Metabolic Availability of Methionine in Mung Beans

NCT06184984 | Not Yet Recruiting

• 1 other identifier: FIERC-2024-030
• Study Type: interventional
• Target: 6
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this study is to determine the protein quality of Philippine mung beans. The main objective of this study is to determine the metabolic availability of methionine in mung beans that the body can use. We will test the mung beans by studying them after cooking them as sauté. This research is being done in order to bridge the gap between knowledge of protein requirement and the amount of food needed to meet that requirement. Results from this study will be important for recommendations guiding food choices of mung beans as a major protein source in the diet. Each study participant will be part of seven (7) different experimental diets, and randomly assigned to one of the diets every time. Four (4) reference diets would be based on egg protein composition, and three (3) test diets will have protein from cooked mung beans. Each experimental diet will be studied over 3 days: 2 adaptation days and 1 study day. The meals during the 2-day adaptation period would be consumed at home. On the study day, following a 10 to 12-h overnight fast, the study participants will come to the research unit at the Department of Science - Food and Nutrition Research Institute (DOST-FNRI), Gen. Santos Ave., Bicutan, Taguig City, Philippines, for a period of 8 to 9 hours and consume the diets as 9 hourly meals.

Conditions

Healthy Women of Child Bearing Potential

Keywords

Metabolic Availability; Methionine; IAAO; Mung beans

Outcome Measures

Primary Outcomes (1)

• Metabolic availability of methionine in mung beans

  Protein quality of mung beans measured as metabolic availability using the IAAO method

  2 years

Study Arms (1)

Methionine metabolic availability in mung beans

EXPERIMENTAL

Participants will be seen initially for pre-study assessment. They will then be studied for up to 7 times. The participants will be expected to participate in 7 different sets of experiments which will take 2 to 3 months. Each set of experiment consists of a 3 day period. During the first 2 days (Adaptation Days) you will be expected to consume 4 meals per day consisting of a protein liquid drink and protein free-cookies and/or cooked mung bean, which will be provided by the investigators.

Dietary Supplement: Metabolic availability of methionine in mung beans

Interventions

Metabolic availability of methionine in mung beans DIETARY_SUPPLEMENT

Each participant will randomly receive 7 different levels of methionine provided as free amino acids and cooked mung beans.

Methionine metabolic availability in mung beans

Eligibility Criteria

• Age: 19 Years - 40 Years
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Non-pregnant
• Non-lactating
• Stable body weight
• Healthy, with no known clinical condition that would affect protein or AA metabolism, e.g. Diabetes
• Not on any medications that could affect protein or amino acid metabolism e.g. steroids.

You may not qualify if:

• Unwillingness to participate or unable to tolerate the diet
• Recent history of weight loss within the last 3 months or on a weight-reducing diet
• Inability to tolerate study diets (e.g. Allergy to ingredients)

Sponsors & Collaborators

• Food and Nutrition Research Institute, Philippines: lead
• International Atomic Energy Agency: collaborator
• Mahidol University: collaborator

Study Sites (1)

Department of Science and Technology - Food and Nutrition Research Institute

City of Taguig, National Capital Region, 1630, Philippines

Location

Study Officials

• Johanne B Guilaran, Bachelor's

  Department of Science and Technology - Food and Nutrition Research Institute

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Johanne B Guilaran, Bachelor's

CONTACT

(63) 02 8837-8113; yuyanbguilaran@gmail.com

Ma. Lourdes T Cumagun, Bachelor's

CONTACT

(63) 02 8837-8113; maloucumagun@yahoo.com

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER GOV
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: December 14, 2023
• First Posted: December 29, 2023
• Study Start: April 1, 2025
• Primary Completion: December 1, 2025
• Study Completion: December 1, 2025
• Last Updated: February 13, 2025

Record last verified: 2024-10

Data Sharing

• IPD Sharing: Will not share

Locations

PH (1)