Multiplex Respiratory PCR Results in Diabetic Pneumonia Cases

NCT06459713 | Completed

• 1 other identifier: HisarOD
• Study Type: observational
• Target: 151
• Locations: 1 country
• Sites: 1

Brief Summary

Multiplex respiratory polymerase chain reaction (PCR) test has gained wide acceptance as an alternative to conventional culture methods for the detection of the causative agent of community-acquired pneumonia (CAP). The aim of this study is to evaluate the positivity and to determine the causative agent of CAP using multiplex respiratory PCR test in patients with diabetes mellitus.

Conditions

Multiplex Respiratory PCR Results in Diabetic Pneumonia Cases

Keywords

pneumonia, multiplex respiratory PCR test

Outcome Measures

Primary Outcomes (1)

• Diabetic patients are at greater risk for developing CAP. Multiplex respiratory PCR test is an important method for identifying the causative agent of CAP rapidly.

  September 2021 and June 2023

Study Arms (2)

Multiplex respiratory PCR test results of the groups

This single center retrospective observational study was conducted in Hisar Hospital Intercontinental between September 2021 and June 2023

Diagnostic Test: multiplex respiratory polymerase chain reaction test

we evaluated the importance of respiratory PCR in CAP

Subjects who had an infection other than pneumonia, and had a diagnosis of malignancy, transferred to intensive care unit and pregnant women were excluded.

Diagnostic Test: multiplex respiratory polymerase chain reaction test

Interventions

multiplex respiratory polymerase chain reaction test DIAGNOSTIC_TEST

According to our hospital policy, nasopharyngeal swab samples are collected by the clinicians and transported to the Clinical Microbiology Laboratory of the hospital in 10 minutes. The samples are analyzed in 2 hours and the samples were measured by Thermo Fisher / Applied Biosystems Quant Studio-5 for reverse transcription-polymerase chain reaction (RT-PCR) testing to detect multiplex respiratory agents

Multiplex respiratory PCR test results of the groups; we evaluated the importance of respiratory PCR in CAP

Eligibility Criteria

• Age: 18 Years - 90 Years
• Sex: all
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

After the exclusion criteria applied, remaining 151 patients were enrolled in the study. Of the 151 subjects, 42 (27.8%) who were followed up with the confirmed diagnosis of type 2 diabetes mellitus constituted group 1 and 109 (72.2%) ones who did not have a diagnosis of type 2 diabetes mellitus named as group 2. -

You may qualify if:

• In this study, a population of 190 people over the age of 18 were searched in terms of suitability for this work , who diagnosed with CAP after admission to the Department of Chest Diseases by using a multiplex respiratory PCR test.

You may not qualify if:

• Subjects who had an infection other than pneumonia, and had a diagnosis of malignancy, transferred to intensive care unit and pregnant women were excluded.

Sponsors & Collaborators

• Hisar Intercontinental Hospital: lead

Study Sites (1)

Hisar Intercontinental Hospital

Istanbul, 34768, Turkey (Türkiye)

Location

Biospecimen

Retention: SAMPLES WITH DNA

nasopharyngeal swab samples

MeSH Terms

Conditions

Pneumonia

Condition Hierarchy (Ancestors)

Respiratory Tract Infections; Infections; Lung Diseases; Respiratory Tract Diseases

Study Officials

• Yakup Tomak

  Hisar Intercontinental Hospital

  STUDY DIRECTOR

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Clinical Biochemistry

Study Record Dates

• First Submitted: May 31, 2024
• First Posted: June 14, 2024
• Study Start: April 1, 2024
• Primary Completion: May 15, 2024
• Study Completion: May 30, 2024
• Last Updated: June 14, 2024

Record last verified: 2024-05

Locations

TU (1)