Interleukin-8 Level in Packed Red Blood Cells

NCT06530160 | Not Yet Recruiting

• 1 other identifier: Interleukin-8 in packed Rbcs
• Study Type: observational
• Target: 82
• Locations: 0 countries
• Sites: N/A

Brief Summary

1. 1.To compare level of interleukin-8 between leukodepleted and non-leukodepleted packed red blood cells.
2. 2.To show the effect of filtration of packed red blood cells on the level of inflammatory interleukin-8.

Conditions

Packed Red Blood Cells

Keywords

Interleukin-8

Outcome Measures

Primary Outcomes (1)

• Compare level of interleukin-8 between leukodepleted and non-leukodepleted packed red blood cells

  Show the effect of filteration of packed red blood cells on the level of inflammatory interleukin-8

  Baseline

Interventions

ELISA OTHER

Enzyme-linked immunosorbent assay

Eligibility Criteria

• Age: 18 Years - 65 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Healthy donors

You may qualify if:

• In total, 82 donors fulfilling all general criteria for blood donation, different blood groups and socioeconomic backgrounds, males and females belonging to this geographical area.

You may not qualify if:

• Donors not fulfilling all general criteria for blood donation according to World Health Organization:
• Age between 18 and 65
• Weight at least 50 kg
• Haemoglobin not less than 13 g/dl in males and 12 g/dl in females
• must be in good health at the time of donation
• Cannot donate if have a cold, flu, sore throat, cold sore, stomach bug or any other infection.
• Have ever had a positive test for HIV (AIDS virus)
• It is not advisable to donate blood while Breast-feeding

Sponsors & Collaborators

• Assiut University: lead

Related Publications (5)

• Anaya JM, Shoenfeld Y, Rojas-Villarraga A, Levy RA, Cervera R, editors. Autoimmunity: From Bench to Bedside [Internet]. Bogota (Colombia): El Rosario University Press; 2013 Jul 18. Available from http://www.ncbi.nlm.nih.gov/books/NBK459447/

  PMID: 29087650 BACKGROUND

• Turner MD, Nedjai B, Hurst T, Pennington DJ. Cytokines and chemokines: At the crossroads of cell signalling and inflammatory disease. Biochim Biophys Acta. 2014 Nov;1843(11):2563-2582. doi: 10.1016/j.bbamcr.2014.05.014. Epub 2014 Jun 2.

  PMID: 24892271 BACKGROUND

• Darbonne WC, Rice GC, Mohler MA, Apple T, Hebert CA, Valente AJ, Baker JB. Red blood cells are a sink for interleukin 8, a leukocyte chemotaxin. J Clin Invest. 1991 Oct;88(4):1362-9. doi: 10.1172/JCI115442.

  PMID: 1918386 BACKGROUND

• Davenport RD, Strieter RM, Standiford TJ, Kunkel SL. Interleukin-8 production in red blood cell incompatibility. Blood. 1990 Dec 15;76(12):2439-42.

  PMID: 1702325 BACKGROUND

• Shukla R, Patel T, Gupte S. Release of cytokines in stored whole blood and red cell concentrate: Effect of leukoreduction. Asian J Transfus Sci. 2015 Jul-Dec;9(2):145-9. doi: 10.4103/0973-6247.162708.

  PMID: 26420933 BACKGROUND

MeSH Terms

Interventions

Enzyme-Linked Immunosorbent Assay

Intervention Hierarchy (Ancestors)

Immunoenzyme Techniques; Immunoassay; Immunologic Techniques; Investigative Techniques; Immunosorbent Techniques; Immunohistochemistry; Molecular Probe Techniques

Central Study Contacts

Wesam Ashraf Soliman

CONTACT

01095574120; wesamashraf27@gmail.com

Rania Mohamed Bakry

CONTACT

01013341395; Rbakry.md@aun.edu.eg

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Resident doctor

Study Record Dates

• First Submitted: July 27, 2024
• First Posted: July 31, 2024
• Study Start: January 1, 2025
• Primary Completion (Estimated): April 30, 2028
• Study Completion (Estimated): October 30, 2029
• Last Updated: July 31, 2024

Record last verified: 2024-07