Impact of Canola Protein Processing on Plasma Amino Acid Responses

NCT06058403 | Completed DMC

• 1 other identifier: NL83369.068.23 / METC 23-025
• Study Type: interventional
• Target: 15
• Locations: 1 country
• Sites: 1

Brief Summary

Muscle tissue consists of proteins. These proteins are built up of small building blocks: amino acids. By consuming enough protein through the diet, the body is provided with enough amino acids to facilitate muscle protein building. Providing the growing world population with sufficient animal-derived protein is a challenge. Plant proteins can be produced on a more sustainable commercial scale than conventional animal-derived proteins and therefore can contribute to feeding our future population. Canola protein is a protein that is derived from rapeseed. The composition of canola seems to be comparable to that of other high-quality animal based protein sources. However, the collection of canola protein from rapeseed occurs in a special way. These treatment processes might affect canola protein digestion. The goal of this study is to investigate the most optimal way of canola protein processing on blood plasma amino acid responses. Primary objective: To assess the impact of canola protein processing on 5h postprandial plasma total amino acid incremental area under the curve (iAUC) in vivo in healthy young females. Hypothesis: it is hypothesized that the ingestion of 20g processed canola will result in greater 5h postprandial plasma total amino acid iAUC in vivo in healthy young females, when compared to the ingestion of 20g native canola protein isolate.

Conditions

Protein Metabolism Disorder

Keywords

Dietary protein; Plant protein; Protein processing; Protein digestion

Outcome Measures

Primary Outcomes (1)

• Total amino acid availability

  The total amino acid availability is expressed as the incremental area under the curve (iAUC)

  0 - 5 hours after drink ingestion

Secondary Outcomes (7)

• Postprandial plasma total amino acid concentrations

  0 - 5 hours after drink ingestion

• Postprandial plasma individual amino acid concentrations

  0 - 5 hours after drink ingestion

• Peak plasma amino acid concentrations

  0 - 5 hours after drink ingestion

• Time to peak plasma amino acid concentrations

  0 - 5 hours after drink ingestion

• iAUC essential amino acids concentrations

  0 - 5 hours after drink ingestion

Other Outcomes (12)

• Age in years

  Baseline at screening

• Bodymass in kg

  Baseline at screening

• Height in m

  Baseline at screening

Study Arms (4)

Native canola protein

EXPERIMENTAL

20g of canola protein

Dietary Supplement: 20g native canola protein drink ingestion

Processed canola protein #1

EXPERIMENTAL

20g of processed canola #1

Dietary Supplement: 20g processed canola protein #1 drink ingestion

Processed canola protein #2

EXPERIMENTAL

20g of processed canola #2

Dietary Supplement: 20g processed canola protein #2 drink ingestion

Whey protein

ACTIVE COMPARATOR

20g of whey protein

Dietary Supplement: 20g whey protein drink ingestion

Interventions

20g whey protein drink ingestion DIETARY_SUPPLEMENT

Ingestion of a 300 mL drink containing 20g whey protein

Also known as: Supplement

Whey protein

20g native canola protein drink ingestion DIETARY_SUPPLEMENT

Ingestion of a 300 mL drink containing 20g native canola protein

Also known as: Supplement

Native canola protein

20g processed canola protein #1 drink ingestion DIETARY_SUPPLEMENT

Ingestion of a 300 mL drink containing 20g processed canola protein #1

Also known as: Supplement

Processed canola protein #1

20g processed canola protein #2 drink ingestion DIETARY_SUPPLEMENT

Ingestion of a 300 mL drink containing 20g processed canola protein #2

Also known as: Supplement

Processed canola protein #2

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Female sex
• Aged between 18 and 35 y inclusive
• BMI between 18.5 and 30 kg/m2

You may not qualify if:

• Intolerant to milk lactose
• Allergic to milk protein / products
• Mustard allergy
• Vegan lifestyle
• Participating in a structured (progressive) exercise program
• Smoking regularly
• Diagnosed GI tract disorders or diseases
• Diagnosed musculoskeletal disorders
• Diagnosed metabolic disorders (e.g., diabetes)
• Hypertension (blood pressure above 140/90 mmHg)
• Donated blood 2 months prior to test day
• Pregnant
• Using third generation oral contraceptives
• Use of any medications known to affect protein metabolism (i.e., corticosteroids, non-steroidal anti-inflammatories).
• Chronic use of gastric acid suppressing medication as this impairs protein digestion (i.e., proton pump inhibitors, H2-antagonists)

Sponsors & Collaborators

• Maastricht University Medical Center: lead
• DSM Food Specialties: collaborator

Study Sites (1)

Maastricht University Medical Centre+

Maastricht, Limburg, 6229ER, Netherlands

Location

Related Publications (1)

• Boot N, Hermans WJH, Warnke I, Overman A, van Kranenburg JMX, Senden JM, Verdijk LB, van Loon LJC. Evaluation of processing of canola protein isolate on postprandial plasma amino acid profiles in healthy, young females. Amino Acids. 2025 Oct 17;57(1):50. doi: 10.1007/s00726-025-03482-1.

  PMID: 41107640 DERIVED

MeSH Terms

Interventions

Dietary Supplements

Intervention Hierarchy (Ancestors)

Food; Diet, Food, and Nutrition; Physiological Phenomena; Food and Beverages

Study Officials

• Luc van Loon, PhD

  Maastricht University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: Randomized, controlled, double-blind, cross-over intervention trial

Study Record Dates

• First Submitted: September 5, 2023
• First Posted: September 28, 2023
• Study Start: October 3, 2023
• Primary Completion: May 27, 2024
• Study Completion: May 27, 2024
• Last Updated: March 30, 2026

Record last verified: 2023-10

Data Sharing

• IPD Sharing: Will not share

Locations

NL (1)