Effect of 8-week Supplementation With Black Chokeberry on Recovery in Highly Trained Rowers

NCT06133751 | Completed Phase 4 DMC

• 1 other identifier: SKaczmarczyk_PhD
• Study Type: interventional
• Target: 15
• Locations: 1 country
• Sites: 1

Brief Summary

Purpose: To analyze the effects of 8-week supplementation with anthocyanin compounds contained in black chokeberry (Aronia melanocarpa) on indicators of inflammation and oxidative stress. Hypothesis: Supplementation with anti-inflammatory and antioxidant anthocyanin compounds improves recovery from intense exercise by reducing inflammation and oxidative stress in highly trained rowers The study consisted of a nutritional intervention (supplementation) - with compounds of natural origin - chokeberry extract (capsules) - 18% standardized for anthocyanin content (dose 3 x 200 mg per day) - that is, 107 mg of pure anthocyanins per day, or a placebo product that was made from chokeberry fiber Study plan I term of the study

1. 1.Conduct a body composition analysis, body height, food diary, self reported gastrointestinal scale
2. 2.Collection of blood samples before the exercise test for biochemical determinations.
3. 3.Exercise test of 2000m on a rowing ergometer.
4. 4.Collection of blood immediately after exercise and 1 hours after
5. 5.Collection of blood for biochemical determinations 24 hours after the end of the exercise test
6. 6.Supplementation for 8 weeks 2nd test date Repeat the measurements from the 1st test date.

Conditions

Inflammation;Muscle(S); Oxidative Stress Injury; Permeability; Increased

Outcome Measures

Primary Outcomes (11)

• Level of total antioxidants (TAC)

  ELISA kits (SunRed Biotechnology Company) - Indicators of pro-oxidant-antioxidant balance

  8 weeks

• interleukins: IL-2, IL-6, IL-10

  ELISA kits (SunRed Biotechnology Company) - Immunological indicators

  8 weeks

• lactic acid

  Diaglobal, Berlin, Germany)

  8 weeks

• LBP (lipopolysacharide-binding pro)

  ELISA kits (SunRed Biotechnology Company) - Gut parameter

  8 weeks

• level of lipid peroxidation products (TBARS)

  ELISA kits (SunRed Biotechnology Company) - Indicators of pro-oxidant-antioxidant balance

  8 weeks

• (NO) nitric oxide

  obtained by indirect method Griessa - Indicators of pro-oxidant-antioxidant balance

  8 weeks

• comet assay (DNA damage)

  comet assay BioTechne

  8 weeks

• tumor necrosis factor (TNF α)

  ELISA kits (SunRed Biotechnology Company) - Immunological indicators

  8 weeks

• myoglobin

  ELISA kits (SunRed Biotechnology Company)

  8 weeks

• I-FABP (intestinal fatty acid binding protein)

  ELISA kits (SunRed Biotechnology Company) - Gut parameter

  8 weeks

• Claudin3

  ELISA kits (SunRed Biotechnology Company) - Gut parameter

  8 weeks

Secondary Outcomes (11)

• gastrointestinal symptom scale

  8 weeks

• energy [kcal]

  8 weeks

• protein [g]

  8 weeks

• fat [g]

  8 weeks

• carbohydrate [g]

  8 weeks

Study Arms (2)

Supplemented group

EXPERIMENTAL

Athletes in the supplemented group (n=7) consumed capsules with highly concentrated 18% chokeberry extract. One capsule contained 200 mg. In addition to chokeberry extract, each capsule also contained chokeberry fiber, E460b magnesium salts of stearic acid (anti-caking agent), E551 silicon dioxide (anti-caking agent), hydroxypropyl methylcellulose (capsule shell) and E171 titanium dioxide (shell color). The rowers (both supplemented and control group) took 3 capsules a day (at breakfast, lunch and dinner) for 8 weeks. The athletes sipped each capsule with a glass of water.

Drug: black chokeberry (Aronia melanocarpa)

Placebo group

PLACEBO COMPARATOR

The control group (n=8) consumed capsules that were made from chokeberry fiber. The rowers (both supplemented and control group) took 3 capsules a day (at breakfast, lunch and dinner) for 8 weeks. The athletes sipped each capsule with a glass of water.

Drug: Placebo

Interventions

black chokeberry (Aronia melanocarpa) DRUG

Black chokeberry capsules standardized for anthocyanin compounds

Supplemented group

Placebo DRUG

Chokeberry fiber placebo capsules

Placebo group

Eligibility Criteria

• Age: 18 Years - 24 Years
• Sex: male
• Healthy Volunteers: No
• Age Groups: Adult (18-64)

You may qualify if:

• Training experience a minimum of five years
• Minimum training time per week 240 minute
• Membership in the Youth National Rowing Team of Poland
• Completion of the 2000m ergometer test on two test dates

You may not qualify if:

• Taking other supplements containing anthocyanin compounds during the study period or a month earlier
• Antibiotic therapy
• Health problems

Sponsors & Collaborators

• Poznan University of Physical Education: lead

Study Sites (1)

University of Physical Education in Poznan

Poznan, Poland

Location

Related Publications (1)

• Kaczmarczyk S, Kasperska A, Dziewiecka H, Ostapiuk-Karolczuk J, Cichon-Wozniak J, Basta P, Skarpanska-Stejnborn A. A single 2000-meter exercise test to assess exercise adaptation in elite rowers during the preparatory phase. Front Physiol. 2025 Aug 12;16:1544637. doi: 10.3389/fphys.2025.1544637. eCollection 2025.

  PMID: 40873756 DERIVED

MeSH Terms

Conditions

Myositis, Inclusion Body

Condition Hierarchy (Ancestors)

Myositis; Muscular Diseases; Musculoskeletal Diseases; Neuromuscular Diseases; Nervous System Diseases

Study Officials

• Anna Skarpańska-Stejnborn, Professor

  Poznan University of Physical Education

  STUDY DIRECTOR

Study Design

• Study Type: interventional
• Phase: phase 4
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Masking Details: Youth National Rowing Team of Poland
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: Participants randomly divided into one of two groups. The supplemented group (n=7) receives chokeberry capsules, while the control group (n=8) receives a placebo product.

Study Record Dates

• First Submitted: October 27, 2023
• First Posted: November 15, 2023
• Study Start: April 1, 2023
• Primary Completion: June 10, 2023
• Study Completion: June 10, 2023
• Last Updated: April 26, 2024

Record last verified: 2023-10

Data Sharing

• IPD Sharing: Will not share

Locations

PL (1)