontaneous abortion

Outcome Measures

Primary Outcomes (3)

  • Intestinal microbiome

    Microbiome Analysis from the stool samples

    48 month

  • Hormon levels

    Hormone levels of Estrogen, Progesterone from blood and urine samples

    48 month

  • bacterial metabolic products

    Metabolic products phytoestrogene enterodiol, enterolactone from blood and urine samples

    48 month

Study Arms (3)

2 spontaneous abortions

non-pregnant women with two spontaneous abortions in medical history

3 spontaneos abortions

non-pregnant women with two spontaneous abortions in medical history

controle-group

non-pregnant women with at least one complication-free pregnancy with no premature birth or late abortion in medical history

Eligibility Criteria

Age18 Years - 40 Years
Sexfemale
Age GroupsAdult (18-64)
Sampling MethodNon-Probability Sample
Study Population

non-pregnant women between 18 and 40 years

You may qualify if:

  • Patient of the fertility center of the university hospital Tübingen
  • or 3 spontaneous abortions in medical history

You may not qualify if:

  • Age under 18 years, age over 40 years
  • Current pregnancy
  • Immunosuppressive therapy
  • Antibiotic therapy up to four weeks before admission
  • Acute infection
  • Orale intake of probiotics up to four weeks before admission

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

University Hospital Tübingen

Tübingen, Baden-Wurttemberg, 72076, Germany

Location

Biospecimen

Retention: SAMPLES WITH DNA

Stool samples for microbiome analyses Bloodserum and urine for hormone levels for bacterial metabolic products.

MeSH Terms

Conditions

Abortion, Spontaneous

Condition Hierarchy (Ancestors)

Pregnancy ComplicationsFemale Urogenital Diseases and Pregnancy ComplicationsUrogenital Diseases

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR INVESTIGATOR
PI Title
PD Dr. med. Natascha Köstlin-Gille

Study Record Dates

First Submitted

November 17, 2025

First Posted

November 24, 2025

Study Start

November 1, 2020

Primary Completion

February 28, 2023

Study Completion

February 28, 2023

Last Updated

November 24, 2025

Record last verified: 2020-11

Locations