The Effects of Mannose Supplementation on Glycosylation and Protein Levels in Human Sera

NCT06507735 | Completed

• 2 other identifiers: STU-2024-0127Velos Study Number
• Study Type: interventional
• Target: 6
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of our study is to investigate the effects of supplemental mannose on sera of humans who are healthy. We will perform proteomics studies to elucidate changes to glycan structure and protein level changes. We hypothesize that we may find that mannose supplies more properly glycosylated protein substrate and helps expedite protein synthesis, that excess mannose leads to alternative glycan structures which confer improved stability, or that there are new sites that become glycosylated after mannose supplementation.

Conditions

Physiological Stress

Outcome Measures

Primary Outcomes (2)

• Changes to glycan structure

  Glycopeptide mass spectrometry will be applied to the major glycoproteins present in serum.

  ~1 month

• Change in glycoprotein levels

  Standard laboratory methods such as ELISA in either research or clinical laboratory settings (Roche Cobas or similar platform).

  ~1 month

Study Arms (1)

Mannose supplementation

EXPERIMENTAL

Mannose supplementation will be given and subjects will be studied at baseline and after treatment.

Dietary Supplement: Mannose

Interventions

Mannose DIETARY_SUPPLEMENT

Mannose is a sugar monomer that is important in the glycosylation of many proteins. The concentration of mannose in mammalian plasma is \~50-100 μM, which is primarily derived from N-glycan processing. Taking dietary mannose supplements can raise the levels of plasma mannose by 3- to 5-fold. There are currently no known adverse effects of mannose supplementation in humans.

Mannose supplementation

Eligibility Criteria

• Age: 18 Years - 65 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• At least 18 years of age
• No known medical conditions
• Taking no medications or nutritional supplements
• Agree to the study and provide informed consent

You may not qualify if:

• Less than 18 years of age
• Known medical diagnosis, including pregnancy
• Presently taking medications and/or nutritional supplements
• Do not provide consent

Sponsors & Collaborators

• University of Texas Southwestern Medical Center: lead

Study Sites (1)

University of Texas Southwestern Medical Center

Dallas, Texas, 75390, United States

Location

MeSH Terms

Interventions

Mannose

Intervention Hierarchy (Ancestors)

Hexoses; Monosaccharides; Sugars; Carbohydrates

Study Officials

• Jeffrey SoRelle, M.D.

  University of Texas Southwestern Medical Center

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: July 11, 2024
• First Posted: July 18, 2024
• Study Start: October 29, 2024
• Primary Completion: November 6, 2024
• Study Completion: November 6, 2024
• Last Updated: February 27, 2025

Record last verified: 2025-02

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)