The Mechanism of Endogenous Nociceptin/Orphanin FQ(N/OFQ) and β1-adrenoceptor Autoantibody in Promoting Ischemic Arrhythmia in Rats

NCT06947551 | Not Yet Recruiting DMC

• 1 other identifier: HY20240410
• Study Type: observational
• Target: 60
• Locations: 0 countries
• Sites: N/A

Brief Summary

In the early stage of ischemic arrhythmia, endogenous N/OFQ regulates the expression of β 1-AA, mediates the internalization of β 1-AR by PKC/ERK1/2, and thus affects the occurrence of arrhythmia. Further clarify the regulatory mechanism of N/OFQ on β 1-AR internalization and arrhythmia, providing experimental basis for drug development and target exploration of clinical ischemic arrhythmia; To observe the relationship between endogenous N/OFQ and β 1-AA in serum of patients with coronary heart disease and diabetes.

Conditions

Acute Myocardial Ischemia

Outcome Measures

Primary Outcomes (1)

• Measurement of Orphanin and β 1-AA in Serum of Patients with Coronary Heart Disease or diabetes of Different Course

  To measure the content of orphanin and β 1-AA in the serum of patients with coronary heart disease or diabetes of different disease courses, and to explore the relationship between orphanin and β 1-AA in the serum of patients with different disease courses, so as to provide support for the prediction of clinical coronary heart disease or diabetes.

  The patient's first blood draw upon admission

Study Arms (1)

Patients with coronary heart disease or diabetes under different course of disease

Select patients with coronary heart disease and diabetes who meet the standard, take 8ml of blood from the elbow vein, 4ml of blood for centrifugation, take serum, and use chromatography column to separate and extract β 1-AA; 4ml is used to detect endogenous N/OFQ and β 1-AA levels using an ELISA kit.

Eligibility Criteria

• Age: 18 Years - 85 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

Patients with coronary heart disease or diabetes under different course of disease

Sponsors & Collaborators

• Second Hospital of Shanxi Medical University: lead

Study Officials

• yi Han

  Second Hospital of Shanxi Medical University

  STUDY DIRECTOR

Central Study Contacts

xinyu shang

CONTACT

18451655670; 13513520761@163.com

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Study Record Dates

• First Submitted: April 20, 2025
• First Posted: April 27, 2025
• Study Start: May 1, 2025
• Primary Completion: May 1, 2026
• Study Completion (Estimated): June 1, 2026
• Last Updated: April 27, 2025

Record last verified: 2025-04

Data Sharing

• IPD Sharing: Will not share