Impact of Chronic and Acute Caffeine Intake on the Hematopoietic System

NCT07193264 | Not Yet Recruiting

• 1 other identifier: 2025-00914
• Study Type: interventional
• Target: 100
• Locations: 1 country
• Sites: 1

Brief Summary

This study investigates how caffeine intake affects blood stem and progenitor cells in healthy adults. The trial will compare people who regularly consume caffeine with those who consume very little or none. All participants will receive a single 200 mg caffeine tablet (similar to one cup of coffee) under fasting conditions. Blood samples will be collected before and three hours after caffeine intake. The study will assess whether caffeine influences the mobilization of blood stem and progenitor cells from the bone marrow into the bloodstream. It will also examine the effects of caffeine on the function, gene activity, and metabolism of blood cells.

Conditions

Healthy Adults; Hematopoietic System; Caffeine

Keywords

Caffeine consumption; caffeine; Hematopoietic Stem Cells; Hematopoietic Stem and Progenitor Cells; Effect of caffeine on blood system; Effect of caffeine on hematopoietic system

Outcome Measures

Primary Outcomes (2)

• Mobilization of hematopoietic stem and progenitor cells

  The change in the numbers of mobilized HSPCs (defined as CD34+ cells) \[cells/ml\] in blood (measured by flow cytometry) from baseline to three hours after intake of a single oral dose of 200mg caffeine in habitual caffeine consumers versus non-caffeine consumers.

  From baseline to three hours after caffeine intake

• Mobilization of hematopoietic stem and progenitor cells

  The change in the relative abundance of HSPCs \[% HSPCs of total PBMCs\] in blood (measured by flow cytometry) from baseline to three hours after intake of a single oral dose of 200mg caffeine in habitual caffeine consumers versus non-caffeine consumers.

  From baseline to three hours after caffeine intake

Secondary Outcomes (6)

• Functional alterations in CD34+ peripheral blood cells

  From baseline to three hours after caffeine intake

• Functional alterations in CD34+ peripheral blood cells

  From baseline to three hours after caffeine intake

• Transcriptional changes in CD34+ cells

  From baseline to three hours after caffeine intake

• Relative metabolite abundance

  From baseline to three hours after caffeine intake

• Change in the numbers of peripheral blood cells

  From baseline to three hours after caffeine intake

Study Arms (1)

Caffeine intervention

EXPERIMENTAL

200mg Caffeine

Other: Caffeine (200 mg)

Interventions

Caffeine (200 mg) OTHER

Participants receive a single oral dose of 200 mg caffeine under fasting conditions. Blood samples are collected before and three hours after intake to evaluate effects on hematopoietic stem and progenitor cells, including mobilization, function, gene expression, and metabolism.

Caffeine intervention

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Able to give consent.
• Healthy adults aged ≥ 18 years.
• Regular caffeine consumers (≥1 coffee cup/day or ≥1 energy drink/day) or non-consumers (≤1 cup/month and ≤1 energy drink/month) for at least 6 months.
• Willingness to comply with fasting requirements and study procedures.

You may not qualify if:

• Chronic illnesses or medication use affecting metabolism, including metabolic disorders (e.g., diabetes, thyroid dysfunction, metabolic syndrome).
• Personal history of cancer.
• Personal history of hematological disorders.
• History of radiation therapy.
• Known caffeine hypersensitivity or intolerance.
• Known immunosuppression.

Sponsors & Collaborators

• Jasmin Rettkowski: lead
• ETH Zurich: collaborator
• Laboratory of Stem Cell Biology and Ageing, ETH Zurich, Prof. Nina Cabezas-Wallscheid: collaborator
• Prof. Dr. Nina Cabezas-Wallscheid, ETH Zurich: collaborator

Study Sites (1)

ETH GLC

Zurich, Canton of Zurich, 8006, Switzerland

Location

MeSH Terms

Interventions

Caffeine

Intervention Hierarchy (Ancestors)

Xanthines; Alkaloids; Heterocyclic Compounds; Purinones; Purines; Heterocyclic Compounds, 2-Ring; Heterocyclic Compounds, Fused-Ring

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Dr. Jasmin Rettkowski, Principal Investigator, Department of Health Sciences and Technology, ETH Zurich

Model Details: This is a single-group, open-label study in which all participants receive a single oral dose of 200 mg caffeine under fasting conditions. Blood samples are collected before and three hours after caffeine intake to assess mobilization, function, gene expression, and metabolic profile of hematopoietic stem and progenitor cells.

Study Record Dates

• First Submitted: September 18, 2025
• First Posted: September 25, 2025
• Study Start: October 1, 2025
• Primary Completion (Estimated): December 1, 2027
• Study Completion (Estimated): June 1, 2028
• Last Updated: September 29, 2025

Record last verified: 2025-09

Data Sharing

• IPD Sharing: Will share

Locations

CH (1)