Effect of OMEGA 3 Fatty Acids on Lactation Period

NCT06111378 | Completed

• 1 other identifier: MCBU_lactation
• Study Type: observational
• Target: 32
• Locations: 1 country
• Sites: 1

Brief Summary

The World Health Organization (WHO) and the United Nations Children's Fund (UNICEF) recommend that babies be exclusively breastfed for the first 6 months from birth. The composition of breast milk is dynamic and may vary according to the content of maternal nutrition. Maternal nutrition, on the other hand, determines the quality of breast milk necessary for the adequate growth and health of infants. For this reason, the content of the maternal diet is important during the lactation period, which is the critical stage that can affect the development of infants. Omega 3 fatty acids are essential unsaturated fatty acids that are not synthesized by the human body and must be obtained through diet. The polyunsaturated fatty acids (PUFAs) most abundant in the diet are short-chain omega 3 alpha linolenic acid (ALA) and omega 6 linoleic acid (LA), which are mostly found in vegetable oils. ALA is a plant-derived omega 3 fatty acid found in flaxseed, walnut, canola and soybean oils. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are found in marine sources such as seafood and fish oils. Today, the use of Omega 3 fatty acid supplements is increasing during the lactation period. However, consumption of omega 3 fatty acid sources during pregnancy or lactation is thought to be an important factor in preventing cancer risk. In our study, the investigators aimed to compare the maternal diet and the consumption of fish oil, flaxseed oil and walnut oil supplements during the lactation period, to compare the amount of prolactin and DHA in the milk composition and to compare the protective effects against breast cancer with cancer stem cell markers. For this study, 32 healthy Wistar pregnant rats, weighing 200-250 g, 8-10 weeks pregnant and not included in any experiments, will be used. Supplementary oils will be administered by oral gavage, considering the 0.5 g/kg body weight of the pregnant rats according to the groups they are assigned to, during the entire standard lactation period (day 21), considering the day of birth of the pregnant rats as the 0th day of lactation. Mother rats will be sacrificed under anesthesia at the end of the 21st day of lactation, mammary tissues will be taken into appropriate solutions and histopathological and immunohistochemical examinations will be performed. In addition, cardiac blood will be drawn and blood and tissue biochemical analyzes will be performed.

Conditions

Lactation Induced

Keywords

lactation; omega 3 fatty acid; fish oil; flaxseed oil; walnut oil; breast cancer

Outcome Measures

Primary Outcomes (1)

• Analysis of the Effects of OMEGA 3 Fatty Acids on Lactation

  A Comparative Investigation of the maternal diet and the consumption of fish oil, flaxseed oil and walnut oil supplements during the lactation period. A Comparative Investigation of amount of prolactin and DHA in the milk composition and to compare the protective effects against breast cancer with cancer stem cell markers.

  Baseline

Study Arms (5)

Group 1:

control group (n:7)

Group 2:

fish oil group (n:7)

Dietary Supplement: fish oil

Group 3:

linseed oil group (n:7)

Dietary Supplement: linseed oil

Group 4:

walnut oil group (n:7)

Dietary Supplement: walnut oil

Group 5:

sham group (non-lactating rats) (n:4)

Interventions

fish oil DIETARY_SUPPLEMENT

fish oil

Group 2:

linseed oil DIETARY_SUPPLEMENT

linseed oil

Group 3:

walnut oil DIETARY_SUPPLEMENT

walnut oil

Group 4:

Eligibility Criteria

• Age: 8 Weeks - 10 Weeks
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Child (0-17)
• Sampling Method: Probability Sample

Study Population

pregnant rats unpregnant female rats

You may qualify if:

• healthy rats

You may not qualify if:

• patient rats
• rats that cannot be gavaged

Sponsors & Collaborators

• Celal Bayar University: lead

Study Sites (1)

Manisa Celal Bayar University

Yunusemre, Mani̇sa, Turkey (Türkiye)

Location

Related Publications (1)

• Mutlu Durgut I, Yesil Sarsmaz H, Gurgen SG. A Comparative Study on the Effect of Omega-3 Fatty Acids on Lactation. Mol Nutr Food Res. 2026 Jan;70(2):e70393. doi: 10.1002/mnfr.70393.

  PMID: 41572962 DERIVED

Biospecimen

Retention: SAMPLES WITH DNA

breast tissues cells

MeSH Terms

Conditions

Breast Feeding; Breast Neoplasms

Interventions

Fish Oils; Linseed Oil

Condition Hierarchy (Ancestors)

Feeding Behavior; Behavior; Neoplasms by Site; Neoplasms; Breast Diseases; Skin Diseases; Skin and Connective Tissue Diseases

Intervention Hierarchy (Ancestors)

Oils; Lipids; Fats, Unsaturated; Fats; Plant Oils; Plant Preparations; Biological Products; Complex Mixtures

Study Design

• Study Type: observational
• Observational Model: CASE CROSSOVER
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Asistant Prof.

Study Record Dates

• First Submitted: December 1, 2022
• First Posted: November 1, 2023
• Study Start: June 15, 2022
• Primary Completion: December 17, 2022
• Study Completion: August 31, 2023
• Last Updated: November 1, 2023

Record last verified: 2022-12

Locations

TU (1)