The Potential Association of Long Non-coding RNA (NEAT1 and MALAT1) in the Process of Epithelial-mesenchymal Transition in Periodontitis

NCT05995067 | Unknown DMC

• 1 other identifier: lncRNA in periodontal disease
• Study Type: observational
• Target: 48
• Locations: 1 country
• Sites: 1

Brief Summary

Periodontitis is a polymicrobial, inflammatory condition affecting tooth-supporting tissues. It is characterized by a progressive loss of epithelial attachment and resorption of alveolar bone, which can lead to tooth loss. Epithelial-mesenchymal transition (EMT) has been proposed as a reversible process that shifts the cell phenotype from epithelial to mesenchymal-like and may be involved in the process of periodontal inflammation either completely or partially. Long non-coding RNAs (lncRNAs) are a large class of regulatory transcripts longer than 200 nucleotides lacking evident protein-coding potential. Studies have shown that lncRNA dysregulation plays key roles in human diseases, including cancer, by modulating the epithelial-mesenchymal transition (EMT). The involvement of lncRNAs markers in the pathogenesis of periodontal disease induced EMT has not been studied thoroughly and have created a gap in the knowledge.

Conditions

Periodontal Disease, AVDC Stage 3and 4; Epithelial Mesenchymal Transition; Long Noncoding RNA

Outcome Measures

Primary Outcomes (1)

• determining the relative expression of lncRNAs (NEAT1 and MALAT1) in healthy and diseased periodontal tissue.

  reverse transcription real time polymerase chain reaction

  one year

Secondary Outcomes (1)

• determine the expression of EMT-related genes (E-cadherin, Vimentin, β-catenin, and Snail1), in healthy and diseased tissues, and their correlation with lncRNAs and clinical parameters.

  one year

Study Arms (2)

control group

-healthy groups/without the condition

study group

-diseased group-/with the condition (periodontitis)

Other: periodontitis challenge

Interventions

periodontitis challenge OTHER

control will be compared to diseased group suffering from periodontal disease.

study group

Eligibility Criteria

• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Patients diagnosed with periodontitis, and healthy periodontal tissues will be recruited at the College of Dentistry/University of Baghdad and Al Sadir specialized dental center/Al-Risafa directorate/ Ministry of Health. No age or gender restriction.

You may qualify if:

• Patients with no history of systemic disease e.g., diabetes.
• Both males and females will be included in the study.
• Control group: patients fit the case definition of gingival health2, and confirmed by Histopathological inflammatory cell infiltration analysis.
• Periodontitis group: patients fit the case definition of periodontal disease referred for periodontal flap surgery (modified Widman's flap).
• Patients radiographically confirmed with infrabony pocket eligible for periodontal flap surgery.

You may not qualify if:

• Patients not willing to participate or consent.
• Patient taking medications that affect or modify immunity (e.g., corticosteroid), for the last 3 months.
• Patient taking NSAIDs for the last 3 months.
• Active smoker.

Sponsors & Collaborators

• Ali A Abdulkareem: lead

Study Sites (1)

saif mohammed T AL Mufti

Baghdad, 964, Iraq

Location

Biospecimen

Retention: SAMPLES WITH DNA

gingival tissue byproduct of resective periodontal surgery

MeSH Terms

Conditions

Periodontal Diseases

Condition Hierarchy (Ancestors)

Mouth Diseases; Stomatognathic Diseases

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: assistant professor- department of periodontics-college of dentistry- Baghdad university

Study Record Dates

• First Submitted: August 8, 2023
• First Posted: August 16, 2023
• Study Start: February 14, 2022
• Primary Completion: January 30, 2024
• Study Completion: January 30, 2024
• Last Updated: December 12, 2023

Record last verified: 2023-12

Locations

IR (1)