NCT05888532

Brief Summary

This phase I/II clinical trial evaluates if using a radiotracer targeting granzyme B, 64-copper granzyme targeting restricted interaction peptide specific to family member B (64 Cu-GRIP B) with positron emission tomography (PET) imaging can be safe and useful for detecting granzyme B (GrB) in patients with advanced cancers that has spread to nearby tissue or lymph nodes (advanced). Granzyme B (GrB) is a biomarker produced by immune cells in response to immunotherapy, which may highlight tumors that are more likely to respond to treatment. The study population is focused on genitourinary (GU) malignancies, including renal cell and urothelial cancer, two tumor types with high mutational burden and tumor infiltrating lymphocytes compared to other tumor types, and have a predictable response rate at the population level to immune checkpoint inhibitors. The information gained from this trial may allow researchers to develop future trials where 64Cu-GRIP B PET may serve as a biomarker to monitor early response to immunomodulatory therapies which are used to stimulate or suppress the immune system and may help the body fight cancer.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
91

participants targeted

Target at P75+ for phase_1 prostate-cancer

Timeline
9mo left

Started May 2023

Geographic Reach
1 country

1 active site

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress80%
May 2023Jan 2027

First Submitted

Initial submission to the registry

May 24, 2023

Completed
1 day until next milestone

Study Start

First participant enrolled

May 25, 2023

Completed
11 days until next milestone

First Posted

Study publicly available on registry

June 5, 2023

Completed
3.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 31, 2026

Expected
1 month until next milestone

Study Completion

Last participant's last visit for all outcomes

January 31, 2027

Last Updated

March 17, 2026

Status Verified

March 1, 2026

Enrollment Period

3.6 years

First QC Date

May 24, 2023

Last Update Submit

March 13, 2026

Conditions

Keywords

Imaging StudyRadiotracerGranzyme B

Outcome Measures

Primary Outcomes (11)

  • Frequency of treatment-emergent adverse events (Cohort A)

    For Cohort A, the frequency and severity of adverse events following 64Cu-GRIP B injection will be descriptively reported, using NCI Common Terminology Criteria for Adverse Events (CTCAE) version 5.0

    Up to 8 weeks

  • Percent of injected activity (Cohort A)

    For Cohort A, the tracer kinetics is measured in the organs and total-body, and the % of injected activity for each time point will be recorded for participants in Cohort A. This information is used as input for organ and whole-body effective dose calculation using Organ Level INternal Dose Assessment/EXponential Modeling (OLINDA/EXM). This will provide the data of whole-body effective dose (millisievert (mSv)/megabecquerels (MBq)), and organ doses.

    Up to 8 weeks

  • Time to maximum observed concentration (Tmax) (Cohort A)

    Pharmacokinetic (PK) parameters for participants in Cohort A derived from plasma will be calculated using a non-compartmental approach with a log-linear terminal assumption for up to a possible 5 time points. A custom software package or a commercial software package like Phoenix WinNonlin will be used to compute the time it takes for a drug to reach the maximum concentration (Cmax) after administration of a drug that needs to be absorbed.

    Up to 8 weeks

  • Maximum observed concentration (Cmax) (Cohort A)

    PK parameters for participants in Cohort A derived from plasma will be calculated using a non-compartmental approach with a log-linear terminal assumption for up to a possible 5 time points. A custom software package or a commercial software package like Phoenix WinNonlin will be used to compute the maximum concentration (Cmax) after administration of a drug that needs to be absorbed.

    Up to 8 weeks

  • Area under the concentration-time curve (AUC) (Cohort A)

    PK parameters for participants in Cohort A derived from plasma will be calculated using a non-compartmental approach with a log-linear terminal assumption for up to a possible 5 time points. A custom software package or a commercial software package like Phoenix WinNonlin will be used to compute the area under the concentration-time curve (AUC) from hour 0 to the last measurable concentration (AUC0-t; min\*unit/mL)

    Up to 8 weeks

  • AUC extrapolated to infinity (Cohort A)

    PK parameters for participants in Cohort A derived from plasma will be calculated using a non-compartmental approach with a log-linear terminal assumption for up to a possible 5 time points. A custom software package or a commercial software package like Phoenix WinNonlin will be used to compute the AUC extrapolated to infinity (AUC0-∞; min\*unit/mL)

    Up to 8 weeks

  • Median clearance (Cohort A)

    PK parameters for participants in Cohort A derived from plasma will be calculated using a non-compartmental approach with a log-linear terminal assumption for up to a possible 5 time points. A custom software package or a commercial software package like Phoenix WinNonlin will be used to compute the volume of plasma which is completely cleared of a substance per minute (mL/min).

    Up to 8 weeks

  • Apparent terminal elimination rate constant (Cohort A)

    PK parameters for participants in Cohort A derived from plasma will be calculated using a non-compartmental approach with a log-linear terminal assumption for up to a possible 5 time points. A custom software package or a commercial software package like Phoenix WinNonlin will be used to compute the apparent terminal elimination rate constant.

    Up to 8 weeks

  • Apparent terminal elimination half-life (Cohort A)

    PK parameters for participants in Cohort A derived from plasma will be calculated using a non-compartmental approach with a log-linear terminal assumption for up to a possible 5 time points. A custom software package or a commercial software package like Phoenix WinNonlin will be used to compute apparent terminal elimination half-life (t1/2; min).

    Up to 8 weeks

  • Change in SUVmax (Cohorts B, C, and D)

    For Cohorts B, C and D, descriptive statistics will be used to summarize the change in SUVmax from baseline to 8 weeks at lesion level for participants in Cohorts B \& C.

    Up to 8 weeks

  • Change in SUVmax/SUVave (Cohorts B, C, and D)

    For Cohorts B, C and D, descriptive statistics will be used to summarize the change in the ratio of SUVmax/SUVave from baseline to 8 weeks at lesion level for participants in Cohorts B \& C.

    Up to 8 weeks

Secondary Outcomes (9)

  • Frequency of treatment-emergent adverse events (Cohorts B, C, and D)

    Up to 8 weeks

  • Mean SUVmax in metastatic lesions by disease site (Cohorts B, C and D)

    Up to 2 years

  • Percent of lesions detected for metastatic participants (Cohorts B, C and D)

    Up to 8 weeks

  • Median change in SUVmax from baseline with reported immune-related adverse event (Cohort B)

    Up to 2 years

  • Median change in SUVmax-ave from baseline with reported immune-related adverse event (Cohorts B)

    Up to 2 years

  • +4 more secondary outcomes

Study Arms (4)

Cohort A: 64Cu-GRIP B, Solid Tumor Malignancy participants

EXPERIMENTAL

Participants with solid tumor malignancies (3 males, 3 females), dosimetry calculation will be performed by obtaining whole body (vertex to thighs) PET images up to five time points from 0.5 to 24 hours post 64Cu-GRIP B injections. An additional intravenous line will be placed in the contra-lateral arm to collect blood for this group.

Drug: Copper-64 labeled Granzyme B (64Cu-GRIP B)Procedure: Positron Emission Tomography (PET)

Cohort B: 64Cu-GRIP B, RCC and UC participants

EXPERIMENTAL

Participants with renal cell and urothelial carcinoma will have longitudinal imaging performed prior to treatment outside of this study with anti-programmed death-1 (PD-1)/anti-PD-1 ligand 1 (PD-L1) blockade (with or without concomitant anti-CTLA4 treatment), after 8 weeks of checkpoint blockade, and again at the time of disease progression by Response Evaluation Criteria in Solid Tumors (RECIST) 1.1.

Drug: Copper-64 labeled Granzyme B (64Cu-GRIP B)Procedure: Positron Emission Tomography (PET)

Cohort C: 64Cu-GRIP B, mCRPC participants

EXPERIMENTAL

Participants with metastatic castration resistant prostate cancer (mCRPC)) will have longitudinal imaging performed prior to treatment outside of this study, 8 weeks following initiation of treatment outside of this study, and at the time of disease progression by Prostate Cancer Working Group 3 (PCWG3) criteria.

Drug: Copper-64 labeled Granzyme B (64Cu-GRIP B)Procedure: Positron Emission Tomography (PET)

Cohort D: 64Cu-GRIP B, Advanced malignancies

EXPERIMENTAL

participants with solid tumor malignancies will have longitudinal imaging performed prior to treatment outside of this study, 8 weeks following initiation of treatment, and the opportunity to have an optional scan at the time of progression.

Drug: Copper-64 labeled Granzyme B (64Cu-GRIP B)Procedure: Positron Emission Tomography (PET)

Interventions

Given IV prior to imaging

Also known as: 64Cu-GRIP B, 64Cu-labeled GRIP B
Cohort A: 64Cu-GRIP B, Solid Tumor Malignancy participantsCohort B: 64Cu-GRIP B, RCC and UC participantsCohort C: 64Cu-GRIP B, mCRPC participantsCohort D: 64Cu-GRIP B, Advanced malignancies

Imaging procedure

Also known as: Positron Emission Tomography, PET, PET Scan
Cohort A: 64Cu-GRIP B, Solid Tumor Malignancy participantsCohort B: 64Cu-GRIP B, RCC and UC participantsCohort C: 64Cu-GRIP B, mCRPC participantsCohort D: 64Cu-GRIP B, Advanced malignancies

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Disease characteristics by cohort, as defined by:
  • Cohort A:
  • Histologically-confirmed metastatic solid tumor malignancy (3 Male, 3 Female)
  • Locally advanced or metastatic disease on conventional imaging
  • Cohort B:
  • Histologically-confirmed metastatic renal cell carcinoma (any histologic sub-type) or urothelial carcinoma
  • Locally advanced or metastatic disease on conventional imaging
  • Cohort C:
  • Histologically-confirmed prostate adenocarcinoma
  • Metastatic castration resistant prostate cancer by Prostate Cancer Clinical Trials Working Group 3 (PCWG3) criteria
  • Planned treatment with immune checkpoint inhibitor (Cohorts B and C only)
  • Willing to undergo paired tumor biopsies and has safely accessible bone or soft tissue lesion (Cohorts B and C only)
  • The subject is able and willing to comply with study procedures and provide signed and dated informed consent.
  • Eastern Cooperative Oncology Group (ECOG) Performance Status of 0 or 1.
  • Age 18 years or older at the time of study entry.
  • +7 more criteria

You may not qualify if:

  • Patients who because of age, general medical or psychiatric condition, or physiologic status cannot give valid informed consent.
  • Any condition that, in the opinion of the Principal Investigator, would impair the patient's ability to comply with study procedures.
  • Is currently pregnant or breastfeeding.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

University of California, San Francisco

San Francisco, California, 94143, United States

RECRUITING

MeSH Terms

Conditions

Prostatic NeoplasmsKidney NeoplasmsUrethral NeoplasmsLipodystrophy, Congenital Generalized

Interventions

Magnetic Resonance Spectroscopy

Condition Hierarchy (Ancestors)

Genital Neoplasms, MaleUrogenital NeoplasmsNeoplasms by SiteNeoplasmsGenital Diseases, MaleGenital DiseasesUrogenital DiseasesProstatic DiseasesMale Urogenital DiseasesUrologic NeoplasmsFemale Urogenital DiseasesFemale Urogenital Diseases and Pregnancy ComplicationsKidney DiseasesUrologic DiseasesUrethral DiseasesLipid Metabolism, Inborn ErrorsMetabolism, Inborn ErrorsGenetic Diseases, InbornCongenital, Hereditary, and Neonatal Diseases and AbnormalitiesLipodystrophySkin Diseases, MetabolicSkin DiseasesSkin and Connective Tissue DiseasesLipid Metabolism DisordersMetabolic DiseasesNutritional and Metabolic Diseases

Intervention Hierarchy (Ancestors)

Spectrum AnalysisChemistry Techniques, AnalyticalInvestigative Techniques

Study Officials

  • Rahul Aggarwal, MD

    University of California, San Francisco

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
DIAGNOSTIC
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR INVESTIGATOR
PI Title
Principal Investigator

Study Record Dates

First Submitted

May 24, 2023

First Posted

June 5, 2023

Study Start

May 25, 2023

Primary Completion (Estimated)

December 31, 2026

Study Completion (Estimated)

January 31, 2027

Last Updated

March 17, 2026

Record last verified: 2026-03

Data Sharing

IPD Sharing
Will not share

Locations