Selenoproteins as Prognostic Markers and Therapeutic Targets in Breast Cancer.

NCT05817227 | Recruiting

• 2 other identifiers: SELEBREC57/21oss
• Study Type: observational
• Target: 300
• Locations: 1 country
• Sites: 2

Brief Summary

This is multicentric, spontaneous, observational, retrospective and prospective study.

Conditions

Breast Cancer

Outcome Measures

Primary Outcomes (2)

• Use of immunohistochemistry investigation (IHC)

  In the retrospective part of the study, Tissue Microarrays (TMA) will be prepared from surgical samples of breast tissue from a series of patients with TNBC type breast cancer. The expression of some of the twenty-five selenoproteins will be evaluated by immunohistochemical investigation (IHC) on the TMA (Tumor Microarrays).

  3 years

• Prospective evaluation of expression of some selenoproteins by kit ELISA in plasma samples

  In the prospective part of the study will be evaluated the expression of some selenoproteins by ELISA in plasma samples obtained from up to 100 healthy subjects and about 300 (\~100 per year) patients with basally metastatic TNBC breast cancer (150) and metastatic (150) before any treatment.

  3 years

Eligibility Criteria

• Age: 18 Years+
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

The patients included in the study are all women and have an age below 40 in 26/235 (11.1%) cases, between 40 and 60 in 108/235 (46.0%) cases and over 60 for 101/235 (43.0%) cases. Tumours have the following clinical and pathological characteristics: 204/235 (86.8%) are carcinomiducts and 31/235 (13.2%) are non ductal carcinomas; 202/235 (86%) grade 3, 31/235 (13.1%) grade 2 and 2/235 (0.9%) grade 1; tumour dimensions are less than 2 cm (T1) in 103/225 cases (45.8%), between 2 and 5 cm in 103/225 (45.8%) and greater than 5 cm in 19/225 (8.4%). Expression of Ki67 diproliferation factor was high (\>20%) in 186/227 (81.9%) patients and low (\<20%) in 41/227 (18.1%). At the date of surgery 97/232 (41.8%) patients had lymphonode metastases, while 44/111 (39.6%) had distant metastases.

You may qualify if:

• TNBC breast cancer patients, before any drug treatment
• healthy women aged 25-60

You may not qualify if:

• donors suffering from diabetes, hypertension, active infectious states, HIV infection, Hepatitis B or C, chronic inflammatory diseases, current or previous neoplasms, heart disease or drug treatment.

Sponsors & Collaborators

• National Cancer Institute, Naples: lead

Study Sites (2)

Istituto Nazionale Tumori - Fondazione "G.Pascale", IRCCS

Naples, Napoli, 80131, Italy

RECRUITING

Istituto Nazionale Tumori Regina Elena

Roma, Roma, 00144, Italy

RECRUITING

Related Publications (22)

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• DeSantis CE, Ma J, Gaudet MM, Newman LA, Miller KD, Goding Sauer A, Jemal A, Siegel RL. Breast cancer statistics, 2019. CA Cancer J Clin. 2019 Nov;69(6):438-451. doi: 10.3322/caac.21583. Epub 2019 Oct 2.

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• Garrido-Castro AC, Lin NU, Polyak K. Insights into Molecular Classifications of Triple-Negative Breast Cancer: Improving Patient Selection for Treatment. Cancer Discov. 2019 Feb;9(2):176-198. doi: 10.1158/2159-8290.CD-18-1177. Epub 2019 Jan 24.

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• Varlamova EG. Participation of selenoproteins localized in the ER in the processes occurring in this organelle and in the regulation of carcinogenesis-associated processes. J Trace Elem Med Biol. 2018 Jul;48:172-180. doi: 10.1016/j.jtemb.2018.04.005. Epub 2018 Apr 3.

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• Guariniello S, Di Bernardo G, Colonna G, Cammarota M, Castello G, Costantini S. Evaluation of the selenotranscriptome expression in two hepatocellular carcinoma cell lines. Anal Cell Pathol (Amst). 2015;2015:419561. doi: 10.1155/2015/419561. Epub 2015 Jun 23.

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  PMID: 32933107 BACKGROUND

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  PMID: 12697962 BACKGROUND

• Debski MG, Pachucki J, Ambroziak M, Olszewski W, Bar-Andziak E. Human breast cancer tissue expresses high level of type 1 5'-deiodinase. Thyroid. 2007 Jan;17(1):3-10. doi: 10.1089/thy.2006.0012.

  PMID: 17274741 BACKGROUND

• Cha MK, Suh KH, Kim IH. Overexpression of peroxiredoxin I and thioredoxin1 in human breast carcinoma. J Exp Clin Cancer Res. 2009 Jun 30;28(1):93. doi: 10.1186/1756-9966-28-93.

  PMID: 19566940 BACKGROUND

• Pellatt AJ, Wolff RK, John EM, Torres-Mejia G, Hines LM, Baumgartner KB, Giuliano AR, Lundgreen A, Slattery ML. SEPP1 influences breast cancer risk among women with greater native american ancestry: the breast cancer health disparities study. PLoS One. 2013 Nov 20;8(11):e80554. doi: 10.1371/journal.pone.0080554. eCollection 2013.

  PMID: 24278290 BACKGROUND

• Ravn-Haren G, Olsen A, Tjonneland A, Dragsted LO, Nexo BA, Wallin H, Overvad K, Raaschou-Nielsen O, Vogel U. Associations between GPX1 Pro198Leu polymorphism, erythrocyte GPX activity, alcohol consumption and breast cancer risk in a prospective cohort study. Carcinogenesis. 2006 Apr;27(4):820-5. doi: 10.1093/carcin/bgi267. Epub 2005 Nov 14.

  PMID: 16287877 BACKGROUND

• Hu YJ, Diamond AM. Role of glutathione peroxidase 1 in breast cancer: loss of heterozygosity and allelic differences in the response to selenium. Cancer Res. 2003 Jun 15;63(12):3347-51.

  PMID: 12810669 BACKGROUND

• Knight JA, Onay UV, Wells S, Li H, Shi EJ, Andrulis IL, Ozcelik H. Genetic variants of GPX1 and SOD2 and breast cancer risk at the Ontario site of the Breast Cancer Family Registry. Cancer Epidemiol Biomarkers Prev. 2004 Jan;13(1):146-9. doi: 10.1158/1055-9965.epi-03-0164.

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• Rusolo F, Capone F, Pasquale R, Angiolillo A, Colonna G, Castello G, Costantini M, Costantini S. Comparison of the seleno-transcriptome expression between human non-cancerous mammary epithelial cells and two human breast cancer cell lines. Oncol Lett. 2017 Apr;13(4):2411-2417. doi: 10.3892/ol.2017.5715. Epub 2017 Feb 13.

  PMID: 28454412 BACKGROUND

• Nunziata C, Polo A, Sorice A, Capone F, Accardo M, Guerriero E, Marino FZ, Orditura M, Budillon A, Costantini S. Structural analysis of human SEPHS2 protein, a selenocysteine machinery component, over-expressed in triple negative breast cancer. Sci Rep. 2019 Nov 6;9(1):16131. doi: 10.1038/s41598-019-52718-0.

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• Costantini S, Capone F, Polo A, Bagnara P, Budillon A. Valosin-Containing Protein (VCP)/p97: A Prognostic Biomarker and Therapeutic Target in Cancer. Int J Mol Sci. 2021 Sep 21;22(18):10177. doi: 10.3390/ijms221810177.

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• Epplein M, Burk RF, Cai Q, Hargreaves MK, Blot WJ. A prospective study of plasma Selenoprotein P and lung cancer risk among low-income adults. Cancer Epidemiol Biomarkers Prev. 2014 Jul;23(7):1238-44. doi: 10.1158/1055-9965.EPI-13-1308. Epub 2014 Apr 24.

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• Yu SS, Du JL. Selenoprotein S: a therapeutic target for diabetes and macroangiopathy? Cardiovasc Diabetol. 2017 Aug 10;16(1):101. doi: 10.1186/s12933-017-0585-8.

  PMID: 28797256 BACKGROUND

MeSH Terms

Conditions

Breast Neoplasms

Condition Hierarchy (Ancestors)

Neoplasms by Site; Neoplasms; Breast Diseases; Skin Diseases; Skin and Connective Tissue Diseases

Study Officials

• Alfredo Budillon, M.D.

  IRCCS I.N.T. "G. Pascale"

  PRINCIPAL INVESTIGATOR

• Susan Costantini, PhD

  IRCCS I.N.T. "G. Pascale"

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Elena Di Gennaro, PhD

CONTACT

08117770584; e.digennaro@istitutotumori.na.it

Susan Costantini, PhD

CONTACT

08251911729; s.costantini@istitutotumori.na.it

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: OTHER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: March 22, 2023
• First Posted: April 18, 2023
• Study Start: May 19, 2022
• Primary Completion (Estimated): November 19, 2027
• Study Completion (Estimated): November 19, 2027
• Last Updated: December 22, 2025

Record last verified: 2025-07

Locations

IT (2)