Buffering Capacity of Matcha Green Tea on Salivary PH After an Acidic Attack

NCT06562699 | Not Yet Recruiting

• 1 other identifier: BC_MGT
• Study Type: interventional
• Target: 63
• Locations: 0 countries
• Sites: N/A

Brief Summary

This study is conducted to evaluate the buffering capacity of hot matcha brew in comparison with cold matcha brew and water on salivary pH after an acidic attack using a digital pH meter. Research Question: Will matcha green tea beverage have similar salivary buffering effect as water after an acidic challenge on dental interns? Steps in short

1. 1.Recruitment of the patients and clinical examination with medical and dental history taking.
2. 2.Informed consent taking for the eligible participants to participate in the study.
3. 3.A volume of 2 mL saliva samples will be collected before the commencement of acidic attack, baseline (T0).
4. 4.After the acidic attack, saliva will be collected again by spitting method and their pH value will be measured (T1).
5. 5.After 5 minutes of the acidic attack, participants will be instructed to swish either hot matcha tea, cold matcha tea, or water then swallow, and saliva samples will be collected (T2).
6. 6.Then saliva will be collected after 10 (T3), 20 (T4), 30 (T5), and 40 (T6) minutes.

Conditions

Diet Habit

Outcome Measures

Primary Outcomes (1)

• Salivary pH buffering effect

  PH will be assessed by a digital pH meter (AD1030, Adwa Instruments, Hungary). Calibration of the pH meter will be performed before measuring. The glass electrode will be immersed in the saliva sample, and after each measurement, it will be carefully cleaned with deionized water and dried with filter paper. The salivary pH value will be determined by 3 consecutive immersions of the pH meter glass electrode in the test tube with the sample, and the mean value will be taken as the obtained value. Measurements will be taken at baseline, 5 mins after the acidic attack, 5, 10, 20, 30, and 40 minutes after consumption of the buffering beverages.

  60 minutes

Study Arms (3)

Group A

EXPERIMENTAL

21 participants. They will be generated using numbers from 1 to 63, divided into three groups denoting with letters A, B, and C. Randomization will be generated using (www.randomization.com).

Other: Hot Matcha brew

Group B

EXPERIMENTAL

21 participants. They will be generated using numbers from 1 to 63, divided into three groups denoting with letters A, B, and C. Randomization will be generated using (www.randomization.com).

Other: Cold matcha brew

Group C

ACTIVE COMPARATOR

21 participants. They will be generated using numbers from 1 to 63, divided into three groups denoting with letters A, B, and C. Randomization will be generated using (www.randomization.com).

Other: Water

Interventions

Hot Matcha brew OTHER

* Hot matcha brew will be prepared in usucha (thin consistency) version-2 grams of matcha powder (Dr.Baby) mixed with 30 mL of hot water (75 ̊C). The first step will be to massage the matcha powder with a small amount of water until a smooth paste forms, then additional 50 mL of hot water will be added to dilute down the concentration. The matcha will then be vigorously shaken in a lidded jar. * After 5 minutes of the acidic attack, participants will be instructed to swish 25 mL of matcha tea 34 times then swallow, and saliva samples will be collected (T2). * Then saliva will be collected after 10 (T3), 20 (T4), 30 (T5), and 40 (T6) minutes.

Group A

Water OTHER

Water (elano) at 13 ̊C swished 34 times then swallowed.

Group C

Cold matcha brew OTHER

25 mL of cold matcha brew prepared in usucha (thin consistency) version-2 grams of matcha powder (Dr.Baby) mixed with 80 mL of cold water (13 ̊C) swished 34 times then swallowed.

Group B

Eligibility Criteria

• Age: 21 Years - 25 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Low caries-risk.
• Medically healthy.
• Have full permanent dentition.
• Practice daily oral hygiene habits.

You may not qualify if:

• Subjects who have active oral diseases as caries, periodontitis, gingivitis, or pulpitis.
• Subjects who have oral-related pain as sensitivity, ulcers, or infection.
• Subjects undergoing restorative or orthodontic treatment.
• Subjects with salivary gland diseases.
• Subjects with systemic diseases.
• Subjects on medications that can affect the salivary gland function (antihistamines, anticancer drugs) in a period of at least two months before the start of the trial.
• Subjects using antibiotics at the time of study or in the period of the last 15 days prior to the study.
• Mouth breathing.
• Smoking.
• Pregnancy.
• Subjects who did not give informed consent.

Sponsors & Collaborators

• NewGiza University: lead

MeSH Terms

Conditions

Feeding Behavior

Interventions

Water

Condition Hierarchy (Ancestors)

Behavior, Animal; Behavior

Intervention Hierarchy (Ancestors)

Hydroxides; Alkalies; Inorganic Chemicals; Anions; Ions; Electrolytes; Oxides; Oxygen Compounds

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: OUTCOMES ASSESSOR
• Purpose: PREVENTION
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant lecturer

Study Record Dates

• First Submitted: August 11, 2024
• First Posted: August 20, 2024
• Study Start: September 1, 2024
• Primary Completion: October 1, 2024
• Study Completion: December 1, 2024
• Last Updated: August 20, 2024

Record last verified: 2024-08