Metabolic Availability in Older and Younger Men

NCT06332651 | Completed

• 1 other identifier: NL84684.091.23
• Study Type: interventional
• Target: 28
• Locations: 1 country
• Sites: 1

Brief Summary

The main goal of this study is to determine the metabolic availability of methionine in black beans, lysine in sorghum and lysine in milk using the indicator amino acid oxidation method both in older and in younger men.

Conditions

Ageing

Outcome Measures

Primary Outcomes (1)

• Metabolic availability of methionine in black beans and lysine in milk and sorghum measured with the indicator amino acid oxidation method.

  Indicator amino acid oxidation method is used to measure metabolic availability of the limiting amino acid by measuring the oxidation of the 13C-labelled indicator amino acid in expired breath as 13CO2 when giving different levels of the limiting amino acid. Methionine metabolic availability will be assessed in black beans and lysine metabolic availability in milk and sorghum.

  1 measurement to be calculated from repeated measurements on several test days separated by 1 week

Study Arms (4)

Methionine in black beans in older men

EXPERIMENTAL

This group of 7 participants will have 3 test days where they consume black beans with 3 different levels of methionine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of methionine.

Other: Black beans

Methionine in black beans in younger men

EXPERIMENTAL

This group of 7 participants will have 3 test days where they consume black beans with 3 different levels of methionine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of methionine.

Other: Black beans

Lysine in milk or sorghum in older men

EXPERIMENTAL

This group of 7 participants will have 3 test days where they consume milk with 3 different levels of lysine, they will have 3 test days where they consume sorghum with 3 different levels of lysine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of lysine.

Other: Milk; Other: Sorghum

Lysine milk or sorghum in younger men

EXPERIMENTAL

This group of 7 participants will have 3 test days where they consume milk with 3 different levels of lysine, they will have 3 test days where they consume sorghum with 3 different levels of lysine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of lysine.

Other: Milk; Other: Sorghum

Interventions

Black beans OTHER

Black beans cooked and mashed and amino acid mixture as liquid drink to make methionine the limiting amino acid in the food consumed

Also known as: Nutrition

Methionine in black beans in older men; Methionine in black beans in younger men

Milk OTHER

Skimmed milk as liquid drink and amino acid mixture as liquid drink to make lysine the limiting amino acid in the food consumed

Also known as: Nutrition

Lysine in milk or sorghum in older men; Lysine milk or sorghum in younger men

Sorghum OTHER

Sorghum cooked and mashed and amino acid mixture as liquid drink to make lysine the limiting amino acid in the food consumed

Also known as: Nutrition

Lysine in milk or sorghum in older men; Lysine milk or sorghum in younger men

Eligibility Criteria

• Age: 20 Years - 80 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Gender: Male Age: 20-35 years or 65-80 years Body Mass Index (BMI): 18.5 - 30.0 kg/m2 Healthy as assessed with a questionnaire Regular and normal eating habits as assessed with a Food Frequency Questionnaire (FFQ) Willing to comply with study procedures and use of data Having given written informed consent

You may not qualify if:

• Chronic disease, for example:
• Diabetes mellitus / being treated for high blood glucose
• Severe cardiovascular disease (exception: hypertension)
• Hepatic disease (e.g. hepatitis)
• Renal disease
• Cancer
• Bowel disease (e.g. inflammatory bowel disease, ulcers, bleeding)
• Pancreatitis
• History of medical or surgical events that may affect GI function, and the study outcomes or disease risk for participants, for example:
• Bariatric surgery
• Gastrointestinal tract surgery
• Digestive tract disorder
• Chewing problems
• History of deep venous thrombosis without anti-coagulation medication
• Medicine use that interferes with, protein metabolism, GI function and the study outcomes, for example:

Sponsors & Collaborators

• Wageningen University: lead

Study Sites (1)

Wageningen University & Research

Wageningen, Gelderland, 6708PB, Netherlands

Location

MeSH Terms

Interventions

Nutritional Status; Milk

Intervention Hierarchy (Ancestors)

Nutritional Physiological Phenomena; Diet, Food, and Nutrition; Physiological Phenomena; Health Status; Demography; Population Characteristics; Beverages; Dairy Products; Food; Food and Beverages

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: PREVENTION
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Model Details: It is a parallel intervention study. The intervention does not involve a drug or biologic product, however does involve an adaptation diet and a diet with measured amino acids dissolved in water, during the test day. We investigate the amino acid metabolic availability using the IAAO method.

Study Record Dates

• First Submitted: December 19, 2023
• First Posted: March 27, 2024
• Study Start: February 19, 2024
• Primary Completion: June 30, 2025
• Study Completion: July 15, 2025
• Last Updated: May 7, 2026

Record last verified: 2025-07

Locations

NL (1)