NCT06694363

Brief Summary

The study would like to compare patient samples at different time points using state-of the art-phenotyping tools. Collection of blood samples of APDS patients undergoing PI3K inhibitor treatment will be collected when feasible according to the standard of care planning (a blood test is supposed to be performed for these patients at M0-M3-M6-M12 then each 6 months for a total period of 2 years from the beginning of the PI3K inhibitor treatment). The whole blood will be processed in order to isolate the peripheral blood mononuclear cells (PBMC) and the plasma. Serum, RNA and DNA extraction will be performed on a separate sample.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
14

participants targeted

Target at below P25 for all trials

Timeline
44mo left

Started Jun 2025

Longer than P75 for all trials

Geographic Reach
1 country

7 active sites

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress19%
Jun 2025Dec 2029

First Submitted

Initial submission to the registry

November 15, 2024

Completed
4 days until next milestone

First Posted

Study publicly available on registry

November 19, 2024

Completed
7 months until next milestone

Study Start

First participant enrolled

June 26, 2025

Completed
4 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 27, 2029

Expected
6 months until next milestone

Study Completion

Last participant's last visit for all outcomes

December 27, 2029

Last Updated

March 23, 2026

Status Verified

March 1, 2026

Enrollment Period

4 years

First QC Date

November 15, 2024

Last Update Submit

March 19, 2026

Conditions

Keywords

Activated PI3K delta syndromesAPDSLeniolisib

Outcome Measures

Primary Outcomes (1)

  • Biomarker

    Identify new biomarker marker for increased PI3K signaling to monitor the disease severity by mass cytometry, single cell RNA-sequencing, single cell ATAC-sequencing and screening for auto-antibodies

    At 0 day, 3 months, 6 months, 12 months, 18 months and 24 months of treatment

Secondary Outcomes (3)

  • activation-induced cytidine deaminase (AID) off-target activity

    At 0 day, 3 months, 6 months, 12 months, 18 months and 24 months of treatment

  • Correlation between biological data with the clinical data

    At 0 day, 3 months, 6 months, 12 months, 18 months and 24 months of treatment

  • Enteric virus infection research

    At 0 day, 3 months, 6 months, 12 months, 18 months and 24 months of treatment

Study Arms (2)

1 - initiating treatment

10 patients with a genetic diagnosis of APDS type 1 or type 2 who are to be treated with a selective PI3Kδ inhibitor, Lenolisib.

Biological: Blood samplesBiological: Urine samplesBiological: Stool samples

2 - already on treatment

4 patients with a genetic diagnosis of APDS type 1 or type 2 already treated with a selective PI3Kδ inhibitor, Lenolisib.

Biological: Blood samplesBiological: Urine samplesBiological: Stool samples

Interventions

Blood samplesBIOLOGICAL

A maximum of 27 ml of blood collected at each visit for metabolic markers analysis

1 - initiating treatment2 - already on treatment
Urine samplesBIOLOGICAL

One urine sample collected at each visit for enteric virus infection research

1 - initiating treatment2 - already on treatment
Stool samplesBIOLOGICAL

One stool sample collected at each visit for enteric virus infection research

1 - initiating treatment2 - already on treatment

Eligibility Criteria

Age12 Years+
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Patients with genetic diagnosis of APDS type 1 or type 2 and planned to be treated by PI3Kδ selective inhibitor leniolisib and patients with genetic diagnosis of APDS type 1 or type 2 already treated by PI3Kδ selective inhibitor leniolisib in the last 2 years

You may qualify if:

  • Group 1:
  • Patients with genetic diagnosis of APDS type 1 or type 2 and planned to be treated by PI3Kδ selective inhibitor leniolisib
  • Primary immunodeficient patients with new disease-causing variants in the PIK3CD gene or PIK3R1 gene
  • Minimum age 12 years old
  • Patients or holders of parental authority do not oppose participation in this research.
  • Patients affiliated to a Health Insurance scheme or beneficiaries
  • Group 2 :
  • Patients with genetic diagnosis of APDS type 1 or type 2 already treated by PI3Kδ selective inhibitor leniolisib in the last 2 years
  • Patients whose pre-treatment samples are available/analyzable
  • Minimum age 12 years old
  • Patients or holders of parental authority do not oppose participation in this research.
  • patients affiliated to a Health Insurance scheme or beneficiaries

You may not qualify if:

  • Bone marrow transplantation
  • Refusal to participate to the study.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (7)

Hôpital Haut Levêque - BORDEAUX

Bordeaux, 33000, France

NOT YET RECRUITING

Hôpital Pellerin Enfants - BORDEAUX

Bordeaux, 33000, France

NOT YET RECRUITING

Hôpital Jeanne de Flandres - LILLE

Lille, 59000, France

RECRUITING

Hôpital La Timone adulte - MARSEILLE

Marseille, 13000, France

NOT YET RECRUITING

Hôpital Necker Enfants Malades - PARIS

Paris, 75015, France

RECRUITING

CHU IUCT Oncopole - TOULOUSE

Toulouse, France

NOT YET RECRUITING

Hôpital des enfants - TOULOUSE

Toulouse, France

NOT YET RECRUITING

Biospecimen

Retention: SAMPLES WITHOUT DNA

whole blood, urine, stool

MeSH Terms

Conditions

Activated PI3K-delta Syndrome

Interventions

Blood Specimen Collection

Intervention Hierarchy (Ancestors)

Specimen HandlingClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisPuncturesSurgical Procedures, OperativeInvestigative Techniques

Study Officials

  • Sven Kracker, PHD

    Institut National de la Santé Et de la Recherche Médicale, France

    STUDY DIRECTOR

Central Study Contacts

Michaela SEMERARO, MD, PhD

CONTACT

Laure CHOUPEAUX, MSc

CONTACT

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

November 15, 2024

First Posted

November 19, 2024

Study Start

June 26, 2025

Primary Completion (Estimated)

June 27, 2029

Study Completion (Estimated)

December 27, 2029

Last Updated

March 23, 2026

Record last verified: 2026-03

Data Sharing

IPD Sharing
Will not share

Locations