NCT07085845

Brief Summary

The investigators will test the cytological performance of a nylon-flocked swab at two insertion depths in the anus, 3 cm and 5 cm, to determine which insertion depth is better at collecting cells from the squamocolumnar junction (SCJ), an anatomic site that is particularly vulnerable to carcinogenic transformation. The investigators hypothesize that the 5 cm insertion will result in a higher quality specimen with cells from the SCJ. However, the deeper swab insertion may affect end user acceptability without increasing adequacy or quality.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
50

participants targeted

Target at P25-P50 for not_applicable

Timeline
Completed

Started Jul 2025

Shorter than P25 for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

July 10, 2025

Completed
15 days until next milestone

First Posted

Study publicly available on registry

July 25, 2025

Completed
6 days until next milestone

Study Start

First participant enrolled

July 31, 2025

Completed
1 month until next milestone

Primary Completion

Last participant's last visit for primary outcome

September 12, 2025

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

September 12, 2025

Completed
2 months until next milestone

Results Posted

Study results publicly available

November 18, 2025

Completed
Last Updated

January 27, 2026

Status Verified

December 1, 2025

Enrollment Period

1 month

First QC Date

July 10, 2025

Results QC Date

November 4, 2025

Last Update Submit

January 26, 2026

Conditions

Keywords

squamous cell carcinoma of the anusself-samplingswab performance characteristics

Outcome Measures

Primary Outcomes (1)

  • Presence of Squamocolumnar Cells

    The presence of cells from the squamocolumnar junction will be assessed in each specimen (Yes/No). Proportions of specimens with squamocolumnar cells will be compared for the 5 cm and 3 cm swabs.

    Day 1

Secondary Outcomes (4)

  • Cytologic Adequacy

    Day 1

  • User Acceptability of New NF Swab Compared to Swab From Prior Research

    Day 1

  • User Pain of New NF Swab Compared to Swab From Prior Research

    Day 1

  • User Pain by Swab Length

    Day 1

Study Arms (2)

Intervention arm

EXPERIMENTAL

Individuals in the intervention arm will self-sample with a swab and insert the swab 5 cm into the anus.

Other: Inserting a swab 5 cm into the anus during self-sampling

Control arm

ACTIVE COMPARATOR

Individuals in the control arm will self-sample with a swab and insert the swab 3 cm into the anus.

Other: Self-sampling with a swab by inserting the swab 3 cm into the anus.

Interventions

The swab will be inserted into the anus by the lay individual. The individual will insert the swab to a depth of 5 cm to collect anal exfoliated cells.

Intervention arm

The swab will be inserted into the anus by the lay individual. The individual will insert the swab to a depth of 3 cm to collect anal exfoliated cells.

Control arm

Eligibility Criteria

Age35 Years+
Sexmale
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Aged ≥ 35 years.
  • Must be either:
  • A sexual minority man, or
  • A woman who has a different identity than the sex she was born with.
  • Resides in the Milwaukee metropolitan area.
  • Speak and understand English.
  • Ability to understand a written informed consent document, and the willingness to sign it.

You may not qualify if:

  • \. Presence of any contraindicating severe anal disease or condition, e.g., anal stenosis.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Curative Building

Milwaukee, Wisconsin, 53226, United States

Location

MeSH Terms

Conditions

Anus Neoplasms

Condition Hierarchy (Ancestors)

Rectal NeoplasmsColorectal NeoplasmsIntestinal NeoplasmsGastrointestinal NeoplasmsDigestive System NeoplasmsNeoplasms by SiteNeoplasmsDigestive System DiseasesGastrointestinal DiseasesIntestinal DiseasesAnus DiseasesRectal Diseases

Results Point of Contact

Title
Alan Nyitray, PhD
Organization
Medical College of Wisconsin

Study Officials

  • Alan Nyitray, PhD

    Medical College of Wisconsin

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
No
Restrictive Agreement
No

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
SINGLE
Who Masked
OUTCOMES ASSESSOR
Purpose
SCREENING
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Associate Professor

Study Record Dates

First Submitted

July 10, 2025

First Posted

July 25, 2025

Study Start

July 31, 2025

Primary Completion

September 12, 2025

Study Completion

September 12, 2025

Last Updated

January 27, 2026

Results First Posted

November 18, 2025

Record last verified: 2025-12

Data Sharing

IPD Sharing
Will share

This Self-sampling to Optimize anal Lesion Outcomes (SOLO) pilot study will produce quantitative data generated from surveys with 60 participants. We will also collect data on intervention performance, biospecimen collection, and laboratory assays. Data from the 60 persons will be collected at 1 time point, the cytology appointment. To protect research participant identities, only de-identified individual quantitative data will be made available for sharing. The final datasets will include quantitative data as delineated in 1.A. We will share de-identified individual participant-level data. We will use typical strategies such as "safe harbor" methods that remove all protected health information (e.g., name, address, and contact information) per MCW policy AD.HP.090. Informed consent forms will reflect those plans. In circumstances where small sample sizes may allow identification of research participants, only summarized data will be made available for sharing.

Shared Documents
STUDY PROTOCOL, ICF
Time Frame
All scientific data generated from this project will be made available as soon as possible, and no later than the time of publication. The duration of preservation and sharing of the data will be a minimum of 10 years.
Access Criteria
The openICPSR provides metadata, persistent identifiers using ICPSR ID numbers and DOI, and long-term access. The repository is supported by the Inter-university Consortium for Political and Social Research (ICPSR) and datasets are available under a Creative Commons Attribution 4.0 International (CC BY 4.0) License. The dataset will be discoverable online by anyone through a standard web search of the study-level metadata as well as the persistent pointer from the ICPSR ID and DOI to the dataset.

Locations