NCT06219135

Brief Summary

Sepsis is a disruption of homeostasis in the human body in response to bloodstream infection and is associated with a high risk of mortality. Worldwide, sepsis is affecting approximately 30 million people and resulting in six million deaths. Blood culture is a specific blood sample used to identifying microbial agent (bacterium or yeast) and determine the sensitivity of these microorganisms to antibiotics and antifungals. Any delay in identifying the microorganism and/or determining the AST (antibiotic susceptibility testing) has a direct impact on the administration of appropriate antibiotic treatment and, consequently, on mortality of the patient. The faster the diagnosis, the faster the antibiotic treatment will be adapted, the higher the survival rate/probability of patients, and the lower the ecological impact. In routine, clinical microbiology laboratories currently use 2 automatized techniques: MALDI-TOF MS® for microorganisms identification and VITEK2® method for AST determination. Based on a proteomic approach, the IDBIORIV method is a rapid method (90 minutes) in comparison of current methods (24/48 hours) able to identifying a large panel of 113 pathogens and determine the antibiotic resistance profile of 49 species for 4 classes of antibiotics (Beta-lactams, Aminosides, Glycopeptides, Colistin). The main objective of this study is to evaluate the performance of the IDBIORIV method in pathogen identification and antibiotic susceptibility testing in comparison with current methods of analysis of positive blood cultures used at the microbiology laboratory of the Hospices Civils de Lyon, in a real clinical situation, over a 2-year period.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
1,372

participants targeted

Target at P75+ for all trials

Timeline
6mo left

Started Mar 2024

Typical duration for all trials

Geographic Reach
1 country

23 active sites

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress84%
Mar 2024Nov 2026

First Submitted

Initial submission to the registry

December 6, 2023

Completed
2 months until next milestone

First Posted

Study publicly available on registry

January 23, 2024

Completed
2 months until next milestone

Study Start

First participant enrolled

March 21, 2024

Completed
2.7 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 21, 2026

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

November 21, 2026

Last Updated

June 10, 2026

Status Verified

June 1, 2026

Enrollment Period

2.7 years

First QC Date

December 6, 2023

Last Update Submit

June 8, 2026

Conditions

Keywords

Sepsisbacteriamicrobiology diagnosis methodidentificationresistanceantibiotic susceptibility testingvirulence

Outcome Measures

Primary Outcomes (2)

  • The IDBIORIV method will be compared with the current routine methods present in the Hospices Civils de Lyon. The sensitivity and specificity of the IDBIORIV method will be evaluated using the Identification (ID) results of a positive blood culture for a

    Reference technique used = Vitek MS ® (MALDI-ToF) bioMérieux.

    24 Hours to 48 Hours after inclusion

  • The IDBIORIV method will be compared with the current routine methods present in the Hospices Civils de Lyon. The sensitivity and specificity of the IDBIORIV method will be evaluated using AST results. Reference technique = Vitek 2® bioMérieux

    The performance of the IDBIORIV method for in vitro diagnosis of antibiotic-resistant/susceptible status will be evaluated for each "species-antibiotic" pair and "species-antibiotic group" of interest. Sensitivity will be calculated as the proportion of pairs diagnosed as resistant using the IDBIORIV method among pairs diagnosed as resistant using AST. Specificity will be calculated as the proportion of pairs diagnosed as sensitive with the IDBIORIV method among pairs diagnosed as sensitive with AST.

    24 Hours to 48 Hours after inclusion

Study Arms (3)

Contamination

Patients with a positive blood culture due to contamination microorganism

Other: no intervention - Contamination group

Bacteraemia

This group includes all adults and children with a positive blood culture due to pathogen microorganism

Biological: blood sample - Bacteraemia group

Emergency

Emergency Room Patient's with a positive blood culture due to pathogen and/or contamination microorganism

Other: no intervention - Emergency group

Interventions

there is no intervention for this group of patients

Contamination

for adult patients, in optional, blood sample will be taken. 27,5 ml of blood at H24-48 after inclusion and 5 ml of blood at day 30

Bacteraemia

there is no intervention for this group of patients

Emergency

Eligibility Criteria

Age1 Day+
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

This study will focus on adult and pediatric patients hospitalized in 23 clinical departments of the Hospices Civils de Lyon and presenting a first positive blood culture detected on the microbiology laboratory of the Institut des Agents Infectieux.

You may qualify if:

  • Adult or child patient
  • Hospitalized in one of the departments registered with Hospices Civils de Lyon
  • Showing signs of infection
  • Confirmed by a 1st positive blood culture
  • Patient or close relative (trustworthy person or family member) or holder of parental authority who has given his or her non-objection after receiving the information note

You may not qualify if:

  • Patients under court protection
  • Patients under guardianship or curatorship

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (23)

Femme Mère enfant Hospital

Bron, 69394, France

RECRUITING

Femme Mère Enfant Hospital

Bron, 69394, France

RECRUITING

Femme Mère enfant Hospital

Bron, 69394, France

NOT YET RECRUITING

Louis Pradel Hospital

Bron, 69394, France

RECRUITING

Louis Pradel Hospital

Bron, 69394, France

RECRUITING

Louis Pradel Hospital

Bron, 69394, France

RECRUITING

Pierre Wertheimer Hospital

Bron, 69394, France

RECRUITING

Edouard Herriot Hospital

Lyon, 69003, France

RECRUITING

Edouard Herriot Hospital

Lyon, 69003, France

RECRUITING

Edouard Herriot Hospital

Lyon, 69003, France

NOT YET RECRUITING

Edouard Herriot Hospital

Lyon, 69003, France

NOT YET RECRUITING

Croix Rousse Hospital

Lyon, 69004, France

RECRUITING

Croix Rousse Hospital

Lyon, 69004, France

NOT YET RECRUITING

Croix Rousse Hospital

Lyon, 69004, France

RECRUITING

Croix Rousse Hospital

Lyon, 69004, France

RECRUITING

Croix Rousse Hospital

Lyon, 69004, France

RECRUITING

Croix Rousse Hospital

Lyon, 69004, France

RECRUITING

Croix Rousse Hospital

Lyon, 69004, France

NOT YET RECRUITING

Lyon Sud Hospital

Pierre-Bénite, 69495, France

RECRUITING

Lyon Sud Hospital

Pierre-Bénite, 69495, France

RECRUITING

Lyon Sud Hospital

Pierre-Bénite, 69495, France

NOT YET RECRUITING

Lyon Sud Hospital

Pierre-Bénite, France

NOT YET RECRUITING

Charpennes Hospital

Villeurbanne, 69100, France

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

For each participant, a strain of the detected pathogen will be stored at the Institut des Agents Infectieux of the Centre de Biologie and Pathologie Nord of the Hôpital de la Croix Rousse 69004 Lyon. In option, for adult patients with Bacteraemia (bacteraemia group only), a blood sample will be drawn at visit H24-48 and J30. Analysis will be done at Laboratoire Commun de Recherche Hospices Civils de Lyon-bioMérieux, 69310 Pierre Bénite

MeSH Terms

Conditions

Sepsis

Condition Hierarchy (Ancestors)

InfectionsSystemic Inflammatory Response SyndromeInflammationPathologic ProcessesPathological Conditions, Signs and Symptoms

Study Officials

  • François VANDENESCH, Pr

    Hospices Civils LYON

    STUDY DIRECTOR

Central Study Contacts

François VANDENESCH, Pr

CONTACT

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

December 6, 2023

First Posted

January 23, 2024

Study Start

March 21, 2024

Primary Completion (Estimated)

November 21, 2026

Study Completion (Estimated)

November 21, 2026

Last Updated

June 10, 2026

Record last verified: 2026-06

Locations