Evaluation of Mitochondrial Enzyme Activity in Cesarean Section Patients

NCT06206928 | Unknown

• 1 other identifier: HRÜ/22.15.11
• Study Type: observational
• Target: 50
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this observational study is to learn about in effects of trace element metabolism, tricarboxylic acid cycle enzyme activities and thiol/disulfide balance in patients who underwent cesarean section under spinal anesthesia. The main question\[s\] it aims to answer are: • What is the effect of trace element metabolism, tricarboxylic acid cycle enzyme activities and thiol/disulfide balance in patients undergoing cesarean section under regional anesthesia? A 10 ml blood sample will be taken from the participants to analyze the biochemical parameters mentioned before and after the operation. Pre- and post-operative values will be compared.

Conditions

Cesarean Section; Spinal Anesthesia; Thiol Groups

Keywords

cesarean section; spinal anesthesia; mitochondrial enzyme changes; thiol disulfide balance

Outcome Measures

Primary Outcomes (1)

• Biochemical parameter measurements that we mentioned in the description

  Serum zinc concentrations were measured with commercially available Rel Assay zinc measurement kit (Gaziantep, Turkey) using a fully automatic photometric method (Abbott ARCHITECT c8000 clinical chemistry analyzer). Serum copper concentrations were measured with commercially available Rel Assay copper measurement kit (Gaziantep, Turkey), using a fully automatic photometric method (Abbott ARCHITECT c8000 clinical chemistry analyzer). Serum levels of total and native thiol were measured with a novel method developed by Erel and Neselioglu. In this method, serum dynamic disulfide bonds (-S-S-) is reduced to native thiol groups (-SH HS-) by sodium borohydride (NaBH4) and native thiol levels were measured.

  1 day

Eligibility Criteria

• Age: 18 Years - 40 Years
• Gender Eligibility Details: Since this study was planned in patients who underwent cesarean section under spinal anesthesia
• Healthy Volunteers: No
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

Patients who will undergo planned cesarean section between the ages of 18 and 40

You may qualify if:

• Patients who will undergo planned cesarean section between the ages of 18 and 40
• ASA 1-2 Patients

You may not qualify if:

• Patients who refuse to participate in the study
• Patients over 40 years of age
• ASA 3-4 Patients
• Patients who underwent emergency cesarean section
• Those with known liver and kidney diseases, patients diagnosed with Diabetes Mellitus
• Pregnant women diagnosed with an abnormal baby
• Patients diagnosed with preeclampsia
• Patients with body mass index ≥ 30
• Patients who do not accept spinal anesthesia or have contraindications to the application -

Sponsors & Collaborators

• Sanliurfa Mehmet Akif Inan Education and Research Hospital: lead

Study Sites (1)

Şanlıurfa Training and Research Hospital

Sanliurfa, 63250, Turkey (Türkiye)

Location

Study Officials

• MEHMET TERCAN, Assoc Prof

  University of Health science

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Mehmet Tercan, Assoc Prof

CONTACT

+905323445635; drmtercan@gmail.com

Ahmet Kaya, Assoc Prof

CONTACT

+905327010609; ahmetkayamd@yahoo.com

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: PROSPECTIVE
• Target Duration: 30 Days
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate professor

Study Record Dates

• First Submitted: December 27, 2023
• First Posted: January 16, 2024
• Study Start: January 20, 2024
• Primary Completion: January 20, 2024
• Study Completion: February 1, 2024
• Last Updated: January 16, 2024

Record last verified: 2024-01

Locations

TU (1)