Effects of Dietary Nitrate Intake on Cardiovascular Performance in Response to Hand Grip Test in Bodybuilders

NCT05835401 | Completed

• 1 other identifier: BodyBeet
• Study Type: interventional
• Target: 13
• Locations: 1 country
• Sites: 1

Brief Summary

Through a randomized, crossover, double-blind, placebo-controlled clinical trial, 20 bodybuilders will participate a acute-day trial with two intervention protocols: 1) placebo and 2) nitrate; in which will ingest beet juice with or without NO3 in its composition with a 7-day washout interval.

Conditions

Steroid Abuse; Cardiovascular Abnormalities

Outcome Measures

Primary Outcomes (1)

• 1. Values of systolic and diastolic blood pressure (mmHg)

  up to 2 years

Secondary Outcomes (5)

• Plasmatic concentrations of nitrate and nitrite (mmol/L)

  up to 2 years

• Values of heart rate variability (ms)

  up to 2 years

• Macrovascular function (Flow mediated dilatation%)

  up to 2 years

• Values of heart rate (beats per minute)

  up to 2 years

• Values of hand grip strength (kgF)

  up to 2 years

Study Arms (2)

Experimental (Beetroot juice NO3)

EXPERIMENTAL

Acute ingestion of beetroot juice rich in NO3 (800mg).

Dietary Supplement: Beetroot juice rich in nitrate

Placebo (Beetroot juice without NO3)

PLACEBO COMPARATOR

Acute ingestion of beetroot juice rich depleted in NO3.

Dietary Supplement: Beetroot juice depleted in nitrate

Interventions

Beetroot juice rich in nitrate DIETARY_SUPPLEMENT

The participant will intake beetroot juice rich in NO3 (800mg). Two hours after intake the last bottle of juice, the participants will go to the laboratory for the handgrip and cardiovascular tests evaluation. Before crossover protocol a 7-day washout interval will be given for the purification of the compounds provided by the protocols.

Experimental (Beetroot juice NO3)

Beetroot juice depleted in nitrate DIETARY_SUPPLEMENT

The participant will intake beetroot juice depleted in NO3. Two hours after intake the last bottle of juice, the participants will go to the laboratory for the handgrip and cardiovascular tests evaluation. Before crossover protocol a 7-day washout interval will be given for the purification of the compounds provided by the protocols.

Placebo (Beetroot juice without NO3)

Eligibility Criteria

• Age: 18 Years - 45 Years
• Sex: male
• Healthy Volunteers: No
• Age Groups: Adult (18-64)

You may qualify if:

• Anabolic steroid use in the past or actually;
• Participation at least one bodybuilding competition;

You may not qualify if:

• Previous history of acute myocardial infarction and/or stroke;
• Allergy or intolerance to nitrate, gluten, or milk;
• Medications (proton pump inhibitors, beta-blockers, calcium channels antagonists).

Sponsors & Collaborators

• University of Sao Paulo: lead

Study Sites (1)

EEFERP - USP / Laboratório de Fisiologia do Exercício e Metabolismo

Ribeirão Preto, São Paulo, Brazil

Location

MeSH Terms

Conditions

Cardiovascular Abnormalities

Interventions

Nitrates

Condition Hierarchy (Ancestors)

Cardiovascular Diseases; Congenital Abnormalities; Congenital, Hereditary, and Neonatal Diseases and Abnormalities

Intervention Hierarchy (Ancestors)

Anions; Ions; Electrolytes; Inorganic Chemicals; Nitric Acid; Nitrogen Compounds; Organic Chemicals

Study Officials

• Cicero Jonas Rodrigues Benjamim, PhD Student

  University of Sao Paulo

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Purpose: SUPPORTIVE CARE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal investigator

Study Record Dates

• First Submitted: April 18, 2023
• First Posted: April 28, 2023
• Study Start: April 17, 2023
• Primary Completion: December 31, 2023
• Study Completion: December 31, 2023
• Last Updated: July 28, 2025

Record last verified: 2025-07

Data Sharing

• IPD Sharing: Will not share

Locations

BR (1)