Relationship Between Oxidative Stress and Aortic Stiffness

NCT06590792 | Completed

• 1 other identifier: Soccer aortic stiffness
• Study Type: interventional
• Target: 43
• Locations: 1 country
• Sites: 2

Brief Summary

The goal of this clinical trial is to search about if there is any relationship between the aortic stiffness and oxidative stress response in adolescent football players. The main questions are;

• Is there any difference between the adolescent football players and healthy sedentary control group in terms of oxidative stress and aortic stiffness parameters?
• Is there any relationship between the aortic stiffness and oxidative response to acute exercise training in adolescent male football players? Participants will perform acute exhaustive exercise training. Venous blood will be drained before and after exercise training. Oxidative parameters will be measured.

Conditions

Aortic Stiffness; Oxidative Stress; Football Player

Keywords

stiffness; elastography; football; oxidative stress

Outcome Measures

Primary Outcomes (10)

• aortic strain (%)

  =\[(aortic systolic diameter - diastolic diameter)× 100\] / aortic diastolic diameter

  will be measured twice before and after acute exhaustive exercise, through study completion, up to 8 weeks

• aortic stiffness index

  = ln (systolic pressure / diastolic pressure) / aortic strain

  will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks

• aortic distensibility (cm2.dyn-1.10-6)

  = (2 × aortic strain) / (systolic pressure-diastolic pressure)

  will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks

• serum lipid hydroperoxide assay

  lipid hydroperoxide (LHP) level of serum (nmol/mL). Lipid hydroperoxide (LOOH) is the first, comparatively stable, product of the lipid peroxidation reaction.

  will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks

• serum malondialdehide assay

  serum malondialdehide (MDA) level of serum (nmol/mL).MDA is the end product of the oxidation of polyunsaturated fatty acids.

  will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks

• serum advanced oxidation protein products assay

  Advanced oxidation protein products (AOPP) level of serum (mikromol/L ). AOPP are the dityrosine-containing and crosslinking protein products formed during oxidative stress by reaction of plasma protein with chlorinated oxidants, and often carried by albumin in vivo

  will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks

• serum advanced glycation end products assay

  serum advanced glycation end products (AGE) level of serum(FU/mg). AGEs are are a heterogeneous group of proteins and lipids, formed by nonenzymatic glycation after persistent contact with aldose sugars

  will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks

• serum ferric reducing antioxidant power assay

  ferric reducing antioxidant power (FRAP) level of serum (mikromol/mL ). FRAP is an antioxidant capacity assay that uses Trolox as a standard

  will be measured twice before and after acute exhaustive exercise, through study completion, up to 8 weeks

• serum superoxide dismutase activity assay

  copper-zinc superoxide dismutase activity (SOD) level of serum (U/mL). SODs are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide

  will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks

• serum total antioxidant status and total oxidant status assay

  total antioxidant status (TAS) and total oxidant status (TOS) levels of serum (trolox equivalent/L) are two indicators to determine all antioxidants or oxidants in samples, which can more comprehensively reflect the changes of oxidant and antioxidant capacity of samples

  will be measured twice before and after acute exhaustive exercise,through study completion, up to 8 weeks

Study Arms (2)

football player

EXPERIMENTAL

14-18 years old male football players who play in the infrastructure of a football club

Other: exhaustive exercise training session

healthy control group

ACTIVE COMPARATOR

14-18 years old male healthy participants who don't involve any regular sports training

Other: exhaustive exercise training session

Interventions

exhaustive exercise training session OTHER

Cardiovascular exercise test will be performed by the participants. This test is sustained until the participants feel exhausted.

football player; healthy control group

Eligibility Criteria

• Age: 14 Years - 18 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64)

You may qualify if:

• age between 14-18
• male gender
• football player
• attending regular football training for at least 1 season

You may not qualify if:

• female gender
• attending any other sports training except from football
• having any chronic disease
• having any musculoskeletal disease which limits to join exhaustive exercise training

Sponsors & Collaborators

• Istanbul University: lead

Study Sites (2)

Istanbul Faculty of Medicine

Istanbul, 34093, Turkey (Türkiye)

Location

Şensu Dinçer

Istanbul, 34093, Turkey (Türkiye)

Location

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: DOUBLE
• Who Masked: INVESTIGATOR, OUTCOMES ASSESSOR
• Masking Details: Echocardiographer and biochemistry expert will be blind to participants type (sedentary or football player)
• Purpose: SCREENING
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: medical doctor

Model Details: Two groups including 20 participants in each of them will be involved the study. One group will consist of adolescent football players, while the other group will consist of non-athlete healthy participants

Study Record Dates

• First Submitted: September 4, 2024
• First Posted: September 19, 2024
• Study Start: May 30, 2025
• Primary Completion: June 30, 2025
• Study Completion: July 15, 2025
• Last Updated: August 11, 2025

Record last verified: 2025-08

Locations

TU (2)