Investigation of the Effect of Horizontal Platelet Rich Fibrin Placed in Tooth Extraction Sockets on Bone Formation

NCT07011303 | Enrolling By Invitation

• 2 other identifiers: 23-KAEK-3202024/25
• Study Type: interventional
• Target: 30
• Locations: 1 country
• Sites: 1

Brief Summary

Dental implants, the treatment of complete and partial edentulous cases involving the restoration of lost teeth, have become a registered and widely used treatment option. Implants: It is an ideal option for the good oral health of the person who has lost his teeth due to periodontal disease, caries, trauma, or any other reason. When teeth are missing, melting occurs in the jawbone. Following tooth extraction, the loss of the implant and the amount of melting are significantly reduced. In implant treatments, which have become widely used today, successful results depend on the ideal volume and quality of hard and soft tissues. The amount of bone present in the edentulous area is important for the indication of the implant. The aim of this study is to evaluate the histological and immunohistochemical contribution of the H-PRF (horizontal-platelet rich fibrin) material placed in the extraction sockets to the quality of the newly formed bone tissue in order to make successful implants after tooth extractions for various reasons in humans. The research hypothesis is that H-PRF will contribute positively to the healing of tooth extraction sockets.

Conditions

Tooth Loss; Dental Implant Failed; Alveolar Bone Loss

Keywords

H-PRF, L-PRF, tooth extraction

Outcome Measures

Primary Outcomes (3)

• Maximum mouth opening measurements

  Preoperative and postoperative maximum mouth opening will be measured between the lower and upper middle incisal teeth.

  Preoperative, postoperative 2nd day and 7th day

• Pain level after extraction postoperative pain after tooth extraction

  Visual Analog Scale (VAS) will be used to assess pain.

  After 6th hour and 7th day (6th hour, 2nd day, 3rd day, 4th day, 5th day, 6th day, 7th day)

• Swelling

  Preoperative and postoperative edema will be measured according to the reference points.

  Postoperative 2nd and 7th days

Study Arms (3)

CONTROL GROUP

NO INTERVENTION

Control Group (n=10): Patient group in which no material was placed in the extraction socket after tooth extraction.

L-PRF (leukocyte-platelet rich fibrin)

ACTIVE COMPARATOR

L-PRF (Comparison Arm): The patient group in which L-PRF will be placed in the extraction socket after tooth extraction.

Procedure: L-PRF

H-PRF (horizontal-platelet rich fibrin)

EXPERIMENTAL

H-PRF (Research Branch): The patient group in which H-PRF will be placed in the extraction socket after tooth extraction.

Procedure: H-PRF

Interventions

H-PRF PROCEDURE

Blood will be taken from the patient before the surgical procedure is started. The blood sample will be placed in the H-TZF device for centrifugation and the sample will be horizontally centrifuged (2200 RPMx8 min, 700g). After the blood is processed for centrifugation, the surgery will begin. Surgery will be performed under local anesthesia with articaine 2 ml 4% and epinephrine 1:100,000. All patients will be operated by the same surgeon. After local anesthesia is achieved, tooth extraction will be started. After tooth extraction, the surgical field will be washed with sterile 0.9% saline, and H-PRF material will be placed in the area and primarily sutured with a 3.0 silk suture. All patients will be given postoperative instructions by the surgeon. Patients will be prescribed mouthwash (0.12% chlorhexidine mouthwash twice daily for 7 days).

H-PRF (horizontal-platelet rich fibrin)

L-PRF PROCEDURE

Blood will be taken from the patient before the surgical procedure is started. The blood sample will be placed in the L-PRF device for centrifugation and the sample will be centrifuged. (2700M x 12 min, 700g) After the blood is processed for centrifugation, the surgery will begin. Surgery will be performed under local anaesthesia with articaine 2 ml 4% and epinephrine 1:100,000. All patients will be operated by the same surgeon. After local anaesthesia is achieved, tooth extraction will be started. After tooth extraction, the surgical field will be washed with sterile 0.9% saline, and L-PRF material will be placed in the area and primarily sutured with a 3.0 silk suture. All patients will be given postoperative instructions by the surgeon. Patients will be prescribed mouthwash (0.12% chlorhexidine mouthwash twice daily for 7 days).

L-PRF (leukocyte-platelet rich fibrin)

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Volunteer ASA I individuals between the ages of 18-40
• Individuals with indication for tooth extraction
• Patients who want to have implants after tooth extraction

You may not qualify if:

• Those who are pregnant or lactating
• Those receiving anticoagulant or antiplatelet drug therapy
• Those who received radiotherapy to the head and face area

Sponsors & Collaborators

• Tokat Gaziosmanpasa University: lead

Study Sites (1)

Tokat Gaziosmanpasa University, Faculty of Dentistry

Tokat Province, 60100, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Tooth Loss; Alveolar Bone Loss

Condition Hierarchy (Ancestors)

Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases; Tooth Diseases; Bone Resorption; Bone Diseases; Musculoskeletal Diseases; Periodontal Atrophy

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: PREVENTION
• Intervention Model: FACTORIAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Dr. faculty member

Study Record Dates

• First Submitted: December 12, 2024
• First Posted: June 8, 2025
• Study Start: June 1, 2024
• Primary Completion: November 1, 2024
• Study Completion: April 1, 2026
• Last Updated: January 26, 2026

Record last verified: 2026-01

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)