NCT06052436

Brief Summary

The investigators developed a GMP protocol to isolate Treg cells from thymic tissue (thyTreg). The thyTreg cells are being evaluated in a Phase I/II clinical trial to evaluate the safety and efficacy of the adoptive transfer of autologous thyTreg to prevent rejection in heart transplant children (NCT04924491), with preliminary results indicating the feasibility and safety of the therapy. In addition, thyTreg cells have shown low immunogenicity in the pre-clinical setting, indicating that allogeneic use of these thyTreg cells (allo-thyTreg) would have a low risk of adverse effects. These thyTreg cells could inhibit an excessive inflammation in SARS-CoV-2 infection, or ameliorate the immunological affection underlying Acute respiratory distress syndrome, improving life-threatening manifestations, restoring immune balance, and protecting affected tissues. This clinical trial is an open-label Sequential Parallel Group Phase I/II study to evaluate the safety and efficacy of allogeneic thymus derived Tregs (thyTreg) (thyTreg) in controlling the immune dysregulation associated with SARS-CoV-2 infection and/or Acute Respiratory Distress Syndrome.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
24

participants targeted

Target at P25-P50 for phase_1

Timeline
20mo left

Started Jun 2023

Longer than P75 for phase_1

Geographic Reach
1 country

1 active site

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress63%
Jun 2023Dec 2027

Study Start

First participant enrolled

June 27, 2023

Completed
3 months until next milestone

First Submitted

Initial submission to the registry

September 12, 2023

Completed
13 days until next milestone

First Posted

Study publicly available on registry

September 25, 2023

Completed
4.3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 31, 2027

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

December 31, 2027

Last Updated

February 3, 2025

Status Verified

January 1, 2025

Enrollment Period

4.5 years

First QC Date

September 12, 2023

Last Update Submit

January 30, 2025

Conditions

Keywords

Immune HyperactivationRegulatory T cellARDSCOVID-19Advanced therapyImmunotherapyTh1-Th2 Balance

Outcome Measures

Primary Outcomes (1)

  • 1. Incidence of infusion-related adverse events (safety) by type, frequency, severity, and causality

    24 months

Secondary Outcomes (16)

  • Length of intensive care unit stay

    24 months

  • Oxygenation improvement as assessed using PaO2/FiO2 and/or SaO2/FiO2

    24 months

  • Change in clinical status as assessed using Sequential Organ Failure Assessment Score

    24 months

  • Change in clinical status as assessed using Acute Physiology and Chronic Health disease Classification System (APACHE) III

    24 months

  • Change in clinical status as assessed using Barthel score

    24 months

  • +11 more secondary outcomes

Study Arms (4)

Phase A: 5.000.000 thyTreg /kg

EXPERIMENTAL

Allogeneic thyTreg 5.000.000

Biological: Allogeneic thyTreg 5.000.000

Phase A: standard of care

NO INTERVENTION

Standard of care

Phase B: 10.000.000 thyTreg /kg

EXPERIMENTAL

Allogeneic thyTreg 10.000.000

Biological: Allogeneic thyTreg 10.000.000

Phase B: standard of care

NO INTERVENTION

Standard of care

Interventions

Treg lymphocytic cells, differentiated, allogeneic, of thymic tissue, expanded and stimulated with Interleukin (IL-) 2 (thyTreg)

Also known as: Allogeneic thyTreg cells
Phase A: 5.000.000 thyTreg /kg

Treg lymphocytic cells, differentiated, allogeneic, of thymic tissue, expanded and stimulated with Interleukin (IL-) 2 (thyTreg)

Also known as: Allogeneic thyTreg cells
Phase B: 10.000.000 thyTreg /kg

Eligibility Criteria

Age18 Years - 65 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Patient over 18 to 65 years of age
  • Patient Informed and non-opposed to the research by his medical doctor during hospitalization
  • Patient with clinical, radiological, gasometric and immunological criteria defined as:
  • Acute respiratory failure secondary to acute lung injury of noncardiogenic cause
  • Pulmonary abnormalities compatible with bilateral alveoloinsterstitial infiltrates by chest imaging (radiograph or scan)
  • PaO2/FiO2≤ 300 Presence of at least one of the following markers of inflammation: IL6 \> 40 pg/ml or ferritin \>300 ng/ml or CRP \>3 mg/dl or increasing over the last 24 hours

You may not qualify if:

  • Pregnancy or breast feeding
  • Body mass index \>35
  • Patients not expected to survive 48 hours after enrolment based on clinical assessment
  • Patients with an extracorporeal respiratory support
  • Neutropenia (absolute neutrophil count \<1000/uL)
  • Thrombocytopenia (absolute neutrophil count \<50000/uL)
  • Positive serology for HBV, HCV, or HIV at Screening
  • Life expectancy of less than 6 months due to other pathologies
  • Patients with a history of autoimmune diseases
  • Patients with a history of hematopoietic neoplasia or oncology disease
  • Patients with a history of hematopoietic or solid organ transplant
  • Patients with a congenital or induced immunodeficiency
  • Patients received thymoglobulin, basiliximab or any anti-T-cell therapies within 6 moths prior to the screening visit
  • Patients received other cell therapy in the last 12 months
  • Patients received intravenous immunoglobulin (IVIg) within 5 moths prior to the screening visit
  • +1 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Hospital General Universitario Gregorio Marañon

Madrid, Madrid, 28007, Spain

RECRUITING

MeSH Terms

Conditions

Systemic Inflammatory Response SyndromeCOVID-19

Condition Hierarchy (Ancestors)

InflammationPathologic ProcessesPathological Conditions, Signs and SymptomsShockPneumonia, ViralPneumoniaRespiratory Tract InfectionsInfectionsVirus DiseasesCoronavirus InfectionsCoronaviridae InfectionsNidovirales InfectionsRNA Virus InfectionsLung DiseasesRespiratory Tract Diseases

Study Officials

  • Rafael Correa-Rocha, PhD

    Hospital General Universitario Gregorio Marañon

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Marta Martínez-Bonet, PhD

CONTACT

Diana Hernández Flórez, PhD

CONTACT

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
FACTORIAL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Principal Investigator

Study Record Dates

First Submitted

September 12, 2023

First Posted

September 25, 2023

Study Start

June 27, 2023

Primary Completion (Estimated)

December 31, 2027

Study Completion (Estimated)

December 31, 2027

Last Updated

February 3, 2025

Record last verified: 2025-01

Locations