Evaluation of the Ergogenic Properties of Hesperetin on Exercise-induced Fatigue

NCT07104734 | Completed

• 1 other identifier: CMUH113-REC2-012
• Study Type: interventional
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

Twenty young, healthy, physically active men and women (12\~30 years) did a pre-post trial randomized into placebo or HST trials (500 mg/d for two weeks). HST supplementation enhanced endurance judo-specific performance and effectively attenuated exercise-induced fatigue, oxidative stress, inflammatory response, and muscle damage.

Conditions

Healthy; Athletes

Keywords

Hesperetin; judo; exercise performance

Outcome Measures

Primary Outcomes (10)

• Special Judo Fitness Test (SJFT)

  The Special Judo Fitness Test (SJFT) was developed by Sterkowicz (1995) and has been validated through multiple studies demonstrating its objectivity, reliability, and validity. The SJFT consists of a task in which the athlete must throw a partner as many times as possible using maximum effort. The test is divided into three intervals: 15 seconds, 30 seconds, and 30 seconds, with 10-second rest periods in between. During each interval, the subject is verbally encouraged to perform as many throws as possible using the ippon-seoi-nage technique, with the throwing partners positioned 6 meters apart. After the test, heart rate (HR) is recorded immediately and again one minute post-exercise. The SJFT index is calculated based on the total number of throws and heart rate data using the following formula: SJFT Index = (Final HR \[bpm\] + HR at 1 minute post-test \[bpm\] / Total Number of Throws.

  Including for 2 days in both pre-post test trials (which lasts around 2 weeks).

• Upper Body Grip Strength Test

  Grip strength will be measured using a handgrip dynamometer (SHM-18) to assess upper limb muscle strength. First, the examiner instructs the participant to let both arms hang naturally by the sides of the body. The grip span of the dynamometer is then adjusted so that the second knuckle of the index finger forms a right angle when gripping the handle. Once the correct posture is confirmed, the test begins.Grip strength will be measured on both the dominant and non-dominant hands. After completing one side and recording the value, the device is reset to zero, and the participant rests for 30 seconds before testing the other hand. During the test, the dynamometer must not come into contact with the body.Each hand is tested twice, and the maximum value (measured in kilograms) from the two trials on each side is recorded as the reference score.

  Including for 2 days in both pre-post test trials (which lasts around 2 weeks).

• Lower Limb Explosive Power - Countermovement Jump (CMJ)

  The CMJ is used to assess lower limb maximal strength, explosive power, and speed. The CMJ test will be conducted using a sports performance measurement system to evaluate participants' lower limb explosive power. Under the guidance of the tester, participants will stand upright with knees fully extended, feet approximately shoulder-width apart, and hands placed on their waist to restrict arm swing, isolating lower limb performance.Participants will perform two maximal-effort jumps, with a 1-minute rest between attempts. The best jump height will be recorded as the test result. During the test, upon the tester's "start" command, participants will perform a rapid downward (countermovement) action to a self-selected depth, immediately followed by a maximal vertical jump.

  Including for 2 days in both pre-post test trials (which lasts around 2 weeks).

• 10-Second Anaerobic Power Test

  The test will be conducted using a stationary Monark 894E cycle ergometer. During the recovery period after each simulated judo match, participants will begin pedaling at a cadence of 60 rpm. The tester will then count down "3, 2, 1," and upon the "Go" command, participants will pedal at maximal effort and speed for 10 seconds. After completing the 10-second sprint, participants will continue pedaling at 60 rpm for a cooldown period as instructed by the tester to prevent dizziness caused by sudden cessation of exercise.Each participant will complete one 10-second anaerobic power test during the recovery period of each judo match. Anaerobic power indicators include peak power (occurring within the first 3 to 5 seconds of the test), total power output (W), and relative power output normalized to body weight (W/kg). The resistance load for the Wingate anaerobic power test is set at body weight (kg) × 0.75.

  Including for 2 days in both pre-post test trials (which lasts around 2 weeks).

• Assessment of the profile of mood state (POMS)

  POMS brief was chosen for this study to evaluate the mood of individuals. After the TTE, participants completed the form, and their responses were analyzed using simple statistics. The results were shown in six dimensions, one positive: (1) vigor; and five negatives: (2) tension, (3) depression, (4) anger, (5) fatigue and (6) confusion.

  Including for 2 days in both pre-post test trials (which lasts around 2 weeks).

• Clinical Biochemistry of muscle damage biomarkers

  Blood samples were collected at five different time points (15 min before the beginning (S), after each match (Match 1\~3), and immediately (E) after the Special Judo Fitness Test. The concentrations of muscle damage markers creatine kinase (CK), myoglobin, and high-sensitivity C-reactive protein (hs-CRP) were measured using commercial assay kits (San Diego, CA, USA) and enzyme-linked immunosorbent assay (ELISA) with a microplate reader (Tecan GENios, A-5082, Austria). Absorbance was measured at 450 nm, 505 nm, and 572 nm, respectively. The concentrations of CK, myoglobin, and hs-CRP in serum samples were determined based on standard curves.

  Including for 2 days in both pre-post test trials (which lasts around 2 weeks).

• Clinical Biochemistry of oxidative stress

  Blood samples were collected at five different time points (15 min before the beginning (S), after each match (Match 1\~3), and immediately (E) after the Special Judo Fitness Test. The oxidative stress markers, including total antioxidant capacity (TAC), malondialdehyde (MDA), and thiobarbituric acid reactive substances (TBARS), were measured using commercial assay kits (Sigma, St.) and enzyme-linked immunosorbent assay (ELISA) with a microplate reader (Tecan GENios, A-5082, Austria). Absorbance was measured at 532 nm and 570 nm, and the concentrations of TAC, MDA, and TBARS in serum samples were determined based on standard curves.

  Including for 2 days in both pre-post test trials (which lasts around 2 weeks).

• Clinical Biochemistry of metabolic markers and safety assessment

  Blood samples were collected at five different time points (15 min before the beginning (S), after each match (Match 1\~3), and immediately (E) after the Special Judo Fitness Test. The metabolic markers glucose, non-esterified fatty acid (NEFA), and triglycerides (TG) were analyzed using commercial assay kits (WAKO NEFA, Germany) and measured with a fully automated biochemical analyzer (7020, Hitachi Science Systems, Ltd., Ibaraki, Japan). For safety assessment, GOP and GPT levels were determined using enzymatic analysis with commercial assay kits (Sigma, St.) and enzyme-linked immunosorbent assay (ELISA) on a microplate reader (Tecan GENios, A-5082, Austria). Absorbance was measured at 595 nm, and the concentrations of GOP and GPT in serum samples were calculated based on standard curves.

  Including for 2 days in both pre-post test trials (which lasts around 2 weeks).

• Clinical Biochemistry of cytokines

  Blood samples were collected at five different time points (15 min before the beginning (S), after each match (Match 1\~3), and immediately (E) after the Special Judo Fitness Test. The cytokines interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were measured using commercial assay kits (Bio-Legend Inc., No. 430207, San Diego, CA, USA) and enzyme-linked immunosorbent assay (ELISA) with a microplate reader (Tecan GENios, A-5082, Austria). Absorbance was read at wavelengths of 450 nm and 570 nm, and the concentrations of IL-6 and TNF-α in serum samples were determined based on standard curves.

  Including for 2 days in both pre-post test trials (which lasts around 2 weeks).

• Clinical Biochemistry of stress markers

  Blood samples were collected at five different time points (15 min before the beginning (S), after each match (Match 1\~3), and immediately (E) after the Special Judo Fitness Test. The stress markers catecholamine, cortisol, and testosterone were analyzed using commercial assay kits (San Diego, CA, USA) and measured with an enzyme-linked immunosorbent assay (ELISA) reader (Tecan GENios, A-5082, Austria). Absorbance was measured at 450 nm, 505 nm, and 572 nm, respectively. The concentrations of catecholamine, cortisol, and testosterone in serum samples were determined based on standard curves.

  Including for 2 days in both pre-post test trials (which lasts around 2 weeks).

Study Arms (2)

Placebo

PLACEBO COMPARATOR

Containing edible yellow No. 4, edible yellow No. 5, sucrose, silica, talc, oxidized starch, gelatin, magnesium stearate, and palm wax.

Other: Placebo (Placebo trial); Other: Hesperetin (Hesperetin trial)

Hesperetin

EXPERIMENTAL

Each capsule containing 250 mg of Hesperetin

Other: Placebo (Placebo trial); Other: Hesperetin (Hesperetin trial)

Interventions

Placebo (Placebo trial) OTHER

The Placebo capsule supplement was taken for 2 weeks (2 capsules per day)

Hesperetin; Placebo

Hesperetin (Hesperetin trial) OTHER

The Hesperetin capsule supplement was taken for 2 weeks, with a daily dosage of 500 mg of Hesperetin (equivalent to 2 capsules per day, each containing 250 mg of Hesperetin and 50 mg Pycnogenol®).

Hesperetin; Placebo

Eligibility Criteria

• Age: 12 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64)

You may qualify if:

• Judo athletes aged 12 to 30 years with at least 5 years of judo training experience.

You may not qualify if:

• All participants must be free from chronic conditions such as heart disease and hypertension, and must not have experienced any serious lower limb musculoskeletal injuries within the past six months.

Sponsors & Collaborators

• China Medical University Hospital: lead
• China Medical University, Taiwan: collaborator

Study Sites (1)

China Medical University

Taichung, Taiwan

Location

MeSH Terms

Interventions

hesperetin

Study Officials

• Jung-Piao Tsao

  China Medical University, China

  STUDY CHAIR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: QUADRUPLE
• Who Masked: PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
• Purpose: OTHER
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: This study recruited 20 healthy male and female participants aged 12 to 30 years with at least 5 years of judo training experience to participate in a single-blind, pre-post test trial. Participants will be randomly assigned to either the hesperetin (Hst) group or the placebo group. All participants must be free from chronic conditions such as heart disease and hypertension, and must not have experienced any serious lower limb musculoskeletal injuries within the past six months. Throughout the study period, participants will be required to maintain their regular daily routines and will be prohibited from consuming any other nutritional supplements or alcohol.

Study Record Dates

• First Submitted: July 29, 2025
• First Posted: August 5, 2025
• Study Start: September 3, 2024
• Primary Completion: June 30, 2025
• Study Completion: June 30, 2025
• Last Updated: August 5, 2025

Record last verified: 2025-07

Data Sharing

• IPD Sharing: Will not share

Locations

TW (1)