Amino Acid Metabolism Before and After Bariatric Surgery

NCT07008352 | Completed

• 1 other identifier: Gerger
• Study Type: observational
• Target: 100
• Locations: 1 country
• Sites: 1

Brief Summary

This observational study aims to investigate the changes in serum amino acid metabolism before and after bariatric surgery in obese individuals. Blood samples will be collected from 50 patients at baseline (preoperative), and at 1 and 3 months postoperatively. An additional 50 healthy individuals will serve as the control group. Amino acid profiling will be conducted using LC-MS/MS. The study seeks to determine how bariatric surgery affects metabolic pathways associated with amino acid regulation.

Conditions

Obesity, Morbid

Outcome Measures

Primary Outcomes (1)

• Change in serum free amino acid levels after bariatric surgery

  Amino acid concentrations will be measured in serum samples collected from bariatric surgery patients at baseline, 1 month, and 3 months postoperatively using LC-MS/MS. The outcome will assess time-dependent changes in the amino acid profile within the same individuals.

  Preoperative (baseline), 1 month postoperative, 3 months postoperative

Secondary Outcomes (1)

• Correlation between serum amino acid levels and body mass index (BMI)

  Preoperative (baseline), 1 month postoperative, 3 months postoperative

Study Arms (2)

Group 1: Bariatric surgery patients (n=50)

Participants in this group are adults (≥18 years old) with obesity who are scheduled to undergo bariatric surgery at Harran University Hospital. Blood samples will be collected at three time points: before surgery (baseline), and at 1 and 3 months postoperatively. Serum will be separated and stored at -80°C for subsequent amino acid profiling using LC-MS/MS.

Procedure: Blood sample collection for amino acid profiling

Group 2: Healthy control participants (n=50)

Participants in this group are healthy adults (≥18 years old) with no known history of obesity or chronic metabolic disease. A single blood sample will be collected at baseline. Serum samples will be analyzed using LC-MS/MS to determine amino acid profiles, which will be compared to those of the bariatric surgery group.

Procedure: Blood sample collection for amino acid profiling

Interventions

Blood sample collection for amino acid profiling PROCEDURE

Blood samples (5 mL) will be collected from participants in both groups. In the bariatric surgery group, samples will be collected at three time points: preoperatively, and at 1 and 3 months postoperatively. For the control group, a single sample will be obtained. Serum will be separated, stored at -80°C, and analyzed for free amino acid profiles using LC-MS/MS.

Group 1: Bariatric surgery patients (n=50); Group 2: Healthy control participants (n=50)

Eligibility Criteria

• Age: 18 Years - 65 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Participants will include 50 patients diagnosed with obesity and undergoing bariatric surgery, and 50 healthy adults with normal BMI values. All participants will be recruited from Harran University Hospital.

You may qualify if:

• Age ≥18 years
• Diagnosed with obesity and scheduled for bariatric surgery (study group)
• Healthy individuals with normal BMI (control group)

You may not qualify if:

• Age \<18 years
• History of metabolic or chronic systemic disease
• Pregnancy or lactation

Sponsors & Collaborators

• Harran University: lead

Study Sites (1)

Veli Fahri Pehlivan

Sanliurfa, 63100, Turkey (Türkiye)

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

Samples with DNA or without DNA will be retained

MeSH Terms

Conditions

Obesity, Morbid

Condition Hierarchy (Ancestors)

Obesity; Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor Dr

Study Record Dates

• First Submitted: May 29, 2025
• First Posted: June 6, 2025
• Study Start: June 11, 2025
• Primary Completion: October 2, 2025
• Study Completion: November 3, 2025
• Last Updated: November 26, 2025

Record last verified: 2025-11

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)