Integrative Analysis of Exosome-Mediated Single-Cell Transcriptomics and Proteomics in Gastric Cardia Cancer

NCT06702891 | Recruiting

• 1 other identifier: 2024SDU-QILU-2
• Study Type: observational
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

This is an observational study with a case control design. This study included patients with gastric cancer confirmed by pathological diagnosis of gastric tissue, and matched the control population according to age, gender, etc. In this study, researchers collected clinical information and multiple biological samples such as serum and tissue tissue from the study subjects. The researchers combined exosome sequencing, proteomics and single-cell RNA sequencing to explore the heterogeneity of cardia cancer, identify specific biomarkers and potential therapeutic targets for cardia cancer, and provide a basis for the development of new diagnostic tools and therapeutic strategies.

Conditions

Gastric Cardia Cancer

Keywords

Gastric Cardia Cancer; Single-Cell RNA Sequencing; Exosomes; Proteomics

Outcome Measures

Primary Outcomes (1)

• Differential protein

  6 months

Secondary Outcomes (2)

• Key miRNA

  10 months

• Differential cell subsets

  12 months

Study Arms (2)

case group

The case group includes high-grade intraepithelial neoplasia of the stomach, early gastric cancer, and advanced gastric cancer.

Other: Detection of biological samples based on exosome sequencing, proteomics and single-cell RNA sequencing

control group

Pathological findings in the control group showed no malignant changes in the stomach, including normal gastric mucosa, superficial gastritis, non atrophic gastritis, and gastric polyps, etc.

Other: Detection of biological samples based on exosome sequencing, proteomics and single-cell RNA sequencing

Interventions

Detection of biological samples based on exosome sequencing, proteomics and single-cell RNA sequencing OTHER

The researchers combined exosome sequencing, proteomics and single-cell RNA sequencing to explore the heterogeneity of cardia cancer, identify specific biomarkers and potential therapeutic targets for cardia cancer.

case group; control group

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Case: The population in the case group signed an informed consent form, aged 18 years old or older. All subjects underwent endoscopic examination and were confirmed by pathological diagnosis as gastric cancer, including high-grade intraepithelial neoplasia, early gastric cancer, and advanced gastric cancer. Control: The age and gender of the study subjects in the control group were matched to the study population in the case group. The pathological results of the stomach of all subjects showed no malignant changes, including normal gastric mucosa, superficial gastritis, non atrophic gastritis, and gastric polyps, etc.

You may qualify if:

• Study population signing informed consent form
• Age: 18 years old or older
• The study population must underwent endoscopic examination and was confirmed by pathological diagnosis as gastric cancer: including high-level intraepithelial neoplasia, early gastric cancer, and advanced gastric cancer.

You may not qualify if:

• Those who have undergone gastrointestinal surgery within the past 1 year; Patients who have undergone neoadjuvant chemotherapy and have developed tumors in the residual stomach after previous partial gastrectomy.
• Those who have used proton pump inhibitors, antibiotics, probiotics, and prebiotics daily within the past month, and those who have recently received hormone therapy.
• People with infectious diseases and other digestive system diseases that interfere with the experimental results, such as inflammatory bowel disease, irritable bowel syndrome, etc.

Sponsors & Collaborators

• Qilu Hospital of Shandong University: lead

Study Sites (1)

Qilu Hospital of Shandong University

Jinan, Shandong, 250012, China

RECRUITING

MeSH Terms

Interventions

Single-Cell Gene Expression Analysis

Intervention Hierarchy (Ancestors)

Single-Cell Analysis; Cytological Techniques; Investigative Techniques; Gene Expression Profiling; Genetic Techniques

Central Study Contacts

Xiaoya Wang, Bachelor

CONTACT

(86)13821379210; xiaoyawang99@163.com

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Target Duration: 2 Years
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: November 18, 2024
• First Posted: November 25, 2024
• Study Start: December 17, 2024
• Primary Completion: October 1, 2026
• Study Completion (Estimated): October 1, 2027
• Last Updated: September 3, 2026

Record last verified: 2026-08

Locations

CN (1)