NCT07080333

Brief Summary

In France, bladder cancer (BC) is the fourth leading cause of cancer (14,000 new cases per year). It affects older men (\>60 years old) and smoking is the main identified risk factor (RF). The persistence of a high sex ratio despite the increase in smoking among women has led to research into other environmental RFs for BC, which may include the microbiota. Recently, the concept of urinary microbiota in asymptomatic patients has been accepted. Studies on the urinary microbiota have shown dysbiosis associated with certain urogenital pathologies. Thus, similar to certain dysbiosis of the colonic mucosa microbiota associated with CRC, it is possible that certain bacteria or viruses in the bladder tissue microbiota may be involved in bladder carcinogenesis. An epidemiological study conducted by our laboratory showed a prevalence of BC of 14% (7/50) in patients carrying the bacterium Actinotignum schaalii in their urine. While studies have analysed the urinary microbiota of patients with BC, those comparing the microbiota of cancerous bladder tissue with that of healthy tissue in patients with BC remain few in number, involve a limited number of patients, use uncontrolled sample collection, and have all used 16S rDNA-targeted metagenomics methods to study the composition of the microbiota. The authors show a difference in biodiversity between the microbiota of cancerous bladder tissue and that of healthy tissue. The team hopes to confirm these preliminary results with a multicentre study using a more comprehensive genomic method, global metagenomics. The microbiota of cancerous bladder tissue will be compared with that of healthy bladder tissue in the same patient to highlight any bacterial or viral dysbiosis associated with the cancerous area.

Trial Health

63
Monitor

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
95

participants targeted

Target at P50-P75 for not_applicable

Timeline
31mo left

Started Oct 2025

Typical duration for not_applicable

Geographic Reach
1 country

8 active sites

Status
not yet recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress19%
Oct 2025Oct 2028

First Submitted

Initial submission to the registry

July 15, 2025

Completed
8 days until next milestone

First Posted

Study publicly available on registry

July 23, 2025

Completed
2 months until next milestone

Study Start

First participant enrolled

October 1, 2025

Completed
2.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 30, 2028

Expected
6 months until next milestone

Study Completion

Last participant's last visit for all outcomes

October 30, 2028

Last Updated

July 23, 2025

Status Verified

July 1, 2025

Enrollment Period

2.6 years

First QC Date

July 15, 2025

Last Update Submit

July 15, 2025

Conditions

Keywords

bladder; cancer; microbiota

Outcome Measures

Primary Outcomes (1)

  • The biodiversity of the bacterial bladder microbiota by global metagenomics

    description of biodiversity of bacterial bladder microbiota

    36 months

Secondary Outcomes (1)

  • Global biodiversity of viral microbiota by metagenomics

    36 months

Study Arms (1)

patient with suspected bladder cancer

EXPERIMENTAL
Biological: biopsy

Interventions

biopsyBIOLOGICAL

After verifying the patient's identity, obtaining their informed consent and confirming that there are no immediate contraindications to performing biopsies (haemorrhagic and infectious), the patient will be prepared for RTUV, which will be performed by cystoscopy under anaesthesia in the operating theatre.

patient with suspected bladder cancer

Eligibility Criteria

Age18 Years+
Sexmale
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Age \> 18 years old
  • Male
  • First RTUV for suspected CV in the presence of macroscopic haematuria or other clinical signs and/or urinary cytology and/or cystoscopy and/or imaging tests suggestive of CV
  • Signed informed consent form
  • Affiliation with a social security scheme

You may not qualify if:

  • Current urinary tract infection (urine culture recommended by the AFU prior to RTUV)
  • History of RTUV
  • History of pelvic radiotherapy
  • Interstitial cystitis
  • Patients with an indwelling urinary catheter
  • Vulnerable patients: minors, patients under guardianship or curatorship
  • Patients with benign tumours after receipt of the pathological report (inverted papilloma, non-tumour inflammatory lesion)

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (8)

Cannes Hospital

Cannes, 06141, France

Location

Marseille university Hospital

Marseille, 13000, France

Location

Menton Hospital

Menton, 06507, France

Location

Nice University Hospital

Nice, 06000, France

Location

Nimes University hospital

Nîmes, 30029, France

Location

Paris University Hospital

Paris, 75018, France

Location

Rennes University Hospital

Rennes, 35033, France

Location

Tours University Hospital

Tours, 37044, France

Location

MeSH Terms

Conditions

Neoplasms

Interventions

Biopsy

Intervention Hierarchy (Ancestors)

CytodiagnosisCytological TechniquesClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisSpecimen HandlingDiagnostic Techniques, SurgicalSurgical Procedures, OperativeInvestigative Techniques

Central Study Contacts

Romain LOTTE, MCUPH

CONTACT

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NA
Masking
NONE
Purpose
OTHER
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

July 15, 2025

First Posted

July 23, 2025

Study Start

October 1, 2025

Primary Completion (Estimated)

April 30, 2028

Study Completion (Estimated)

October 30, 2028

Last Updated

July 23, 2025

Record last verified: 2025-07

Data Sharing

IPD Sharing
Will not share

Locations