Examination of the Effect of Nd:YAG Laser on Oxidative Stress and Matrix Metalloproteinase-9 in Teeth With Apical Periodontitis

NCT07243353 | Completed

• 1 other identifier: 2024/046
• Study Type: observational
• Target: 58
• Locations: 1 country
• Sites: 1

Brief Summary

The objective of this clinical investigation is to examine the impact of Nd:YAG laser therapy on oxidative stress and matrix metalloproteinase-9 (MMP-9) levels in teeth afflicted with apical periodontitis. Samples were collected from patients who had undergone treatment with the Nd:YAG laser and compared with samples collected from patients who had not received this treatment. The study commenced with a sample of 60 patients. However, the study was completed with 58 patients. The study concluded with the following finding: the use of Nd:YAG lasers was not found to be superior.

Conditions

Apical Periodontitis; Oxidative Stress

Keywords

Apical periodontitis; Matrix metalloproteinase-9; Nd:YAG laser; Oxidative stress

Outcome Measures

Primary Outcomes (1)

• Measurement of TOS, TAOC and MMP-9 levels in periapical exudate and GCF

  TOS, TAOC and MMP-9 levels were measured by ELISA in periapical exudate and GCF samples collected during routine root canal treatment in patients with apical periodontitis. No significant difference was detected in the change in biomarker levels between the group treated with the Nd:YAG laser and the control group.

  The initial and seventh days of the treatment period

Secondary Outcomes (1)

• Determination of the correlation relationship between TOS, TAOC and MMP-9 levels in periapical exudate and GCF samples

  The initial and seventh days of the treatment period

Study Arms (2)

Nd:YAG laser group

Group treated with Nd:YAG laser

Control group

Group not treated with Nd:YAG laser

Eligibility Criteria

• Age: 25 Years - 50 Years
• Gender Eligibility Details: biologically male and female patients
• Healthy Volunteers: No
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

The study groups comprised of patients who had applied to the Department of Endodontics at the Faculty of Dentistry at Kırıkkale University and that underwent endodontic treatment from 15 February 2024 to 15 October 2024.

You may qualify if:

• Patients aged between 25 and 50 years and classified as American Society of Anesthesiologists (ASA) I. The maxillar and mandibular incisors and premolars with chronic apical periodontitis of a PAI score of 3 or 4 were included.

You may not qualify if:

• Patients with American Society of Anesthesiologists (ASA) classification II or higher, pregnant, patients with localised or generalised periodontitis, patients who had used antibiotics in the last month and anti-inflammatory drugs in the last week, smokers, and teeth with internal or external root resorption, swelling, palpation pain or fistula in the relevant area, root fracture or incomplete root formation, and teeth that could not be isolated with rubber dam were not included in the study.

Sponsors & Collaborators

• Kırıkkale University: lead

Study Sites (1)

Kırıkkale Üniversitesi Diş Hekimliği Fakültesi

Kırıkkale, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Periapical Periodontitis

Condition Hierarchy (Ancestors)

Periapical Diseases; Jaw Diseases; Stomatognathic Diseases; Periodontal Diseases; Mouth Diseases; Periodontitis

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: November 17, 2025
• First Posted: November 21, 2025
• Study Start: February 1, 2024
• Primary Completion: November 1, 2024
• Study Completion: November 8, 2024
• Last Updated: December 15, 2025

Record last verified: 2025-11

Locations

TU (1)