Eccentric Cycling Exercise With Assistive Devices on Mitochondrial Metabolism in T Lymphocyte

NCT06905964 | Completed

• 1 other identifier: 202202314A3
• Study Type: interventional
• Target: 30
• Locations: 1 country
• Sites: 1

Brief Summary

Eccentric cycling exercise can be a promising modality fwith lower respiratory demands and higher power output. Assistive devices like neuromuscular electrical stimulation (NMES) and compressive garment (CG) accelerates muscle pumping and relieve the eccentric exercise-induced muscle soreness. Phenotypes and mitochondrial metabolism are crucial to regulate acquired immnunity of T lymphocytes. The study aimed to investigate the effects of eccentric cycling exercise training combined with NMES and CG on mitochondrial phenotypes and bioenergetics in T lymphocytes in sedentary males. The hypothesis were an acute/intense exercise decreased the mitochondrial respiratory capacities of OXPHOS, ETS and bioenergetics, while eccentric cycling training could elevate the diminished mitochondrial functions. Methods: Twelve sedentary healthy males were randomly assigned to either eccentric cycling with assistive devices (ECA, n=10) or eccentric cycling only (ECC, n=10). All participants performed ECA or ECC at an intensity of 60% to 120% maximal workload progressively on a costumed bicycle ergometer for 40 min/day, 5 days/week for 5 weeks. ECA performed NMES for 20min/session after cycling, while ECC was seated for resting 20 min. Systemic aerobic fitness, phenotypes of T lymphocytes, mitochondrial OXPHOS, ETS respiratory capacities, and bioenergetics in T lymphocytes were analyzed.

Conditions

There Are no Conditions Under Study. Healthy Volunteers

Keywords

Eccentric cycling exercise; Neuromuscular electrical stimulation; Compressive garment; Lymphocyte; Mitochondria

Outcome Measures

Primary Outcomes (2)

• Mitochondrial bioenergetics of oxygen consumption rate in T lymphocyte

  The bioenergetic health index (BHI) of mitochindria in T lymphocyte was measured. BHI is a parameter for detecting the health status of mitochondrial and efficiency of energy metabolism.

  At baseline and 2 days after completing the 5-week intervention

• Systemic aerobic fitness (VO2)

  VO2max was determined during the exercise testing. VO2max is the maximum of oxygen deliver and utilization.

  At baseline and 2 days after completing the 5-week intervention

Study Arms (3)

Eccentric cycling exercise (ECC)

EXPERIMENTAL

High intensity cycling training from 80% to 120% Wmax in eccentric type

Behavioral: Eccentric cycling exercise (ECC)

Eccentric cycling exercise with assistive devices (ECA)

EXPERIMENTAL

High intensity cycling training from 80% to 120% Wmax in eccentric type (intensity matched the ECC)

Behavioral: Eccentric cycling exercise with assistive devices (ECA)

Control group

NO INTERVENTION

Perform no exercise training but maintain only regular daily lives only in the study.

Interventions

Eccentric cycling exercise (ECC) BEHAVIORAL

Performed exercise training 5 days a week for 5 weeks on an eccentric ergometer. Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase. After each training session, rest in sitting position for 20 min. Exercise intensity: Firstly, set at 80% Wmax, and progressively increased 5% per week to 120 % Wmax in the last week.

Eccentric cycling exercise (ECC)

Eccentric cycling exercise with assistive devices (ECA) BEHAVIORAL

Performed exercise training 5 days a week for 5 weeks on an eccentric ergometer. Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase. After each training session, receive NMES in sitting position for 20 min. Intensity: Firstly, set at 80% Wmax, and progressively increased 5% per week to 120 % Wmax in the last week.

Eccentric cycling exercise with assistive devices (ECA)

Eligibility Criteria

• Age: 20 Years - 30 Years
• Gender Eligibility Details: Due to the blood experiments, we recruited male participants only to avoid interference of menstrual cycle.
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Sedentary lifestyle (exercise\<20min/session; \<1session/week for a year or longer).
• Nonsmokers, nonusers of medications/vitamins.
• No any cardiopulmonary, neuromuscular, and hematological risks.

You may not qualify if:

• Have regular exercise habits (i.e., exercise frequency once per week, duration \>20 min).
• Have retabolic or musculoskeletal disease.

Sponsors & Collaborators

• Chang Gung Memorial Hospital: lead

Study Sites (1)

Chang Gung University

Guishan, Taoyuan, 333, Taiwan

Location

Related Publications (3)

• Huang YC, Cheng ML, Tang HY, Huang CY, Chen KM, Wang JS. Eccentric Cycling Training Improves Erythrocyte Antioxidant and Oxygen Releasing Capacity Associated with Enhanced Anaerobic Glycolysis and Intracellular Acidosis. Antioxidants (Basel). 2021 Feb 13;10(2):285. doi: 10.3390/antiox10020285.

  PMID: 33668606 BACKGROUND

• Peng SC, Lin YT, Hsu CC, Fu TC, Wang JS. Effects of concentric and eccentric exercise regimens on bioenergetic efficiency of lymphocytes in sedentary males. Eur J Sport Sci. 2024 Dec;24(12):1830-1843. doi: 10.1002/ejsc.12221. Epub 2024 Nov 9.

  PMID: 39520384 BACKGROUND

• Huang YC, Hsu CC, Fu TC, Wang JS. Interval aerobic/resistance exercise training depresses adrenergic-induced apoptosis of lymphocytes in sedentary males. Eur J Appl Physiol. 2024 Mar;124(3):837-848. doi: 10.1007/s00421-023-05311-3. Epub 2023 Sep 15.

  PMID: 37712975 BACKGROUND

MeSH Terms

Interventions

Self-Help Devices

Intervention Hierarchy (Ancestors)

Equipment and Supplies

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: January 8, 2025
• First Posted: April 2, 2025
• Study Start: February 23, 2023
• Primary Completion: February 23, 2024
• Study Completion: December 31, 2024
• Last Updated: April 2, 2025

Record last verified: 2025-01

Data Sharing

• IPD Sharing: Will not share

Locations

TW (1)