NCT06131424

Brief Summary

This noninterventional, multicenter,retrospective study has been proposed to estimate the prevalence, clinicopathological characteristics,treatment patterns and clinical outcomes of human epidermal growth factor receptor 2 -(HER2)low locally-advanced or metastatic breast cancer(mBC) by accurate rescoring of archived IHC-stained formalin-fixed paraffin-embedded (FFPE) slides for HER2 in patients previously identified as HER2-negative from emerging markets of international regions (non-US and non-European region) with largely unknown prevalence estimates of HER2 low mBCs. Patients with a confirmed diagnosis of HER2-negative, locally-advanced or mBC regardless of Hormone receptor (HR)status between 01 January 2019 and 31 December 2022 who progressed on any systematic anticancer therapy (eg, ET, chemotherapy, CDK4/6 inhibitor, targeted therapies other than anti-HER2, or immunotherapy) in advanced disease with availability of atleast 12 months of follow-up data (from the index date) in the medical records at the participating site, unless patient died within the first 12 months of diagnosis of locally-advanced or mBC will be enrolled in the study. The HR positive patients will be considered eligible for the study if they have received ET as adjuvant therapy in the early BC setting and progressed within 24 months. This scenario will be considered as progression on systematic treatment in the advanced or metastatic setting.

Trial Health

98
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
1,151

participants targeted

Target at P75+ for all trials

Timeline
Completed

Started Apr 2024

Geographic Reach
13 countries

31 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

November 9, 2023

Completed
5 days until next milestone

First Posted

Study publicly available on registry

November 14, 2023

Completed
5 months until next milestone

Study Start

First participant enrolled

April 23, 2024

Completed
1.1 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

May 19, 2025

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

May 19, 2025

Completed
Last Updated

September 18, 2025

Status Verified

September 1, 2025

Enrollment Period

1.1 years

First QC Date

November 9, 2023

Last Update Submit

September 17, 2025

Conditions

Keywords

HER2 Low, Metastatic breast cancerHR:Hormone Receptor, IHC:mmunohistochemistryISH: in situ hybridizationAnticancer therapyPrognostic and Predictive BiomarkerFormalin-fixed, paraffin-embedded (FFPE)endocrine therapy(ET)Subsets

Outcome Measures

Primary Outcomes (2)

  • Proportion of patients with IHC 1+ or IHC 2+/ISH- HER2 expression.

    1\. Proportion of patients with IHC 1+ or IHC 2+/ISH- HER2 expression among previously identified HER2-negative locally-advanced or mBC patients based on rescoring of archived IHC-stained FFPE slides.

    6 months from the Last subject In

  • Proportion of patients with HER2 ultra-low (IHC 0 (pattern 0+) with membrane staining ie, incomplete and faint/barely perceptible in ≤10% tumor cells) HER2 expression and IHC null (IHC 0 ie, absent membrane staining),

    Proportion of patients with HER2 ultra-low (IHC 0 (pattern 0+) with membrane staining ie, incomplete and faint/barely perceptible in ≤10% tumor cells) HER2 expression and IHC null (IHC 0 ie, absent membrane staining), expanded biomarker association with HER2-low patient characteristics, change in HER2 and HR expressions between diagnosis and treatment, concordance of HER2 IHC scores between manual rescoring and digital scoring.

    6 months from the Last subject In

Secondary Outcomes (4)

  • Sociodemographic (age, gender, smoking status, country, ethnicity, site type [community hospital, academic hospital, biobank, research institute], family history of BC) and clinical pathological characteristics

    6 months from the Last subject In

  • Proportions of patients in the overall study population and each study subset receiving the following treatments for early-stage BC (if available), locally-advanced or mBC

    6 months from the Last subject In

  • Clinical outcomes

    6 months from the Last subject In

  • Concordance between rescoring IHC status as HER2-low by central laboratory and/or local laboratory

    6 months fromthe last subject In

Other Outcomes (1)

  • Summary of the following biomarkers using available historical next generation sequencing (NGS) or IHC results

    6 months from the Last subject In

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodProbability Sample
Study Population

Eligible patients from sites will be selected in a consecutive manner ie, patients with dates closest to but on or after 01 January 2019 will be selected first.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (31)

Research Site

Rosario, Argentina

Location

Research Site

São Paulo, Brazil

Location

Research Site

Santo Domingo, Dominican Republic

Location

Research Site

Hong Kong, Hong Kong

Location

Research Site

Bengaluru, Karnataka, 560017, India

Location

Research Site

Kochi, Kerala, 682041, India

Location

Research Site

Mumbai, Maharashtra, 400012, India

Location

Research Site

Bhubaneshwar, Odisha, 751003, India

Location

Research Site

Mohali, Punjab, 160055, India

Location

Research Site

Hyderabad, Telangana, 500034, India

Location

Research Site

Varanasi, Uttar Pradesh, 221005, India

Location

Research Site

Delhi, 110005, India

Location

Research Site

Delhi, 110029, India

Location

Research Site

Delhi, 110085, India

Location

Research Site

Jakarta, Indonesia

Location

Research Site

Kaula Lumpur, 47500, Malaysia

Location

Research Site

Subang Jaya, 47500, Malaysia

Location

Research Site

Subang Jaya, 59100, Malaysia

Location

Research Site

Mexico City, Mexico

Location

Research Site

Panama City, Panama

Location

Research Site

Quezon City, 1112, Philippines

Location

Research Site

Singapore, 168583, Singapore

Location

Research Site

Singapore, 229899, Singapore

Location

Research Site

Singapore, 258499, Singapore

Location

Research Site

Singapore, 308433, Singapore

Location

Research Site

Bangkok, 10330, Thailand

Location

Research Site

Bangkok, 10400, Thailand

Location

Research Site

Bangkok, 10700, Thailand

Location

Research Site

Hanoi, Vietnam

Location

Research Site

Ho Chi Minh City, Vietnam

Location

Research Site

Huế, Vietnam

Location

MeSH Terms

Conditions

Breast Neoplasms

Condition Hierarchy (Ancestors)

Neoplasms by SiteNeoplasmsBreast DiseasesSkin DiseasesSkin and Connective Tissue Diseases

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
RETROSPECTIVE
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

November 9, 2023

First Posted

November 14, 2023

Study Start

April 23, 2024

Primary Completion

May 19, 2025

Study Completion

May 19, 2025

Last Updated

September 18, 2025

Record last verified: 2025-09

Data Sharing

IPD Sharing
Will share

Qualified researchers can request access to anonymized individual patient-level data from AstraZeneca group of companies sponsored clinical trials via the request portal. All request will be evaluated as per the AZ disclosure commitment: https://astrazenecagrouptrials.pharmacm.com/ST/Submission/Disclosure. Yes, indicates that AZ are accepting requests for IPD, but this does not mean all requests will be shared.

Shared Documents
STUDY PROTOCOL, SAP
Time Frame
AstraZeneca will meet or exceed data availability as per the commitments made to the EFPIA Pharma Data Sharing Principles. For details of our timelines, please rerefer to our disclosure commitment at https://astrazenecagrouptrials.pharmacm.com/ST/Submission/Disclosure.
Access Criteria
When a request has been approved AstraZeneca will provide access to the deidentified individual patient-level data in an approved sponsored tool . Signed Data Sharing Agreement (non-negotiable contract for data accessors) must be in place before accessing requested information. Additionally, all users will need to accept the terms and conditions of the SAS MSE to gain access. For additional details, please review the Disclosure Statements at https://astrazenecagrouptrials.pharmacm.com/ST/Submission/Disclosure.
More information

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