Impact of Dietary Nitrate Supplementation on VO2max During Ramadan Fasting in Healthy Adults

NCT06767176 | Completed

• 1 other identifier: 25-01-006
• Study Type: interventional
• Target: 21
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this clinical trial is to determine if beetroot juice supplementation changes exercise performance following one week of Ramadan fasting in young, healthy adults. The main question it aims to answer is: Does daily beetroot juice supplementation change VO2 max in fasting individuals during the first week of Ramadan? Participants will: Visit the lab before the start of Ramadan and after one week of fasting If assigned to intervention group, consume daily shots of beetroot juice (70ml) during the first week of Ramadan fasting Perform maximal exercise testing on a cycle ergometer

Conditions

Ramadan Fasting

Keywords

Ramadan; Fasting; VO2max; Dietary Nitrate

Outcome Measures

Primary Outcomes (1)

• VO2 max

  Maximal Oxygen Uptakes will be assessed to analyze aerobic capacity and fitness under fasting conditions.

  One--week differences between before and after 1 week of fasting, nitrate supplementation if in intervention group

Secondary Outcomes (1)

• Nitrate Concentration levels

  Comparison between blood before and after nitrate supplementation: one week

Study Arms (2)

No Intervention

NO INTERVENTION

Normal Fasting, no intervention. During this arm, participants will be asked to complete an exercise test before and after 1 week of Ramadan fasting.

Nitrate Supplementation

EXPERIMENTAL

Participants will be asked to consume daily nitrate supplementation (70ml) in the form of beetroot juice for 7 days. Participants will then complete a maximal exercise test as they fast.

Dietary Supplement: Beetroot Juice (Beet-It Stamina Shot) & Supervised Exercise Training

Interventions

Beetroot Juice (Beet-It Stamina Shot) & Supervised Exercise Training DIETARY_SUPPLEMENT

7 doses, taken daily, 70 ml shots, consumed in the morning before the start of fasting

Nitrate Supplementation

Eligibility Criteria

• Age: 18 Years - 50 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64)

You may qualify if:

• Free of known cardiovascular or metabolic diseases or sleep disorders
• No history of smoking (within the past 3 months)
• Able to engage in physical activity assessed through the physical activity readiness questionnaire (PAR-Q+)
• No prescription of chronic medications other than oral contraceptives
• Able to abide by fasting protocols for all visits
• Individuals who are not allergic to ultrasound gel or beetroot juice
• Individuals who are not pregnant

You may not qualify if:

• Individuals diagnosed with cardiovascular or metabolic disease or sleep disorders
• Has a history of smoking (within the past 3 months)
• Not ready to engage in physical activity as assessed by the PAR-Q+
• Individuals prescribed chronic medications other than oral contraceptives
• Unable to abide by fasting protocols for any testing visit
• Allergic to ultrasound gel or beetroot juice
• Pregnancy

Sponsors & Collaborators

• University of Guelph: lead

Study Sites (1)

University of Guelph

Guelph, Canada

Location

Related Links

• Intervention Wesbite.

MeSH Terms

Conditions

Fasting

Condition Hierarchy (Ancestors)

Feeding Behavior; Behavior

Study Officials

• Phillip Millar, PhD

  University of Guelph

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: January 6, 2025
• First Posted: January 9, 2025
• Study Start: January 6, 2025
• Primary Completion: March 31, 2025
• Study Completion: March 31, 2025
• Last Updated: May 1, 2026

Record last verified: 2026-04

Data Sharing

• IPD Sharing: Will share
• Time Frame: Beginning after publication with no end date
• Access Criteria: Phillip Millar, University of Guelph

Locations

CA (1)