NCT06571149

Brief Summary

DO-IT is an international, multicenter, prospective, two-arm, randomized, open label, blinded endpoint superiority trial determining the safety and efficacy of intravenous thrombolysis (IVT) in participants experiencing an acute ischemic stroke (AIS) with recent (within the last 48 hours) intake of direct oral anticoagulant (DOAC). For this purpose, 906 adult participants experiencing an AIS with recent DOAC intake will be enrolled at several high-volume international stroke centers and randomly assigned in a ratio of 1:1 to one of two treatment arms: (1) IVT and standard of care/best medical treatment or (2) standard of care/best medical treatment. The DO-IT trial is a definitive test of the hypothesis that IVT is superior to standard of care for achieving better outcome at 90 days in AIS participants with recent DOAC intake.

Trial Health

88
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Strong global presence with extensive site network
Enrollment
906

participants targeted

Target at P75+ for phase_4

Timeline
33mo left

Started Mar 2025

Longer than P75 for phase_4

Geographic Reach
12 countries

102 active sites

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress34%
Mar 2025Apr 2029

First Submitted

Initial submission to the registry

August 22, 2024

Completed
4 days until next milestone

First Posted

Study publicly available on registry

August 26, 2024

Completed
7 months until next milestone

Study Start

First participant enrolled

March 14, 2025

Completed
3.9 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 31, 2029

Expected
3 months until next milestone

Study Completion

Last participant's last visit for all outcomes

April 30, 2029

Last Updated

August 3, 2026

Status Verified

July 1, 2026

Enrollment Period

3.9 years

First QC Date

August 22, 2024

Last Update Submit

July 30, 2026

Conditions

Keywords

DOACNOAC (Novel Oral Anticoagulants)Anticoagulationr-tPA (tissue-type plasminogen activator)AlteplaseTenecteplaseRivaroxabanApixabanDabigatranEdoxaban

Outcome Measures

Primary Outcomes (1)

  • Modified Rankin Scale (mRS)

    Modified Rankin Scale is a scale that runs from 0-6, running from perfect health without symptoms (0) to death (6).

    90 days +/- 2 weeks after randomization

Secondary Outcomes (3)

  • Dichotomized modified Rankin Scale (mRS) 0-2 (good functional outcome)

    90 days +/- 2 weeks post-randomization

  • Change from baseline in stroke severity (National Institutes of Health Stroke Scale, NIHSS)

    24 +/- 12 hours after Intervention

  • Health-related Quality of Life (EuroQol 5D-3L questionnaire)

    90 days +/- 2 weeks post-randomization

Other Outcomes (3)

  • Rate of Symptomatic intracranial hemorrhage

    Within the first 36 hours post-randomization

  • Rate of Major extracranial bleeding

    Within the first 24 hours post-randomization

  • All-cause mortality

    Up to 7 to 10 days after admission or until discharge, usually 2 weeks after admission

Study Arms (2)

Best Medical Treatment (standard of care)

NO INTERVENTION

Patients will receive Standard of care/Best medical treatment according to local applicable guidelines, including the current American Heart Association/American Stroke Association (AHA/ASA) and European Stroke Organisation/ European Society of Minimally Invasive Neurological Therapy (ESO/ESMINT) guidelines.

Intravenous Thrombolysis + Best medical treatment (standard of care)

EXPERIMENTAL

Patients will receive intravenous administration of Tenecteplase or Alteplase.

Drug: Tenecteplase or Alteplase

Interventions

Patients randomized to intravenous thrombolysis (IVT) treatment will receive intravenous Tenecteplase or Alteplase.

Intravenous Thrombolysis + Best medical treatment (standard of care)

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Informed consent (deferred consent when possible according to national legislation)
  • AIS eligible to receive intravenous alteplase/tenecteplase as per standard of care disabling according to the judgement of the treating physician
  • DOAC ingestion within 48 hours prior to enrollment, or patient with an ongoing prescription of DOAC but exact time point of last intake is unknown.
  • Either
  • Can be randomized within 4 hours 15 minutes and treated within 4 hours 30 minutes of last known well time OR
  • MRI showing a pattern of "DWI-FLAIR-mismatch", i.e. acute ischemic lesion visibly on DWI ("positive DWI") but no marked parenchymal hyperintensity visible on FLAIR ("negative FLAIR") indicative of an acute ischemic lesion ≤4.5 hours of age AND Treatment can be started within 4.5 hours of symptom recognition (e.g., awakening).

You may not qualify if:

  • Contra-indications to IVT by the current standard of care of the treating physicians with the exception of recent DOAC intake as specified above.
  • Intended reversal by specific or unspecific reversal agents
  • Pregnancy or lactating women. To be reasonable sure to exclude women with ongoing pregnancy, women are not considered of childbearing potential if they fulfill the following criteria
  • Age \> 55 years OR
  • Age \< 55 years and at least 12 months since last menstrual period OR
  • Have had a documented surgical sterilization
  • Patient \< 18 years of age (since the benefit of IVT is unproven in this population)
  • Intended treatment with endovascular reperfusion strategies

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (102)

Barmherzige Brüder Krankenhaus Eisenstadt

Eisenstadt, 7000, Austria

RECRUITING

Universitätsklinik für Neurologie Graz

Graz, 8036, Austria

RECRUITING

Medical University of Innsbruck

Innsbruck, 6020, Austria

NOT YET RECRUITING

Christian Doppler Klinik

Salzburg, 5020, Austria

RECRUITING

Barmherzige Brüder Krankenhaus Wien

Vienna, 1020, Austria

RECRUITING

Medizinische Universität Wien

Vienna, 1090, Austria

RECRUITING

AZ Sint-Jan Brugge AV

Bruges, Belgium

RECRUITING

Cliniques Universitaires de Bruxelles

Brussels, Belgium

RECRUITING

UCL St Luc

Brussels, Belgium

RECRUITING

Hôpital Civil Marie Curie

Charleroi, Belgium

RECRUITING

University Hospital Gent

Ghent, Belgium

RECRUITING

AZ Groeninge, Campus Kennedy

Kortrijk, Belgium

RECRUITING

UZ Leuven

Leuven, 3000, Belgium

RECRUITING

CHU de Liège

Liège, 4000, Belgium

NOT YET RECRUITING

Hamilton Health Sciences

Hamilton, Ontario, L8L 2X2, Canada

NOT YET RECRUITING

Helsinki University Central Hospital

Helsinki, 00290, Finland

RECRUITING

CHU Bordeaux

Bordeaux, 33000, France

NOT YET RECRUITING

Chu Grenoble Alpes

Grenoble, 38043, France

NOT YET RECRUITING

CHU de Lille

Lille, 59037, France

NOT YET RECRUITING

Hôpital Pierre Wertheimer

Lyon, 69677, France

NOT YET RECRUITING

CHU Montpellier

Montpellier, 34 295, France

NOT YET RECRUITING

CHU Nancy

Nancy, 54035, France

NOT YET RECRUITING

AP-HP, Pitié-Salpêtrière

Paris, 75013, France

NOT YET RECRUITING

GHU Paris Psychiatrie et Neurosciences, Sainte Anne

Paris, 75014, France

RECRUITING

Hôpital Paris Saint-Joseph

Paris, 75014, France

NOT YET RECRUITING

AP-HP, Lariboisière

Paris, 75019, France

NOT YET RECRUITING

Perpignan Hospital Center

Perpignan, 66000, France

NOT YET RECRUITING

Hôpital de Hautepierre

Strasbourg, 67098, France

NOT YET RECRUITING

Hôpital FOCH

Suresnes, 92150, France

NOT YET RECRUITING

SRH Klinikum Karlsbad-Langensteinbach GmbH

Karlsbad, Baden-Wurttemberg, 76307, Germany

RECRUITING

RHÖN-KLINIKUM AG - Campus Bad Neustadt

Bad Neustadt an der Saale, 97616, Germany

RECRUITING

Sozialstiftung Bamberg

Bamberg, 96049, Germany

NOT YET RECRUITING

St. Josef-Hospital

Bochum, 44791, Germany

RECRUITING

Knappschaft Kliniken Bottrop GmbH

Bottrop, 46242, Germany

RECRUITING

Helios Klinikum Erfurt GmbH

Erfurt, 99089, Germany

RECRUITING

Alfried Krupp Krankenhaus

Essen, 45131, Germany

NOT YET RECRUITING

Universitätsmedizin Frankfurt

Frankfurt, 60590, Germany

RECRUITING

Universitätsklinikum Freiburg

Freiburg im Breisgau, 79106, Germany

RECRUITING

Universitätsklinikum Gießen und Marburg GmbH

Giessen, Germany

RECRUITING

Universitätsmedizin Göttingen, Abteilung Neurologie

Göttingen, Germany

RECRUITING

Asklepios Klinik Barmbek

Hamburg, 22307, Germany

NOT YET RECRUITING

Asklepios Klinik Wandsbek

Hamburg, Germany

RECRUITING

Heidelberg University Hospital

Heidelberg, 69120, Germany

RECRUITING

Universitätsklinikum des Saarlandes

Homburg, 66421, Germany

NOT YET RECRUITING

Universitätsklinikum Jena

Jena, 07747, Germany

RECRUITING

Klinikum Kassel

Kassel, 34125, Germany

RECRUITING

Universität Leipzig

Leipzig, 04103, Germany

RECRUITING

Klinikum der Stadt Ludwigshafen

Ludwigshafen, Germany

RECRUITING

Sana Kliniken Lübeck GmbH

Lübeck, 23560, Germany

NOT YET RECRUITING

Klinikum der Universität München

München, 81377, Germany

RECRUITING

RoMed Klinikum Rosenheim Klinik

Rosenheim, 83022, Germany

NOT YET RECRUITING

Nordwest-Krankenhaus Sanderbusch

Sande, 26452, Germany

RECRUITING

Helios Klinik Schleswig GmbH, Klinik für Neurologie und Neurologische

Schleswig, Germany

RECRUITING

Universitätsklinikum Tübingen, Abt. Neurologie mit Schwerpunkt neurovaskuläre Erkrankungen

Tübingen, Germany

RECRUITING

Universitätsklinikum Ulm

Ulm, 89081, Germany

RECRUITING

Department of Neurology University Hospital of Alexandroupolis Democritus University of Thrace

Alexandroupoli, 68100, Greece

NOT YET RECRUITING

Eginition Hospital

Athens, 11528, Greece

NOT YET RECRUITING

"Attikon" University Hospital

Athens, 12462, Greece

NOT YET RECRUITING

Department of Neurology, School of Medicine University of Crete

Heraklion, 71500, Greece

NOT YET RECRUITING

University General Hospital of Patras

Pátrai, 265 04, Greece

NOT YET RECRUITING

2nd Department of Neurology, School of Medicine Faculty of Health Sciences, Aristotle University of Thessaloniki

Thessaloniki, 546 36, Greece

NOT YET RECRUITING

OLVG location East

Amsterdam, 1091, Netherlands

NOT YET RECRUITING

Academic Medical Center Amsterdam, Department of Neurology

Amsterdam, 1105, Netherlands

RECRUITING

Rijnstate

Arnhem, 6815, Netherlands

RECRUITING

Amphia Hospital

Breda, 4818, Netherlands

RECRUITING

Albert Schweitzer ziekenhuis

Dordrecht, 3318, Netherlands

NOT YET RECRUITING

Catharina Ziekenhuis

Eindhoven, 5623, Netherlands

NOT YET RECRUITING

University Medical Center Groningen

Groningen, 9700, Netherlands

RECRUITING

University Medical Center

Leiden, 2333, Netherlands

RECRUITING

University Medical Center

Maastricht, 6229, Netherlands

NOT YET RECRUITING

St. Antonius Ziekenhuis

Nieuwegein, 3435, Netherlands

NOT YET RECRUITING

Radboud University Nijmegen Medical Center

Nijmegen, 6500, Netherlands

RECRUITING

Erasmus Medical Center

Rotterdam, 3015, Netherlands

NOT YET RECRUITING

Franciscus Gasthuis

Rotterdam, 3045, Netherlands

NOT YET RECRUITING

Haaglanden MC, HMC Westeinde

The Hague, 2512, Netherlands

RECRUITING

Haga Ziekenhuis

The Hague, 6815, Netherlands

RECRUITING

University Medical Center Utrecht

Utrecht, 3584, Netherlands

RECRUITING

Isala Hospital

Zwolle, 8025, Netherlands

NOT YET RECRUITING

Akershus Hospital

Oslo, Norway

RECRUITING

Hospital Amadora-Sintra Fernando Fonseca

Amadora, 2720-276, Portugal

NOT YET RECRUITING

Hospitais da Universidade de Coimbra

Coimbra, 3004-561, Portugal

NOT YET RECRUITING

Hospital Dr. Nélio Mendonça

Funchal, 9000-177, Portugal

RECRUITING

Lisbon Central University Hospital Centre

Lisbon, 1150-199, Portugal

RECRUITING

Hospital Egas Moinz

Lisbon, 1449-005, Portugal

RECRUITING

Hospital Pedro Hispano

Matosinhos Municipality, 4464-513, Portugal

RECRUITING

Hospital de Santo António

Porto, 4099-001, Portugal

RECRUITING

ULS de Entre Douro e Vouga

Porto, 4520-211, Portugal

RECRUITING

Vall d'Hebron Stroke Center

Barcelona, 08035, Spain

NOT YET RECRUITING

Kantonsspital Baden AG

Baden, 5404, Switzerland

RECRUITING

University Hospital Basel

Basel, 4031, Switzerland

RECRUITING

Inselspital Bern

Bern, 3010, Switzerland

RECRUITING

MEDIN La Tour

Biel, 2502, Switzerland

RECRUITING

Hôpitaux Universitaires Genève

Geneva, 1205, Switzerland

RECRUITING

Médecin Associé Service de Neurologie Département des Neurosciences Cliniques CHUV, BH13

Lausanne, 1011, Switzerland

RECRUITING

Luzerner Kantonsspital

Lucerne, 6000, Switzerland

RECRUITING

Ente Ospedaliero Cantonale Istituto di Neuroscienze Cliniche della Svizzera Italiana Stroke Center EOC, Clinica di Neurologia c/o Ospedale Regionale di Lugano, sede Civico

Lugano, 6903, Switzerland

NOT YET RECRUITING

Neurologue FMH, AFC EEG/ENMG

Nyon, 1260, Switzerland

RECRUITING

Hospital de São João

Porto, 4200-319, Switzerland

RECRUITING

Kantonsspital St. Gallen

Sankt Gallen, Switzerland

RECRUITING

Klinik Hirslanden

Zurich, 8032, Switzerland

NOT YET RECRUITING

Stadtspital Zürich

Zurich, 8063, Switzerland

RECRUITING

Universitätsspital Zürich

Zurich, 8091, Switzerland

RECRUITING

MeSH Terms

Conditions

Ischemic Stroke

Interventions

TenecteplaseTissue Plasminogen Activator

Condition Hierarchy (Ancestors)

StrokeCerebrovascular DisordersBrain DiseasesCentral Nervous System DiseasesNervous System DiseasesVascular DiseasesCardiovascular Diseases

Intervention Hierarchy (Ancestors)

Serine EndopeptidasesEndopeptidasesPeptide HydrolasesHydrolasesEnzymesEnzymes and CoenzymesSerine ProteasesPlasminogen ActivatorsBlood Coagulation FactorsBlood ProteinsProteinsAmino Acids, Peptides, and ProteinsBiological Factors

Study Officials

  • Thomas Meinel, MD, PhD

    Insel Gruppe AG, University Hospital Bern

    STUDY DIRECTOR

Central Study Contacts

Thomas Meinel, MD, PhD

CONTACT

Freschta Zipser-Mohammadzada, PhD

CONTACT

Study Design

Study Type
interventional
Phase
phase 4
Allocation
RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

August 22, 2024

First Posted

August 26, 2024

Study Start

March 14, 2025

Primary Completion (Estimated)

January 31, 2029

Study Completion (Estimated)

April 30, 2029

Last Updated

August 3, 2026

Record last verified: 2026-07

Data Sharing

IPD Sharing
Will share

IPDMA (individual participant data meta-analysis) of similar interventional clinical studies is planned.

Shared Documents
STUDY PROTOCOL, ICF
Time Frame
After publication of primary study results
Access Criteria
Ethics clearance, agreement with the DO-IT collaboration about terms of use and authorship

Locations