Effects of Different Fluid Transfusion Regimens On Glycocalyx In Patients Undergoing Thoracic Surgery.

NCT07192523 | Completed

• 1 other identifier: 2019/514/154/10
• Study Type: interventional
• Target: 41
• Locations: 1 country
• Sites: 1

Brief Summary

Introduction: Endothelial Glycocalyx (EG) composing of proteoglycans heparan sulfate (HS), syndecan, and plasma proteins forms a physiologically active surface layer on the vascular endothelium, regulates oncotic pressure. We aimed to compare the effects of two different fluid transfusion regimens on EG. Materials and Methods: 41 patients with ASA I-III physical status were included in this prospective, randomized controlled study. We collected blood samples before anesthesia induction and after surgery to measure hemoglobin-hematocrit levels and biochemical parameters. Hemodynamic parameters were recorded every 15 minutes perioperatively. We divided the patients into two groups: Group R received 3 ml/kg/h, and Group L received 8 ml/kg/h of Ringer's lactate infusion. To assess endothelial damage, HS and syndecan levels, blood samples were taken after anesthesia induction(T1), after pneumonectomy or lobectomy(T2), and at the end of surgery(T3).

Conditions

Hypervolemia; Hypovolemia

Keywords

Endothelial Dysfunction

Outcome Measures

Primary Outcomes (1)

• Our primary aim in the study was to evaluate the changes in HS and syndecan 1 levels after 8 ml/kg/hr or 3 ml/kg/hr fluid transfusion in thoracic surgery.

  To evaluate endothelial damage, blood samples were taken from patients in both groups 3 times. The time of taking the samples was determined as; the blood sample taken after the placement of the IV cannula (T1), the blood sample taken after pneumonectomy or lobectomy (T2), and the blood sample taken after the patient was extubated following the end of surgery (T3). Venous blood samples were placed in a 5 ml vacuum tube with a serum separator. The samples were centrifuged at 1500 g for 10 minutes to separate the serum, and these samples were stored at -80 °C.

  6 month

Study Arms (2)

Effects of 3ml Fluid Transfusion Regimens On Glycocalyx

OTHER

In intraoperative fluid therapy, Group Restrictive received 3ml/kg/hr Ringer Lactate

Other: Restrictive fluid therapy

Effects of 8 ml Fluid Transfusion Regimens On Glycocalyx

OTHER

In intraoperative fluid therapy, one group Group Liberal received 8ml/kg/hr crystalloid Ringer Lactate.

Other: Liberal fluid therapy

Interventions

Restrictive fluid therapy OTHER

Group Restrictive (Group R) received 3ml/kg/hr, crystalloid (Ringer Lactate).

Also known as: 3 ml

Effects of 3ml Fluid Transfusion Regimens On Glycocalyx

Liberal fluid therapy OTHER

Group Restrictive liberal received 8ml/kg/hr, crystalloid (Ringer Lactate).

Also known as: 8 ml

Effects of 8 ml Fluid Transfusion Regimens On Glycocalyx

Eligibility Criteria

• Age: 18 Years - 75 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Being 18-65 years old and in the American Society of Anaesthesiologists (ASA) I-III risk group.

You may not qualify if:

• Having heart failure
• Having a previously known vascular disease

Sponsors & Collaborators

• Kocaeli Derince Education and Research Hospital: lead

Study Sites (1)

Kartal Dr Lutfi Kirdar City Hospital

Istanbul, Kartal, 34000, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Edema; Hypovolemia

Interventions

N-(2-aminophenyl)-6-(4-(2-(3,5-dihydroxycyclohexylidene)ethylidene)-7a-methyloctahydro-1H-inden-1-yl)-4-methylhepta-2,4-dienamide

Condition Hierarchy (Ancestors)

Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Pathologic Processes

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SEQUENTIAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: ASSOCİATED PROFESSOR

Study Record Dates

• First Submitted: June 10, 2025
• First Posted: September 25, 2025
• Study Start: August 15, 2021
• Primary Completion: December 15, 2021
• Study Completion: August 30, 2025
• Last Updated: January 5, 2026

Record last verified: 2026-01

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)