Antibacterial Effect of Zinc Oxide Nanoparticles on Acinetobacter Baumannii Isolated from Patients with Hospital Acquired Infections in Sohag University Hospitals, Egypt

NCT06628076 | Not Yet Recruiting

• 1 other identifier: Soh-Med-24-9-1MD
• Study Type: observational
• Target: 150
• Locations: 1 country
• Sites: 1

Brief Summary

A. baumannii is known as the most frequently isolated organism in intensive care units (ICUs), causing a variety of nosocomial infections, including pneumonia, urinary tract infections (UTIs), bacteremia as well as skin and soft tissue infections. These infections are usually associated with high mortality rates ranging between 26% among hospitalized patients and 43% among ICU patients.

Conditions

Infections, Nosocomial; Infections, Respiratory Tract

Keywords

A. baumannii.; Biofilm; ZnO NPs; hospital aquired infections

Outcome Measures

Primary Outcomes (5)

• A. baumannii antibiotic susceptibility profile

  A. baumannii antibiotic susceptibility profile will be done using modified kirbybeur method

  October 2024 to December 2025

• Isolation and identification of A. baumannii from different clinical samples

  Isolation and identification of A. baumannii from different clinical samples using MacConKey medium, oxidase test. TSI, And automated identification ViteK system

  October 2024 to December 2025

• A. baumannii strains which produce biofilm

  Detection of A. baumannii strains which produce biofilm using tissue culture plates and Gram stain

  October 2024 to December 2025

• Ability of ZnO NPs to inhibit the phenomenon of biofilm formation by MDR A. baumannii strains

  Assessment of ability of ZnO NPs to inhibit the phenomenon of biofilm formation by MDR A. baumannii strains using ZnO NPs to be applied by different concentrations on tissue culture plates and measure the degree of Biofilm formation using ELISA

  October 2024 to December 2025

• The effect of ZnO NPs on the expression of some efflux pump genes and biofilm related genes in MDR A. baumannii strains

  Evaluation the effect of ZnO NPs on the expression of some efflux pump genes and biofilm related genes in MDR A. baumannii strains using real time PCR(Polymerase Chain reaction)

  October 2024 to December 2025

Interventions

Culture specific antibiotic therapy DIAGNOSTIC_TEST

Samples will be cultured on MacConkey agar. Morphological identifications of growth isolates by Gram staining, colony features and conventional biochemical tests. All isolates will be identified to species level using automated bacterial identification systems.

Antibiotic DIAGNOSTIC_TEST

Strains confirmed as A. baumannii will be examined for their different antibiotic susceptibility by modified Kirby Bauer\'s disc diffusion method on Mueller Hinton Agar.

Biofilm assessment DIAGNOSTIC_TEST

The biofilm formation activity of A. baumannii isolates will be tested using the microtitre plate technique

nanoparticle zinc oxide DIAGNOSTIC_TEST

Detection of the effect of ZnO NPs on the biofilm producer MDR A. baumannii strains using the same method

Molecular detection of antibiotic resistance DIAGNOSTIC_TEST

\- Evaluation the effect of ZnO NPs on the expression of some efflux pump genes, and biofilm related genes in MDR A. baumannii isolates using Real time PCR technology.

Eligibility Criteria

• Age: Up to 90 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

This study will be carried out at Department of Medical Microbiology and Immunology, Sohag Faculty of Medicine and Sohag University Hospitals.

You may qualify if:

• All patients suffering from infections that can be caused by A. baumannii.

You may not qualify if:

• All patients suffering from infections that aren\'t caused by A. baumannii.

Sponsors & Collaborators

• Sohag University: lead

Study Sites (1)

Sohag university, Faculty of medicine

Sohag, 534568, Egypt

Location

Related Links

• Related Info

Biospecimen

Retention: SAMPLES WITH DNA

Different samples (pus, urine, urinary catheter samples, wound swabs, sputum, bronchoalveolar lavage and central venous catheter samples) will be collected under strict aseptic precautions from patients admitted at different departments at Sohag university hospitals

MeSH Terms

Conditions

Cross Infection; Respiratory Tract Infections

Condition Hierarchy (Ancestors)

Infections; Iatrogenic Disease; Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms; Respiratory Tract Diseases

Central Study Contacts

Shimaa Anwar Ahmed, Assistant Lecturer

CONTACT

+201092533978; shaymaa_abdelgaber@med.sohag.edu.eg

Noha Saber Shafik, lecturer

CONTACT

Nohasaber@med.sohag.edu.eg

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant lecturer of Medical Microbiology and Immunology

Study Record Dates

• First Submitted: September 26, 2024
• First Posted: October 4, 2024
• Study Start: October 10, 2024
• Primary Completion: October 10, 2024
• Study Completion: December 30, 2025
• Last Updated: October 4, 2024

Record last verified: 2024-10

Data Sharing

• IPD Sharing: Will not share

Locations

EG (1)