NCT06425081

Brief Summary

The goal of this clinical trial is to learn if the probiotic strain Limosilactobacillus reuteri AMBV339 or colon-delivered riboflavin or their combination can change the gut and vaginal microbiome and metabolomics. It will also learn about the safety of the investigational product. The main questions it aims to answer are: Do the probiotic strain Limosilactobacillus reuteri AMBV339 or colon-delivered riboflavin or their combination modify gut microbiome? Do the probiotic strain Limosilactobacillus reuteri AMBV339 or colon-delivered riboflavin or their combination modify gut microbiome? Researchers will compare the probiotic strain Limosilactobacillus reuteri AMBV339 or colon-delivered riboflavin or their combination to a placebo (a look-alike substance that contains no drug) to see if they can change gut and vaginal microbiome. Intervention period is 28 days.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
185

participants targeted

Target at P75+ for not_applicable

Timeline
Completed

Started Sep 2024

Shorter than P25 for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

May 8, 2024

Completed
14 days until next milestone

First Posted

Study publicly available on registry

May 22, 2024

Completed
4 months until next milestone

Study Start

First participant enrolled

September 9, 2024

Completed
5 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 30, 2025

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

January 30, 2025

Completed
Last Updated

May 9, 2025

Status Verified

September 1, 2024

Enrollment Period

5 months

First QC Date

May 8, 2024

Last Update Submit

May 6, 2025

Conditions

Keywords

ProbioticvitaminRiboflavinVitamin B2lactobacillusmicrobiomegut microbiomevaginal microbiomewomen's healthgut health

Outcome Measures

Primary Outcomes (1)

  • Microbiome change in GUT

    1\. To investigate if daily supplementation (for 28 days) with either Humiome B2 or Limosilactobacillus reuteri AMBV339 or their combination modulate the microbiome composition (relative abundance of different taxa) in the gastrointestinal tract (GIT). Comparisons will be made between the active groups and with placebo.

    day 0 - day 28

Secondary Outcomes (15)

  • Microbiome change in vagina

    day 0 - day 28

  • Gut microbiome diversity

    day 0 - day 28

  • Vaginal microbiome diversity

    day 0 - day 28

  • Keystone bacterial taxa in gut

    day 0 - day 28

  • Keystone bacterial taxa in vagina

    day 0 - day 28

  • +10 more secondary outcomes

Other Outcomes (9)

  • Excreted levels of riboflavin (in urine)

    day 0 - day 28

  • Immune markers

    day 0 - day 28

  • General GIT health

    day 0 - day 28

  • +6 more other outcomes

Study Arms (4)

L. reuteri AMBV339

ACTIVE COMPARATOR

Probiotic strain, 1B AFU (per day for 28 days in capsule format)

Dietary Supplement: Limosilactobacillus reuteri AMBV339

Humiome B2

ACTIVE COMPARATOR

73 mg colon delivered riboflavin containing 10 mg pure riboflavin (per day for 28 days in capsule format)

Dietary Supplement: Humiome B2

Combination arm

ACTIVE COMPARATOR

Probiotic strain, 1B AFU + 73 mg colon delivered riboflavin containing 10 mg pure riboflavin (per day for 28 days in capsule format)

Dietary Supplement: Limosilactobacillus reuteri AMBV339 + Humiome B2

Placebo

PLACEBO COMPARATOR

Microcrystalline cellulose (per day for 28 days in capsule format)

Dietary Supplement: Placebo

Interventions

Limosilactobacillus reuteri AMBV339 is a probiotic strain

L. reuteri AMBV339
Humiome B2DIETARY_SUPPLEMENT

Colon-delivered Riboflavin

Humiome B2

Combination of supplements used in arm 1 and 2

Combination arm
PlaceboDIETARY_SUPPLEMENT

Microcrystalline cellulose

Placebo

Eligibility Criteria

Age18 Years - 45 Years
Sexfemale
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Women of reproductive age (appr. 18-45 years old)
  • Women of self-reported good general health
  • Living in Flanders and speaking Dutch
  • Using a combination contraceptive pill (without stop week) during the study and preferably at least three months before the study OR having a hormonal intrauterine device during the study and preferably at least three months before the study
  • Subjects willing and able to give written informed consent and to understand, to participate and to comply with the clinical study requirements.

You may not qualify if:

  • Current pregnancy or breastfeeding
  • Antibiotic/antimycotic use during the last three months before the study
  • Use of group B vitamin supplements or vitamin C during the study (record vitamin use via questionnaires)
  • Ketogenic diet during the study and during the last two weeks before the study
  • Oral and vaginal probiotic, prebiotic, and postbiotic and synbiotic supplementation during the study or recent use during the last two weeks before the study
  • Vaginal douching during the study
  • Presence of general infection
  • Having a reproductive disorder or current vaginal infection or vaginal symptoms (VVC, BV, AV, etc.)
  • Having a gastro-intestinal disorder or current GIT infections or gastrointestinal disorders (Crohn, IBS, IBD, etc.)
  • Participation in another clinical trial

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Lab of Applied Microbiology and Biotechnology, University of Antwerp

Antwerp, Antwerp, 2020, Belgium

Location

MeSH Terms

Conditions

Communicable Diseases

Condition Hierarchy (Ancestors)

InfectionsDisease AttributesPathologic ProcessesPathological Conditions, Signs and Symptoms

Study Officials

  • Veronique Verhoeven, Dr

    University of Antwerp Department of Family Medicine and Population Health

    PRINCIPAL INVESTIGATOR
  • Sarah Lebeer, Dr

    University of Antwerp Department of Bioscience Engineering

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
QUADRUPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
Masking Details
The study will double blinded
Purpose
SCREENING
Intervention Model
PARALLEL
Model Details: Double-blinded, randomized and placebo-controlled clinical trial
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

May 8, 2024

First Posted

May 22, 2024

Study Start

September 9, 2024

Primary Completion

January 30, 2025

Study Completion

January 30, 2025

Last Updated

May 9, 2025

Record last verified: 2024-09

Locations