NCT06268626

Brief Summary

The study goal is to investigate a non-invasive approach to predict endometrial cancer (EC) risk, better understand disease progression and identify opportunities for intervention. This two-part case-cohort prospective study will recruit patients whose abnormal uterine bleeding is being evaluated via endometrial biopsy. Participants will complete an online health questionnaire, and a subset will be invited to self-collect vaginal samples for sequencing. Selected sequenced participants will be invited for longitudinal monitoring (questionnaires, wearable fitness tracker) and an additional vaginal self-collection to identify persistent genetic mutations or microbiome alterations 6-8 months later.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
1,000

participants targeted

Target at P75+ for all trials

Timeline
5mo left

Started Oct 2024

Typical duration for all trials

Geographic Reach
1 country

1 active site

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress82%
Oct 2024Jan 2027

First Submitted

Initial submission to the registry

January 30, 2024

Completed
21 days until next milestone

First Posted

Study publicly available on registry

February 20, 2024

Completed
8 months until next milestone

Study Start

First participant enrolled

October 10, 2024

Completed
2.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 1, 2027

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

January 1, 2027

Last Updated

April 16, 2026

Status Verified

April 1, 2026

Enrollment Period

2.2 years

First QC Date

January 30, 2024

Last Update Submit

April 13, 2026

Conditions

Keywords

ScreeningPreventionWomen's HealthReproductive healthAbnormal Uterine Bleeding

Outcome Measures

Primary Outcomes (2)

  • Diagnostic Performance of cfDNA Mutation Detection for Endometrial Pathology

    Cell-free DNA (cfDNA) extracted from vaginal swabs will be sequenced to identify endometrial cancer-associated mutations. Diagnostic performance will be evaluated by calculating sensitivity, specificity, accuracy, positive predictive value, and negative predictive value for detecting endometrial pathology, using biopsy-confirmed pathology as the reference standard.

    Through study completion, anticipated 1-2 years

  • Association Between Vaginal Microbiome Profile and Endometrial Pathology

    Vaginal microbiome DNA extracted from swabs will be sequenced and processed into operational taxonomic units (OTUs) using an in-house bioinformatics pipeline. OTUs will be compared across biopsy-confirmed pathology groups and evaluated against previously published microbiome signatures predictive of endometrial cancer.

    Through study completion, anticipated 1-2 years

Study Arms (3)

EIN/EC BIOPSY RESULT

Vaginal samples sequenced. Participation ends here.

EH BIOPSY RESULT

Vaginal samples sequenced. Subset invited to move on to longitudinal monitoring and samples sequenced for 6 additional months.

NEGATIVE BIOPSY RESULT

Control for natural and spontaneous changes in vaginal samples sequenced. Random subset selected to move on to longitudinal monitoring and samples sequenced for 6 additional months.

Eligibility Criteria

Age40 Years+
Sexfemale
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

The study investigators will have 10-15 study gynecologists across British Columbia, Canada, who will be recruiting study participants from patients who are referred to them for an endometrial biopsy due to abnormal uterine bleeding. The investigators aim to recruit \~1000 participants in Study Part A in order to achieve their target sample size of n=50 EIN/EC and n=150 EH. Sequenced participants will be invited to participate in Study Part B/ Longitudinal monitoring and provide additional samples.

You may qualify if:

  • Study Part A:
  • years and older
  • Experiencing unexplained abnormal uterine bleeding (i.e., not from IUD, etc.)
  • Have an intact uterus
  • Referred for an endometrial biopsy
  • Study Part B/Longitudinal monitoring:
  • Those selected for sequencing (from Part A) and who retained their uterus.

You may not qualify if:

  • Study Part A:
  • Endometrial sampling, pelvic radiation, or vaginal infection (vaginosis, yeast) in the past 3 months
  • Started hormone therapy (HRT, birth control, IUD) in the past year (with the exception of tamoxifen)
  • Intercourse, vaginal product use, or douching in the past 48 hours
  • Study Part B/Longitudinal monitoring:
  • Same as Study Part A
  • EC or EIN, or anyone who is recommended a hysterectomy

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

VGH Research Pavilion

Vancouver, British Columbia, V5Z 1M9, Canada

RECRUITING

Related Publications (1)

  • Pfeiffer RM, Park Y, Kreimer AR, Lacey JV Jr, Pee D, Greenlee RT, Buys SS, Hollenbeck A, Rosner B, Gail MH, Hartge P. Risk prediction for breast, endometrial, and ovarian cancer in white women aged 50 y or older: derivation and validation from population-based cohort studies. PLoS Med. 2013;10(7):e1001492. doi: 10.1371/journal.pmed.1001492. Epub 2013 Jul 30.

    PMID: 23935463BACKGROUND

Biospecimen

Retention: SAMPLES WITH DNA

Vaginal DNA Vaginal microbiome Vaginal pH

MeSH Terms

Conditions

Endometrial NeoplasmsMetrorrhagia

Condition Hierarchy (Ancestors)

Uterine NeoplasmsGenital Neoplasms, FemaleUrogenital NeoplasmsNeoplasms by SiteNeoplasmsUterine DiseasesGenital Diseases, FemaleFemale Urogenital DiseasesFemale Urogenital Diseases and Pregnancy ComplicationsUrogenital DiseasesGenital DiseasesUterine HemorrhageHemorrhagePathologic ProcessesPathological Conditions, Signs and Symptoms

Study Officials

  • Aline Talhouk, PhD

    University of British Columbia

    PRINCIPAL INVESTIGATOR
  • Anna Tinker, MD

    University of British Columbia

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Aline Talhouk, PhD

CONTACT

Jennifer Ellis-White

CONTACT

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Principal Investigator

Study Record Dates

First Submitted

January 30, 2024

First Posted

February 20, 2024

Study Start

October 10, 2024

Primary Completion (Estimated)

January 1, 2027

Study Completion (Estimated)

January 1, 2027

Last Updated

April 16, 2026

Record last verified: 2026-04

Data Sharing

IPD Sharing
Will not share

Locations