Sedentary or Exercised : Neuromuscular and Immunometabolic Observations and Retrospection

NCT06692543 | Recruiting

• 1 other identifier: 24-PP-07
• Study Type: interventional
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

The distinction between healthy and pathological ageing has given rise to the vitality capacity concept. Underlying the physiological aspect of ageing, vitality capacity is reflected in the neuromuscular function, metabolism, and immune and stress response. The three domains seem to deteriorate with age, except among individuals who have maintained physical activity throughout their lives. Furthermore, immune cells, particularly T lymphocytes, appear to play a key role in each dimension of vitality capacity, as well as in the mice ageing pathway. Investigators sought to determine the effect of lifelong physical activity on vitality capacity, and especially on T cell metabolism. In this optic, fourty healthy participants over the age of 55 will be allocated to two groups: 20 who have maintained physical activity for the past 30 years, and 20 who have not. Each participant's vitality capacity will be deeply tested and compared to their physical activity background.

Conditions

Aging

Keywords

immunity; aging; muscles; exercise; metabolism; physical activity

Outcome Measures

Primary Outcomes (2)

• Difference of T cell metabolism between active and inactive participants

  On the bloodwithdraw, level of T cells metabolism will be measured by using the SCENITH (Single Cell ENergetIc metabolism by profiling Translation inHibition) method

  at day 0

• Difference of T cell metabolism between active and inactive participants

  On the bloodwithdraw, level of T cells metabolism will be measured by RNA sequencing

  at day 0

Secondary Outcomes (3)

• WHOQOLD-OLD Questionnaire

  at day 0

• Capacity vitality

  at day 0 and 14

• Neuromuscular parameters

  at day 0 and 14

Study Arms (2)

Physically active

EXPERIMENTAL

Participants of this group are physically active subjects \- V1 will take place at the Nice University Hospital, in the morning, on an empty stomach. After signing the consent form, the participant will have a blood sample taken (24mL), answer questionnaire and have a body assessment by bone densitometry. At the end of the appointment , the participant will wear an accelerometer and fill in his or her food intake for one week. \- V2 (approximatly 2 weeks after V1), will take place at LAMHESS, the participant will perform neuromuscular tests on an ergometer, with high-density electromyography (HDEMG) sensors on the thighs.

Other: Active population

Physically inactive

EXPERIMENTAL

Participants of this group are physically inactive subjects \- V1 will take place at the Nice University Hospital, in the morning, on an empty stomach. After signing the consent form, the participant will have a blood sample taken (24mL), answer questionnaire and have a body assessment by bone densitometry. At the end of the appointment , the participant will wear an accelerometer and fill in his or her food intake for one week. \- V2 (approximatly 2 weeks after V1), will take place at LAMHESS, the participant will perform neuromuscular tests on an ergometer, with high-density electromyography (HDEMG) sensors on the thighs.

Other: Inactive population

Interventions

Active population OTHER

1 blood withdraw, questionnaire and tests

Physically active

Inactive population OTHER

1 blood withdraw, questionnaire and tests

Physically inactive

Eligibility Criteria

• Age: 55 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Men and women \> 55 years old
• Participants who are non-smokers or have been weaned for more than 3 years
• Menopausal women (confirmed for at least 6 months)
• Participants affiliated to the social security system
• Signature of informed consent
• Any person with structured aerobic physical activity (mainly involving cardiorespiratory function) on at least 3 days/week over the last 30 years without an interruption of more than 1 week in the 3 months preceding the study and without an interruption of more than 12 consecutive months over the last 30 years, as assessed by an interview conducted by an investigator specialising in physical activity;
• have a level of physical activity that meets the recommendations for physical activity in terms of the duration and intensity of weekly cardiorespiratory effort (WHO, 2020; ANSES, 2016) according to the criteria assessed by the GPAQ: at least 150 to 300 minutes per week of moderate-intensity aerobic activity (e.g., 5 days of moderate intensity activity of at least 30 minutes/day) or at least 75 to 150 minutes of sustained intensity endurance activity (e.g., 3 days of sustained activity of 25 minutes/day); or an equivalent combination of moderate and sustained intensity activities throughout the week.
• Any person who has or has had a low physical intensity professional activity over the last 30 years: sedentary professional activity (work time generally spent in a seated position, such as office work) and/or a standing professional activity (work time spent standing or walking, without intense physical effort (e.g. shop assistant, hairdresser, caretaker, etc.).
• Anyone who does not practise or has not practised sport, fitness or other physical leisure activities alone or under supervision over the last 30 years, or who practises or has practised, over the last 30 years, a sport, fitness or other physical leisure activity less than or equal to 2 days a week and for a weekly period not exceeding 75 minutes.
• Anyone whose active mode of transport (cycling, walking) does not exceed an overall duration of 75 minutes per week, and whose consecutive time slots per journey are less than 10 minutes.
• Person suffering from a chronic illness
• Anyone who has used performance-enhancing drugs
• Anyone with a disability
• Person on medication
• Recent hospitalisation (within the last 6 months)

Sponsors & Collaborators

• Centre Hospitalier Universitaire de Nice: lead
• LAMHESS: collaborator
• INSERM-C3M: collaborator
• Institut de bilogie de Valrose: collaborator
• LP2M: collaborator

Study Sites (1)

CHU de Nice

Nice, 06000, France

RECRUITING

MeSH Terms

Conditions

Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Study Officials

• Olivier GUERIN, MD

  Centre Hospitalier Universitaire de Nice

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Anne-Sophie Rousseau, PU

CONTACT

06,83,52,26,73; anne-sophie.rousseau@univ-cotedazur.fr

Frederic CHORIN, PhD

CONTACT

04 92 03 49 24; chorin.f@chu-nice.fr

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: 2x20 people aged \>55 yo, 1 group physically active, the other is physically inactive

Study Record Dates

• First Submitted: November 14, 2024
• First Posted: November 18, 2024
• Study Start: April 14, 2025
• Primary Completion (Estimated): April 1, 2028
• Study Completion (Estimated): December 1, 2028
• Last Updated: May 22, 2026

Record last verified: 2026-05

Data Sharing

• IPD Sharing: Will not share

Locations

FR (1)