Cerebral Large Vessel Occlusion Stroke Multiomics Biosample Cohort

NCT06963489 | Recruiting DMC

• 1 other identifier: 202543
• Study Type: observational
• Target: 500
• Locations: 1 country
• Sites: 5

Brief Summary

This multicenter, ambispective cohort study establishes a comprehensive multiomics biobank from five stroke centers, encompassing thrombi, intracranial blood, peripheral arterial/venous blood, and clinical-laboratory-imaging-follow-up data from patients with acute ischemic stroke with large vessel occlusion (AIS-LVO).

Conditions

Large Vessel Occlusion; Acute Ischemic Stroke

Keywords

multiomics; acute ischemic stroke with large vessel occlusion

Outcome Measures

Primary Outcomes (1)

• Number of patients with futile recanalization

  mRS score ≥3 at 3 months

  From enrollment to 3 month follow-up

Secondary Outcomes (3)

• Number of patients with specific stroke etiologies

  Baseline

• Number of patients with hemorrhagic transformation

  7 days within stroke onset

• Number of patients with infarct expansion

  Baseline

Interventions

Not applicable- observational study OTHER

Intervention is not applicable

Eligibility Criteria

• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

We include patients with acute large vessel occlusion ischemic stroke from at least 5 stroke centers in China.

You may qualify if:

• Age over 18 years.
• Patients with intracranial and extracranial large vessel occlusion ischemic stroke confirmed by cerebrovascular angiography.
• Availability of complete clinical and follow-up information required for the study.
• Availability of blood and/or thrombus biological samples.
• Voluntary written informed consent signed by the patient or their family (1) For retrospectively enrolled patients, a "broad informed consent" was signed at the time of sample collection. Upon enrollment into the cohort, a follow-up phone call was made to confirm the informed consent.
• (2) For prospectively enrolled patients, informed consent for this study was signed at the time of enrollment by the patient or their family.

You may not qualify if:

• Patients for whom biological samples cannot be obtained;
• Patients or their family members who refuse to sign the informed consent form.

Sponsors & Collaborators

• First Affiliated Hospital of Wannan Medical College: lead
• Beijing Tiantan Hospital: collaborator
• The First Affiliated Hospital of Zhengzhou University: collaborator
• Baotou Central Hospital: collaborator
• Affiliated Hospital of Shandong University of Traditional Chinese Medicine: collaborator

Study Sites (5)

The First Affiliated Hospital of Shihezi University

Wuhu, Anhui, China

NOT YET RECRUITING

Baotou Central Hospital

Baotou, Inner Mongolia, China

RECRUITING

Affiliated Hospital of Shandong University of Traditional Chinese Medicine Hospital

Jinan, Shandong, China

RECRUITING

The First Affiliated Hospital of zhengzhou University

Henan, Zhengzhou, China

RECRUITING

Capital medical university of Beijing Tiantan Hospital

Beijing, 100070, China

RECRUITING

Related Publications (11)

• Xu X, Song Y, Cao W, Bai X, Wang X, Gao P, Chen J, Chen Y, Yang B, Wang Y, Chen F, Ma Q, Yu B, Jiao L. Alterations of Hemostatic Molecular Markers During Acute Large Vessel Occlusion Stroke. J Am Heart Assoc. 2024 Feb 6;13(3):e032651. doi: 10.1161/JAHA.123.032651. Epub 2024 Jan 31.

  PMID: 38293908 BACKGROUND

• Kollikowski AM, Schuhmann MK, Nieswandt B, Mullges W, Stoll G, Pham M. Local Leukocyte Invasion during Hyperacute Human Ischemic Stroke. Ann Neurol. 2020 Mar;87(3):466-479. doi: 10.1002/ana.25665. Epub 2020 Jan 16.

  PMID: 31899551 BACKGROUND

• Strinitz M, Pham M, Marz AG, Feick J, Weidner F, Vogt ML, Essig F, Neugebauer H, Stoll G, Schuhmann MK, Kollikowski AM. Immune Cells Invade the Collateral Circulation during Human Stroke: Prospective Replication and Extension. Int J Mol Sci. 2021 Aug 25;22(17):9161. doi: 10.3390/ijms22179161.

  PMID: 34502070 BACKGROUND

• Stoll G, Schuhmann MK, Nieswandt B, Kollikowski AM, Pham M. An intravascular perspective on hyper-acute neutrophil, T-cell and platelet responses: Similarities between human and experimental stroke. J Cereb Blood Flow Metab. 2022 Sep;42(9):1561-1567. doi: 10.1177/0271678X221105764. Epub 2022 Jun 8.

  PMID: 35676801 BACKGROUND

• Zimmermann L, Pham M, Marz AG, Kollikowski AM, Stoll G, Schuhmann MK. Defining cerebral leukocyte populations in local ischemic blood samples from patients with hyperacute stroke. J Cereb Blood Flow Metab. 2022 May;42(5):901-904. doi: 10.1177/0271678X221078617. Epub 2022 Feb 2.

  PMID: 35107055 BACKGROUND

• Dargazanli C, Blaquiere M, Moynier M, de Bock F, Labreuche J, Ter Schiphorst A, Derraz I, Radu RA, Gascou G, Lefevre PH, Rapido F, Fendeleur J, Arquizan C, Bourcier R, Marin P, Machi P, Cagnazzo F, Hirtz C, Costalat V, Marchi N. Inflammation biomarkers in the intracranial blood are associated with outcome in patients with ischemic stroke. J Neurointerv Surg. 2025 Jan 17;17(2):159-166. doi: 10.1136/jnis-2023-021365.

  PMID: 38514190 BACKGROUND

• Costamagna G, Bonato S, Corti S, Meneri M. Advancing Stroke Research on Cerebral Thrombi with Omic Technologies. Int J Mol Sci. 2023 Feb 8;24(4):3419. doi: 10.3390/ijms24043419.

  PMID: 36834829 BACKGROUND

• Staessens S, Fitzgerald S, Andersson T, Clarencon F, Denorme F, Gounis MJ, Hacke W, Liebeskind DS, Szikora I, van Es A, Brinjikji W, Doyle KM, De Meyer SF. Histological stroke clot analysis after thrombectomy: Technical aspects and recommendations. Int J Stroke. 2020 Jul;15(5):467-476. doi: 10.1177/1747493019884527. Epub 2019 Nov 3.

  PMID: 31679478 BACKGROUND

• Staessens S, Francois O, Brinjikji W, Doyle KM, Vanacker P, Andersson T, De Meyer SF. Studying Stroke Thrombus Composition After Thrombectomy: What Can We Learn? Stroke. 2021 Nov;52(11):3718-3727. doi: 10.1161/STROKEAHA.121.034289. Epub 2021 Sep 14.

  PMID: 34517770 BACKGROUND

• Patil S, Darcourt J, Messina P, Bozsak F, Cognard C, Doyle K. Characterising acute ischaemic stroke thrombi: insights from histology, imaging and emerging impedance-based technologies. Stroke Vasc Neurol. 2022 Aug;7(4):353-363. doi: 10.1136/svn-2021-001038. Epub 2022 Mar 3.

  PMID: 35241632 BACKGROUND

• Widimsky P, Snyder K, Sulzenko J, Hopkins LN, Stetkarova I. Acute ischaemic stroke: recent advances in reperfusion treatment. Eur Heart J. 2023 Apr 7;44(14):1205-1215. doi: 10.1093/eurheartj/ehac684.

  PMID: 36477996 BACKGROUND

Biospecimen

Retention: SAMPLES WITHOUT DNA

intracranial thrombi, intracranial occluded segment blood, peripheral arterial blood, peripheral venous blood,

MeSH Terms

Conditions

Ischemic Stroke

Condition Hierarchy (Ancestors)

Stroke; Cerebrovascular Disorders; Brain Diseases; Central Nervous System Diseases; Nervous System Diseases; Vascular Diseases; Cardiovascular Diseases

Central Study Contacts

Zhiyuan Feng, MD

CONTACT

86+15801290121; fzyhhz1996@gmail.com

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: OTHER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Deputy Director

Study Record Dates

• First Submitted: April 28, 2025
• First Posted: May 9, 2025
• Study Start: April 27, 2025
• Primary Completion (Estimated): February 29, 2028
• Study Completion (Estimated): February 29, 2028
• Last Updated: May 11, 2025

Record last verified: 2025-04

Locations

CN (5)