The Impact of Dietary Intervention on Oxidative/antioxidant Markers and Gut Microbiota in Athletes

NCT06855979 | Completed

• 3 other identifiers: APK.002.405.2024SKN/SP/601112/2024B.SUB.25.207
• Study Type: interventional
• Target: 50
• Locations: 1 country
• Sites: 1

Brief Summary

During intense physical exercise, there is an overproduction of reactive oxygen species, which leads to oxidative stress and reduced training and sports performance, as well as the development of chronic diseases. Eating foods with a high content of bioactive ingredients and high antioxidant potential can alleviate the negative effects caused by reactive oxygen species and improve the state of intestinal microflora. The aim of these interventional studies was to determine whether daily consumption of foods with high antioxidant potential, including fruit and nut bars, for a period of 1 month would reduce oxidative stress in athletes during competition and positively change the intestinal microflora.

Conditions

Non Communicable Diseases

Keywords

sport; cardiovascular disease; antioxidant stress

Outcome Measures

Primary Outcomes (7)

• Change in antioxidant potential after dietary intervention

  Before and after the dietary intervention, the antioxidant potential in the blood was determined using the ABTS method.

  4 weeks

• Change in gut microbiota after dietary intervention

  The gut microbiota in feces was determined before and after the dietary intervention in a hospital laboratory.

  4 weeks

• Assessment of dietary intervention

  The dietary intervention lasted 4 weeks. The subjects received one bar a day weighing 50 g, containing nuts and dried fruits with high antioxidant potential and high polyphenol content. After the dietary intervention, the participants completed a survey containing questions about compliance with the recommendations of the dietary intervention.

  4 weeks

• Change in blood glutathione peroxidase concentration after dietary intervention

  Before and after the dietary intervention, the concentration of glutathione peroxidase enzyme in blood was assessed using ready-made laboratory kits.

  4 weeks

• Change in superoxide dismutase concentration after dietary intervention

  Before and after the dietary intervention, the concentration of superoxide dismutase enzyme in blood was assessed using ready-made laboratory kits.

  4 weeks

• Change in catalase concentrations after dietary intervention

  Before and after the dietary intervention, the concentration of catalase enzyme in blood was assessed using ready-made laboratory kits.

  4 weeks

• Change in oxidative stress after dietary intervention

  Before and after the dietary intervention, the oxidative stress in blood was assessed using ready-made laboratory kits.

  4 weeks

Secondary Outcomes (3)

• Assessment of dietary nutrients before and after dietary intervention

  4 weeks

• Body weight assessment before and after dietary intervention

  4 weeks

• Measuring the height of study participants

  4 weeks

Study Arms (1)

Oxidative-antioxidant markers in blood and gut microbiota

EXPERIMENTAL

Before and after the 1-month dietary intervention, the following markers will be determined in the blood: antioxidant potential, antioxidant enzymes, oxidative stress. Moreover, the intestinal microbiota will be determined before and after the intervention.

Other: Food with high antioxidant potential

Interventions

Food with high antioxidant potential OTHER

The dietary intervention using fruit and nut bars with high antioxidant activity will last 1 month. Before and after the procedure, oxidative-antioxidant markers in the blood and intestinal microflora in the stool will be determined.

Oxidative-antioxidant markers in blood and gut microbiota

Eligibility Criteria

• Age: 20 Years - 50 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• healthy men and women with high physical activity (triathletes, cyclists, long-distance runners), not taking medications for chronic diseases, age 20-50 years, men and women.

You may not qualify if:

• occurence of chronic diseases (e.g. hypertension, diabetes, cancer, thyroid diseases, neurodegenerative diseases), taking medications for chronic diseases, age\<20 and \>50 years, low physical activity

Sponsors & Collaborators

• Medical University of Bialystok: lead

Study Sites (1)

Medical University of Bialystok

Bialystok, 15-089, Poland

Location

MeSH Terms

Conditions

Noncommunicable Diseases; Cardiovascular Diseases

Interventions

Food

Condition Hierarchy (Ancestors)

Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Diet, Food, and Nutrition; Physiological Phenomena; Food and Beverages

Study Officials

• Malgorzata E Zujko, Prof.

  Medical University of Bialystok, Jana Kilinskiego 1, 15-089 Bialystok

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: PREVENTION
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: February 20, 2025
• First Posted: March 4, 2025
• Study Start: January 15, 2025
• Primary Completion: February 15, 2025
• Study Completion: February 15, 2025
• Last Updated: March 4, 2025

Record last verified: 2025-02

Data Sharing

• IPD Sharing: Will share
• Shared Documents: CSR
• Time Frame: The clinical trial report will be published in scientific journals after the study is completed.
• Access Criteria: Additional information can be obtained by writing to the principal investigator's address: malgorzata.zujko@umb.edu.pl.

Locations

PL (1)