Mechanisms of Anabolic Resistance in Older Humans

NCT06617195 | Recruiting

• 1 other identifier: 24-003329
• Study Type: observational
• Target: 120
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this research is to understand how chronic inflammation affects muscle function and responses to exercise in older adults.

Conditions

Aging

Outcome Measures

Primary Outcomes (1)

• Anabolic response

  Skeletal muscle protein synthesis rates will be measured from isotopic enrichment of muscle proteins

  2 months

Study Arms (2)

Young

Men and women between the ages of 21-35 years will be recruited for this study. All participants will complete an outpatient study day (body composition, blood draw, treadmill test, strength test) and an inpatient study day (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).

Other: Exercise

Older

Men and women between the ages of 70-85 years will be recruited for this study. All participants will complete an outpatient study day (body composition, blood draw, treadmill test, strength test) and an inpatient study day (muscle biopsies, fat biopsies, indirect calorimetry, exercise test).

Other: Exercise

Interventions

Exercise OTHER

Young and older adults will perform a single bout of unaccustomed resistance exercise to evaluate molecular and cellular response in skeletal muscle

Older; Young

Eligibility Criteria

• Age: 21 Years - 85 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

Men and women age 21-35 years or 70-85 years of age

You may not qualify if:

• Diabetes or fasting plasma glucose 126 mg/dL
• Body mass index (BMI) 30 kg/m2
• Anemia (female subjects hemoglobin of \<11 g/dl and male subjects hemoglobin \<12 g/dl)
• Active coronary artery disease or history of unstable macrovascular disease (unstable angina, myocardial infarction, stroke, and revascularization of coronary, peripheral or carotid artery within 3 months of recruitment)
• Renal failure (serum creatinine \> 1.5mg/dl)
• Chronic active liver disease (AST\>144IU/L or ALT\>165IU/L)
• Oral warfarin group medications or history of blood clotting disorders.
• Smoking
• Pregnancy or breastfeeding
• Alcohol consumption greater than 2 glasses/day or other substance abuse
• Untreated or uncontrolled hypothyroidism
• Debilitating chronic disease (at the discretion of the investigators)

Sponsors & Collaborators

• Mayo Clinic: lead

Study Sites (1)

Mayo Clinic

Rochester, Minnesota, 55905, United States

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

Plasma Serum Adipose tissue Skeletal muscle tissue

MeSH Terms

Interventions

Exercise

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Ian Lanza

  Mayo Clinic

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Rachel Passehl

CONTACT

507-255-8112; passehl.rachel@mayo.edu

Ian Lanza

CONTACT

lanza.ian@mayo.edu

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: September 13, 2024
• First Posted: September 27, 2024
• Study Start: January 14, 2025
• Primary Completion (Estimated): September 16, 2028
• Study Completion (Estimated): September 16, 2030
• Last Updated: January 21, 2026

Record last verified: 2026-01

Data Sharing

• IPD Sharing: Will share

Locations

US (1)