Inflammatory Responses After CrossFit and Resistance Trainings in Untrained Subjects

NCT07190924 | Completed DMC

• 1 other identifier: ISS-1386
• Study Type: interventional
• Target: 24
• Locations: 1 country
• Sites: 1

Brief Summary

Considering the remarkable increase of the fitness programs, the purpose of this study was to assess and compare the effect of Crossfit (CF) and periodized resistance (RT) trainings on inflammatory/oxidative responses in untrained individuals. Twenty-four participants (20 men and 4 women, age= 25.7 ± 2.2 years, BMI= 25.7 kg.m-2) were randomly assigned into 12 weeks of these trainings with a rate of five sessions/week. Before and after intervention, participants anthropometric data and graded exercise tests were performed. Before (pre), immediately after (post) and 60min after test (p60) a 20mL venous blood sample were drawn for a later measure of c-reactive protein (CRP), interleukin 1 beta (IL-1ß), tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), interleukin 10 (IL-10), thiobarbituric acid reactive substances (TBARS), antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPx).

Conditions

Inflammation; Oxidative Stress; Plasma Volume; Hormones

Outcome Measures

Primary Outcomes (8)

• Inflammatory marker

  c-reactive protein (CRP),

  Participants data were performed in one day before beginning the 12 week intervention and one day after the last session

• Inflammatory marker

  interleukin 1 beta (IL-1ß)

  Participants data were performed in one day before beginning the 12 week intervention and one day after the last session

• Inflammatory marker

  tumor necrosis factor alpha (TNF-α)

  Participants data were performed in one day before beginning the 12 week intervention and one day after the last session

• Inflammatory marker

  interleukin 6 (IL-6)

  Participants data were performed in one day before beginning the 12 week intervention and one day after the last session

• Inflammatory marker

  interleukin 10 (IL-10)

  Participants data were performed in one day before beginning the 12 week intervention and one day after the last session

• Oxidative stress

  thiobarbituric acid reactive substances (TBARS)

  Participants data were performed in one day before beginning the 12 week intervention and one day after the last session

• Oxidative stress

  antioxidant enzymes catalase (CAT)

  Participants data were performed in one day before beginning the 12 week intervention and one day after the last session

• Oxidative stress

  glutathione peroxidase (GPx)

  Participants data were performed in one day before beginning the 12 week intervention and one day after the last session

Study Arms (2)

CrossFit

EXPERIMENTAL

High intensity functional training

Behavioral: 12 week training

Resistance

EXPERIMENTAL

Periodized resistance training

Behavioral: 12 week training

Interventions

12 week training BEHAVIORAL

Anti-inflammatory and oxidative environment following 12-weeks of CF training by evaluating inflammatory markers and oxidative precursors in untrained participants

CrossFit

Eligibility Criteria

• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)

You may qualify if:

• Participants must be non-smokers,
• Participants should not suffer from respiratory issues
• Participants should have healthy kidney and liver
• Participants have no metabolic and neurological disorders

You may not qualify if:

• The use of medications during the previous 4 weeks
• Consumption of any product related to anti-inflammatory/oxidative supplements

Sponsors & Collaborators

• Habil Hamdouni: lead

Study Sites (1)

Institut Supérieur du Sport et de l'Education Physique de Ksar-Saïd

Aryanah, Ariana Governorate, 2073, Tunisia

Location

MeSH Terms

Conditions

Inflammation

Condition Hierarchy (Ancestors)

Pathologic Processes; Pathological Conditions, Signs and Symptoms

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Doctor

Study Record Dates

• First Submitted: September 14, 2025
• First Posted: September 24, 2025
• Study Start: November 8, 2021
• Primary Completion: February 8, 2022
• Study Completion: April 17, 2022
• Last Updated: September 24, 2025

Record last verified: 2025-09

Locations

TU (1)