The Effect of Oral Intake of Animal and Plant Proteins on the Metabolism in Healthy Women

NCT06045611 | Active Not Recruiting

• 1 other identifier: Dnr 2023-03852-01
• Study Type: interventional
• Target: 28
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this interventional study is to learn about the effects of different proteins (from animals or plants) on the metabolism in healthy, normal weight women between 18-45 year who are not on hormonal birth-control. The main questions the study aims to answer are:

1. 1.How do different proteins affect biological markers of the metabolism and which protein has the strongest effect?
2. 2.How are the different proteins digested and taken up from our body?

Conditions

Healthy

Keywords

Protein; Plant-based protein; Metabolism; Somatotrophic Axis

Outcome Measures

Primary Outcomes (1)

• Release of Growth Hormone

  The primary outcome is defined as the postprandial release of growth hormone during three hours after oral ingestion of plant-protein test drinks vs. an animal-protein test drink measured as incremental area under the curve. Growth hormone will be measured in blood samples.

  0-3 hours after consumption of test drink (on all 4 study days)

Secondary Outcomes (2)

• Release of Hormones Related to the Somatotrophic Axis

  0-3 hours after consumption of test drink (on all 4 study days)

• Kinetics of Amino Acid Uptake

  0-3 hours after consumption of test drink (on all 4 study days)

Study Arms (4)

Isolated Pea Protein/Isolated Whey Protein/Isolated Faba Bean Protein/Placebo

EXPERIMENTAL

* Study day 1: 0.6g isolated pea protein/kg/body weight + 400 ml water * Study day 2: 0.6g isolated whey protein/kg/body weight + 400 ml water * Study day 3: 0.6g isolated faba bean protein/kg/body weight + 400 ml water * Study day 4: 400 ml water The fat and energy content of the drinks will be adapted using a plant based oil

Dietary Supplement: Isolated Pea Protein; Dietary Supplement: Isolated Whey Protein; Dietary Supplement: Isolated Faba Bean Protein; Dietary Supplement: Placebo

Isolated Whey Protein/Placebo/Isolated Pea Protein/Isolated Faba Bean Protein

EXPERIMENTAL

* Study day 1: 0.6g isolated whey protein/kg/body weight + 400 ml water * Study day 2: 400 ml water * Study day 3: 0.6g isolated pea protein/kg/body weight + 400 ml water * Study day 4: 0.6g isolated faba bean protein/kg/body weight + 400 ml water The fat and energy content of the drinks will be adapted using a plant based oil

Dietary Supplement: Isolated Pea Protein; Dietary Supplement: Isolated Whey Protein; Dietary Supplement: Isolated Faba Bean Protein; Dietary Supplement: Placebo

Isolated Faba Bean Protein/Isolated Pea Protein/Placebo/Isolated Whey Protein

EXPERIMENTAL

* Study day 1: 0.6g isolated faba bean protein/kg/body weight + 400 ml water * Study day 2: 0.6g isolated pea protein/kg/body weight + 400 ml water * Study day 3: 400 ml water * Study day 4: 0.6g isolated whey protein/kg/body weight + 400 ml water The fat and energy content of the drinks will be adapted using a plant based oil

Dietary Supplement: Isolated Pea Protein; Dietary Supplement: Isolated Whey Protein; Dietary Supplement: Isolated Faba Bean Protein; Dietary Supplement: Placebo

Placebo/Isolated Faba Bean Protein/Isolated Whey Protein/Isolated Pea Protein

EXPERIMENTAL

* Study day 1: 400 ml water * Study day 2: 0.6g isolated faba bean protein/kg/body weight + 400 ml water * Study day 3: 0.6g isolated whey protein/kg/body weight + 400 ml water * Study day 4: 0.6g isolated pea protein/kg/body weight + 400 ml water The fat and energy content of the drinks will be adapted using a plant based oil

Dietary Supplement: Isolated Pea Protein; Dietary Supplement: Isolated Whey Protein; Dietary Supplement: Isolated Faba Bean Protein; Dietary Supplement: Placebo

Interventions

Isolated Pea Protein DIETARY_SUPPLEMENT

Participants will consume the protein supplement dissolved in water in an opaque mug wearing a nose clip.

Isolated Faba Bean Protein/Isolated Pea Protein/Placebo/Isolated Whey Protein; Isolated Pea Protein/Isolated Whey Protein/Isolated Faba Bean Protein/Placebo; Isolated Whey Protein/Placebo/Isolated Pea Protein/Isolated Faba Bean Protein; Placebo/Isolated Faba Bean Protein/Isolated Whey Protein/Isolated Pea Protein

Isolated Whey Protein DIETARY_SUPPLEMENT

Participants will consume the protein supplement dissolved in water in an opaque mug wearing a nose clip.

Isolated Faba Bean Protein/Isolated Pea Protein/Placebo/Isolated Whey Protein; Isolated Pea Protein/Isolated Whey Protein/Isolated Faba Bean Protein/Placebo; Isolated Whey Protein/Placebo/Isolated Pea Protein/Isolated Faba Bean Protein; Placebo/Isolated Faba Bean Protein/Isolated Whey Protein/Isolated Pea Protein

Isolated Faba Bean Protein DIETARY_SUPPLEMENT

Participants will consume the protein supplement dissolved in water in an opaque mug wearing a nose clip.

Isolated Faba Bean Protein/Isolated Pea Protein/Placebo/Isolated Whey Protein; Isolated Pea Protein/Isolated Whey Protein/Isolated Faba Bean Protein/Placebo; Isolated Whey Protein/Placebo/Isolated Pea Protein/Isolated Faba Bean Protein; Placebo/Isolated Faba Bean Protein/Isolated Whey Protein/Isolated Pea Protein

Placebo DIETARY_SUPPLEMENT

Participants will consume the placebo in an opaque mug wearing a nose clip.

Isolated Faba Bean Protein/Isolated Pea Protein/Placebo/Isolated Whey Protein; Isolated Pea Protein/Isolated Whey Protein/Isolated Faba Bean Protein/Placebo; Isolated Whey Protein/Placebo/Isolated Pea Protein/Isolated Faba Bean Protein; Placebo/Isolated Faba Bean Protein/Isolated Whey Protein/Isolated Pea Protein

Eligibility Criteria

• Age: 18 Years - 45 Years
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Females
• Age: 18-45 years
• Body mass index: 18.5 \< 25 kg/m2
• Signed informed consent

You may not qualify if:

• Use of hormonal contraceptives
• Pregnancy or breastfeeding
• Diabetes mellitus or any other metabolic disease that might affect the results of this study
• Gastric surgery that affects digestion and uptake of protein
• Any clinically significant present or past disease/condition and medication which in the investigator's opinion could interfere with the results of the trial

Sponsors & Collaborators

• Örebro University, Sweden: lead

Study Sites (1)

Örebro University

Örebro, 70182, Sweden

Location

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: September 13, 2023
• First Posted: September 21, 2023
• Study Start: October 13, 2023
• Primary Completion: October 24, 2024
• Study Completion: September 1, 2025
• Last Updated: August 14, 2025

Record last verified: 2025-08

Locations

SE (1)