Gut-muscle Axis: Exploring the Gut Microbiota Influences Host Energy Sources and Exercise Performance

NCT06670742 | Not Yet Recruiting

• 1 other identifier: NTSUIRB - 112 - 007
• Study Type: interventional
• Target: 90
• Locations: 0 countries
• Sites: N/A

Brief Summary

Through training, the investigators can adjust the composition and functions of gut microbiota. Exercise also alters the body's energy metabolism pathways, which, in turn, impacts the gut microbiota and its metabolic products. Understanding how the body utilizes and regulates energy during exercise is essential for enhancing athletic performance. This study aims to explore the relationships among post-exercise energy metabolites, gut microbiota, and their metabolic products, applying omics technologies in the field of sports science. This interdisciplinary research will contribute to fields such as exercise physiology, sports nutrition, exercise biochemistry, and sports medicine, providing innovative and practical research insights for advancing sports science in our country.

Conditions

Marathon Runner

Outcome Measures

Primary Outcomes (11)

• Concentration of lactate

  Measure changes in lactate concentration.

  The testing time points are set as follows: before exercise, at the 5-kilometer mark during exercise, immediately after exercise, and at 5, 15, 30, and 60 minutes post-exercise.

• Concentration of creatine kinase

  Measure changes in creatine kinase concentration.

  The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.

• Concentration of glucose

  Measure changes in glucose concentration.

  The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.

• Concentration of creatinine

  Measure changes in creatinine concentration.

  The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.

• Concentration of blood urea nitrogen

  Measure changes in blood urea nitrogen concentration.

  The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.

• Concentration of lactate dehydrogenase

  Measure changes in lactate dehydrogenase concentration.

  The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.

• Concentration of free fatty acid

  Measure changes in free fatty acid concentration.

  The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.

• Concentration of cortisol

  Measure changes in cortisol concentration.

  The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.

• ood frequency questionnaire(FFQ)

  The participants' diets were assessed using a semiquantitative food frequency questionnaire(FFQ). The FFQ used in this study was validated in a previous study; the questionnaire exhibited reliability and validity for identifying major nutrients in the diets of Taiwanese vegetarians and omnivores.(Chiu TH et al., 2014) In this study, the nutrient analysis programme used to calculate the results of the FFQ was based on Taiwan's Food Composition Database. To assess the intake frequency of each food item, frequencies ranged from "never" or "\<1 time per month" to "more than 2 times per day.

  Before exercise

• Gut microbiota analysis

  Collect participant stool samples for gut microbiota analysis.

  The testing time points are set as follows: before exercise, immediately after exercise, and at 24 and 48 hours post-exercise.

• Untargeted metabolome analysis

  Collect participant stool samples for metabolome analysis.Metabolite identification aims to analyze the metabolome composition of biological samples, investigate the regulation of metabolic pathways, and explore biochemical mechanisms.

  The testing time points are set as follows: before exercise, immediately after exercise, and at 24 and 48 hours post-exercise.

Secondary Outcomes (3)

• Body composition

  Before exercise

• Rate of Perceived Exertion(RPE)

  The testing time points are set as follows: before exercise, at the 5-kilometer mark during exercise, and immediately after exercise.

• Heart rate

  The testing time points are set as follows: before exercise, at the 5-kilometer mark during exercise, and immediately after exercise.

Study Arms (2)

Marathon running group

EXPERIMENTAL

Run 10 kilometers on a treadmill.

Device: running

General healthy adult group

NO INTERVENTION

No intervention will be conducted.

Interventions

running DEVICE

Running 10 kilometers on a treadmill

Marathon running group

Eligibility Criteria

• Age: 20 Years - 65 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Training frequency of at least 3 times per week in the past year, with each session involving at least 1 hour of running.
• Participation in at least 2 marathon events within the past 2 years, with a completion time of under 4 hours and 30 minutes for a full marathon, or under 2 hours and 15 minutes for a half marathon.

You may not qualify if:

• Excludes individuals with cardiovascular diseases, hypertension, epilepsy, bronchitis, severe asthma, heart disease, inflammatory bowel disease, bacterial or viral infections, disabilities, or chronic bone diseases.
• Habitual smokers.
• Individuals with highly irregular eating habits or sleep patterns due to shift work or night work.
• History of chronic neuro-muscular injuries or disorders in the past 6 months.
• Individuals who have received antibiotic treatment or taken probiotic supplements in the past 6 months.
• General Healthy Adult Group
• Generally healthy, untrained individuals.
• Excludes individuals with cardiovascular diseases, hypertension, epilepsy, bronchitis, severe asthma, heart disease, inflammatory bowel disease, bacterial or viral infections, disabilities, or chronic bone diseases.
• Habitual smokers.
• Individuals with highly irregular eating habits or sleep patterns due to shift work or night work.
• History of chronic neuro-muscular injuries or disorders in the past 6 months.
• Individuals who have received antibiotic treatment or taken probiotic supplements in the past 6 months.

Sponsors & Collaborators

• National Taiwan Sport University: lead

Study Officials

• Yi-Ju Hsu

  Associate Professor

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Yi-Ju Hsu

CONTACT

+886-3-328-3201 #2430; ruby780202@ntsu.edu.tw

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: October 26, 2024
• First Posted: November 1, 2024
• Study Start: November 8, 2024
• Primary Completion: December 31, 2024
• Study Completion: December 31, 2024
• Last Updated: November 1, 2024

Record last verified: 2024-10