Antibacterial Effect of Ozonated Olive Oil, Propolis and Chlorhexidine Mouthwashes in Pediatric Patients

NCT07197073 | Completed

• 1 other identifier: 0937_07/2024
• Study Type: interventional
• Target: 51
• Locations: 1 country
• Sites: 1

Brief Summary

Streptococcus mutans and Lactobacillus acidophilus are the most common cariogenic microorganisms. Chlorhexidine has been the most widely used antibacterial mouthwash in dentistry. On the other hand, few studies have reported the antibacterial potential of Propolis and Ozonated olive oil as antibacterial agents that can be used as alternatives to Chlorhexidine. This study aims to evaluate and compare the antibacterial effect of Ozonated olive oil, Propolis, and Chlorhexidine on S. mutans and L. acidophilus count in pediatric patients.

Conditions

Oral Hygiene; Oral Health

Keywords

Ozonated olive oil; Chlorhexidine; Antibacterial; Propolis

Outcome Measures

Primary Outcomes (2)

• Change in microbial count

  Total bacterial count before and after the intervention was detected. plaque samples were dispersed by vortexing for 30 seconds then 10 fold serially diluted using sterile saline.

  Baseline and after 14 days of treatment

• Change in plaque accumulation

  The plaque accumulation was scored for each tooth on its four surfaces.The number of positively scored surfaces is divided by the total number of tooth surfaces evaluated and the result is multiplied by 100 to express the index as a percentage

  Baseline and after 14 days of treatment

Study Arms (3)

Ozonated olive oil mouthwash

EXPERIMENTAL

Other: Ozonated olive oil

Propolis mouthwash

EXPERIMENTAL

Other: Propolis

Chlorhexidine mouthwash

ACTIVE COMPARATOR

Other: Chlorhexidine

Interventions

Ozonated olive oil OTHER

Ozonated olive oil mouthwash will be prepared by Smart Ozone Marvel using by company's ozone. Children will be instructed to use 10 ml of mouthwash for 1 minute twice daily after 1 hour after tooth brushing

Ozonated olive oil mouthwash

Propolis OTHER

Propolis mouthwash will be prepared by using 2% raw propolis, 40 ml flavouring and 0.1 gm coloring agents, 150 ml propylene glycol, and 60 gm sorbitol. Children will be instructed to use 10 ml of mouthwash for 1 minute twice daily after 1 hour after tooth brushing

Propolis mouthwash

Chlorhexidine OTHER

The patients were given 0.12% Chlorhexidine. Children will be instructed to use 10 ml of mouthwash for 1 minute twice daily after 1 hour after tooth brushing

Chlorhexidine mouthwash

Eligibility Criteria

• Age: 8 Years - 12 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17)

You may qualify if:

• High caries risk patients, according to AAPD guidelines, with visible caries or radiographic penetration into dentin, white spots on smooth surfaces, or recent restorations or missing teeth due to caries.
• Cooperative children according to Frankl's rating (score 3 \& 4)

You may not qualify if:

• Children with any medical illness or oral infection or those who received any type of antibiotic treatment or mouthwash for two weeks before or during the study were excluded

Sponsors & Collaborators

• Alexandria University: lead

Study Sites (1)

Outpatient Clinic of Pediatric dentistry, Faculty of dentistry, Alexandria University, Egypt

Alexandria, Egypt

Location

MeSH Terms

Interventions

Propolis; Chlorhexidine

Intervention Hierarchy (Ancestors)

Resins, Plant; Biopolymers; Polymers; Macromolecular Substances; Plant Exudates; Biological Products; Complex Mixtures; Biguanides; Guanidines; Amidines; Organic Chemicals

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: OUTCOMES ASSESSOR
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: September 21, 2025
• First Posted: September 29, 2025
• Study Start: February 25, 2025
• Primary Completion: May 10, 2025
• Study Completion: May 10, 2025
• Last Updated: September 29, 2025

Record last verified: 2025-09

Data Sharing

• IPD Sharing: Will not share

Locations

EG (1)