The Effect of Beetroot Juice Supplementation Dose on Neuromuscular Performance During Resistance Exercise

NCT05957835 | Unknown

• 1 other identifier: RT12
• Study Type: interventional
• Target: 18
• Locations: 1 country
• Sites: 1

Brief Summary

Dietary nitrate supplementation, administered in the form of nitrate-rich beetroot juice, has been shown to improve exercise performance and may have more pronounced effects on exercise that requires high-power and high-velocity muscle contraction such as weightlifting and body-mass resisted exercise (i.e, resistance exercise). However, limited data are available that have examined the potential performance enhancing effect of beetroot juice in resistance exercise. Moreover, there is no information on the dose of nitrate required for performance enhancing effects during resistance exercise which limits its widespread use to enhance performance. Therefore, the investigators will assess if supplementing the diet with nitrate-rich beetroot juice impacts resistance exercise performance (e.g., power, velocity), when compared to supplementing the diet with nitrate-depleted beetroot juice (i.e. placebo). The investigators will also investigate the dose-response effect of beetroot juice on resistance exercise performance by providing a low, moderate, and high nitrate dose of concentrated beetroot juice. The results of this study will improve understanding of whether beetroot juice influences resistance exercise performance and will provide insight on whether different doses of beetroot juice influence the efficacy of nitrate supplementation on resistance exercise performance. Together, these data will guide supplementation strategies for enhancing resistance exercise performance.

Conditions

High Nitrate Dose; Moderate Nitrate Dose; Low Nitrate Dose; Nitrate-depleted Dose

Outcome Measures

Primary Outcomes (15)

• Plasma nitrate

  Plasma nitrate concentrations in each condition from blood samples draw at rest prior to exercise, and analyzed using gas phase chemiluminescence

  Through study completion, at least 1 year

• Plasma nitrite

  Plasma nitrite concentrations in each condition from blood samples draw at rest prior to exercise, and analyzed using gas phase chemiluminescence

  Through study completion, at least 1 year

• Neuromuscular performance during countermovement jumps

  Peak force will be measured using a force place.

  Through study completion, at least 1 year

• Neuromuscular performance during countermovement jumps

  average propulsion force will be measured using a force place.

  Through study completion, at least 1 year

• Neuromuscular performance during countermovement jumps

  rate of power development will be measured using a force place.

  Through study completion, at least 1 year

• Neuromuscular performance during countermovement jumps

  peak positive power will be measured using a force place.

  Through study completion, at least 1 year

• Neuromuscular performance during countermovement jumps

  concentric mean power will be measured using a force place.

  Through study completion, at least 1 year

• Neuromuscular performance during countermovement jumps

  jump height will be measured using a force place.

  Through study completion, at least 1 year

• Neuromuscular performance during countermovement jumps

  takeoff velocity will be measured using a force place.

  Through study completion, at least 1 year

• Neuromuscular performance during countermovement jumps

  flight time will be measured using a force place.

  Through study completion, at least 1 year

• Neuromuscular performance during countermovement jumps

  Peak force, average propulsion force, rate of power development, peak positive power, concentric mean power, jump height, takeoff velocity, flight time will be measured using a force place.

  Through study completion, at least 1 year

• Peak power

  Power will be measured using a linear transducer with the highest value will be recorded for peak power

  Through study completion, at least 1 year

• Mean power

  Power will be measured using a linear transducer and will be averaged across sets for the determination of mean power

  Through study completion, at least 1 year

• Mean velocity

  Velocity will be measured using a linear transducer and will be averaged across sets for the determination of mean velocity

  Through study completion, at least 1 year

• Peak velocity

  Velocity will be measured using a linear transducer the highest value will be retained for peak velocity

  Through study completion, at least 1 year

Secondary Outcomes (1)

• Mood

  Through study completion, at least 1 year

Study Arms (4)

BR-HIGH

EXPERIMENTAL

Participants will ingest an elevated dose of nitrate 2.5 hours prior to exercise.

Dietary Supplement: BR-HIGH

BR-MOD

EXPERIMENTAL

Participants will ingest a moderate dose of nitrate 2.5 hours prior to exercise.

Dietary Supplement: BR-MOD

BR-LOW

EXPERIMENTAL

Participants will ingest a low dose of nitrate 2.5 hours prior to exercise.

Dietary Supplement: BR-LOW

PL

PLACEBO COMPARATOR

Participants will ingest a negligible amount of nitrate 2.5 hours prior to exercise.

Dietary Supplement: PL

Interventions

BR-HIGH DIETARY_SUPPLEMENT

4 x nitrate-rich beetroot juice shots (26 mmol of nitrate)

BR-HIGH

BR-MOD DIETARY_SUPPLEMENT

2 x nitrate-rich beetroot juice shots + 2 x nitrate-depleted beetroot juice shot (12 mmol of nitrate)

BR-MOD

BR-LOW DIETARY_SUPPLEMENT

1 x nitrate-rich beetroot juice shot + 3 x nitrate-depleted beetroot juice shots (6 mmol of nitrate)

BR-LOW

PL DIETARY_SUPPLEMENT

4 x nitrate-depleted beetroot juice shots (0 mmol of nitrate)

PL

Eligibility Criteria

• Age: 18 Years - 45 Years
• Gender Eligibility Details: Females were excluded given that sex-differences in the physiological responses to nitrate ingestion may exist and that including the appropriate controls (i.e., testing only during the early follicular phase) would have been unfeasible logistically.
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Recreationally active males who performed resistance exercise at least twice weekly for the last two years

You may not qualify if:

• individuals with contraindications to exercise, cardiometabolic disease, currently consuming dietary supplements containing caffeine, sodium bicarbonate, creatine, beta-alanine, and/or NO precursor supplements (i.e., NO3-, arginine, citrulline, antioxidants), females, and smokers

Sponsors & Collaborators

• Pepperdine University: lead

Study Sites (1)

Pepperdine University

Malibu, California, 90263, United States

RECRUITING

Study Officials

• Rachel Tan, PhD

  Pepperdine University

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Rachel Tan, PhD

CONTACT

3105067041; rachel.tan@pepperdine.edu

Adam Pennell, PhD

CONTACT

3105064162; adam.pennell@pepperdine.edu

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, OUTCOMES ASSESSOR
• Masking Details: The lead researcher, data collectors, and participants were blinded to the conditions. The distribution of supplements for each condition was performed by a researcher that was not formally involved in data collection processes, thereby limiting the potential of bias.
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor of Sports Medicine

Model Details: In a double-blind, randomised, crossover design, participants were assigned to four experimental trials to consume a total of 4 beetroot juice shots during each condition: (a) 4 x 70 ml of nitrate-depleted beetroot juice for placebo (PL), (b) 1 x 70 ml of nitrate-rich beetroot juice 3 x 70 ml nitrate-depleted beetroot juice for a low dose of nitrate-rich beetroot juice (BR-LOW; \~6.5 mmol of nitrate total), (c) 2 x 70 of nitrate-rich beetroot juice and 2 x 70ml of nitrate depleted beetroot juice for a moderate dose of nitrate-rich beetroot juice (BR-MOD; \~13 mmol of nitrate total), and (d) 4 x 70 ml of nitrate-rich beetroot juice for an elevated dose of nitrate-rich beetroot juice (BR-HIGH; \~26 mmol of nitrate total).

Study Record Dates

• First Submitted: June 28, 2023
• First Posted: July 24, 2023
• Study Start: May 23, 2023
• Primary Completion: April 28, 2024
• Study Completion: June 30, 2024
• Last Updated: July 24, 2023

Record last verified: 2023-06

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)